Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 salmonella genomic sequences, and genotyping of salmonellae by using PCR.

abstract：:Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...

journal_title：Applied and environmental microbiology

authors： Purevdorj B,Costerton JW,Stoodley P

更新日期：2002-09-01 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the ...

journal_title：Applied and environmental microbiology

authors： Viña-Gonzalez J,Gonzalez-Perez D,Ferreira P,Martinez AT,Alcalde M

更新日期：2015-09-01 00:00:00

abstract：:Representatives of Actinobacteria were isolated from the marine sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic ...

journal_title：Applied and environmental microbiology

authors： Schneemann I,Nagel K,Kajahn I,Labes A,Wiese J,Imhoff JF

更新日期：2010-06-01 00:00:00

abstract：:Processes by which fecal bacteria enter overland flow and their transportation state to surface waters are poorly understood, making the effectiveness of measures designed to intercept this pathway, such as vegetated buffer strips, difficult to predict. Freshly made and aged (up to 30 days) cowpats were exposed to sim...

journal_title：Applied and environmental microbiology

authors： Muirhead RW,Collins RP,Bremer PJ

更新日期：2005-06-01 00:00:00

abstract：:Inhibition of enzyme activity by high concentrations of substrate and/or cofactor is a general phenomenon demonstrated in many enzymes, including aldehyde dehydrogenases. Here we show that the uncharacterized protein BetB (SA2613) from Staphylococcus aureus is a highly specific betaine aldehyde dehydrogenase, which ex...

journal_title：Applied and environmental microbiology

authors： Chen C,Joo JC,Brown G,Stolnikova E,Halavaty AS,Savchenko A,Anderson WF,Yakunin AF

更新日期：2014-07-01 00:00:00

abstract：:The anoxygenic phototrophic bacterium Rhodopseudomonas palustris produces methane (CH4) from carbon dioxide (CO2) and hydrogen (H2) from protons (H+) when it expresses a variant form of molybdenum (Mo) nitrogenase that has two amino acid substitutions near its active site. We examined the influence of light energy and...

journal_title：Applied and environmental microbiology

authors： Zheng Y,Harwood CS

更新日期：2019-04-18 00:00:00

abstract：:Silver toxicity is a problem that microorganisms face in medical and environmental settings. Through exposure to silver compounds, some bacteria have adapted to growth in high concentrations of silver ions. Such adapted microbes may be dangerous as pathogens but, alternatively, could be potentially useful in nanomater...

journal_title：Applied and environmental microbiology

authors： Sedlak RH,Hnilova M,Grosh C,Fong H,Baneyx F,Schwartz D,Sarikaya M,Tamerler C,Traxler B

更新日期：2012-04-01 00:00:00

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:The potential of bacteriophage as an alternative biocontrol agent has recently been revisited due to the widespread occurrence of antibiotic-resistant bacteria. We isolated a virulent bacteriophage, SPC35, that can infect both Salmonella enterica serovar Typhimurium and Escherichia coli. Morphological analysis by tran...

journal_title：Applied and environmental microbiology

authors： Kim M,Ryu S

更新日期：2011-03-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural a...

journal_title：Applied and environmental microbiology

authors： Joris MA,Vanrompay D,Verstraete K,De Reu K,De Zutter L,Cox E

更新日期：2013-06-01 00:00:00

abstract：:Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a st...

journal_title：Applied and environmental microbiology

authors： Caruso C,Rizzo C,Mangano S,Poli A,Di Donato P,Finore I,Nicolaus B,Di Marco G,Michaud L,Lo Giudice A

更新日期：2018-01-31 00:00:00

abstract：:Hepatitis B virus (HBV) is a deadly pathogen that has killed countless people worldwide. Saccharomyces cerevisiae-derived HBV vaccines based upon hepatitis B surface antigen (HBsAg) is highly effective. However, the emergence of vaccine escape mutants due to mutations on the HBsAg and polymerase genes has produced a c...

journal_title：Applied and environmental microbiology

authors： Yong CY,Yeap SK,Goh ZH,Ho KL,Omar AR,Tan WS

更新日期：2015-02-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we dis...

journal_title：Applied and environmental microbiology

authors： Rapp C,van Overtveldt S,Beerens K,Weber H,Desmet T,Nidetzky B

更新日期：2020-12-04 00:00:00

abstract：:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition as...

journal_title：Applied and environmental microbiology

authors： Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré J

更新日期：2012-06-01 00:00:00

abstract：:Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-alpha2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-density cultivat...

journal_title：Applied and environmental microbiology

authors： Sletta H,Tøndervik A,Hakvåg S,Aune TE,Nedal A,Aune R,Evensen G,Valla S,Ellingsen TE,Brautaset T

更新日期：2007-02-01 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

journal_title：Applied and environmental microbiology

authors： Picardeau M

更新日期：2008-01-01 00:00:00

abstract：:Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5 Tnp transposon library. ...

journal_title：Applied and environmental microbiology

authors： Guo Y,Sagaram US,Kim JS,Wang N

更新日期：2010-04-01 00:00:00

abstract：:o-Methoxycinnamaldehyde has been isolated and purified from powdered cinnamon. The compound inhibits the growth and toxin production of mycotoxin-producing fungi. The substance completely inhibited the growth of Aspergillus parasiticus and A. flavus at 100 microgram/ml and A. ochraceus and A. versicolor at 200 microgr...

journal_title：Applied and environmental microbiology

authors： Morozumi S

更新日期：1978-10-01 00:00:00

abstract：:In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, wh...

journal_title：Applied and environmental microbiology

authors： Cocolin L,Urso R,Rantsiou K,Cantoni C,Comi G

更新日期：2006-01-01 00:00:00

abstract：:Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...

journal_title：Applied and environmental microbiology

authors： Amner W,McCarthy AJ,Edwards C

更新日期：1988-12-01 00:00:00

abstract：:1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli express...

journal_title：Applied and environmental microbiology

authors： Altaras NE,Cameron DC

更新日期：1999-03-01 00:00:00

abstract：:Pseudomonas aeruginosa JB2 was isolated from a polychlorinated biphenyl-contaminated soil by enrichment culture containing 2-chlorobenzoate as the sole carbon source. Strain JB2 was subsequently found also to grow on 3-chlorobenzoate, 2,3- and 2,5-dichlorobenzoates, 2,3,5-trichlorobenzoate, and a wide range of other m...

journal_title：Applied and environmental microbiology

authors： Hickey WJ,Focht DD

更新日期：1990-12-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. ...

journal_title：Applied and environmental microbiology

authors： Regalado V,Rodriguez A,Perestelo F,Carnicero A,De La Fuente G,Falcon MA

更新日期：1997-09-01 00:00:00

abstract：:Two alpha-amylase genes from the thermophilic alkaliphile Anaerobranca gottschalkii were cloned, and the corresponding enzymes, AmyA and AmyB, were investigated after purification of the recombinant proteins. Based on their amino acid sequences, AmyA is proposed to be a lipoprotein with extracellular localization and ...

journal_title：Applied and environmental microbiology

authors： Ballschmiter M,Armbrecht M,Ivanova K,Antranikian G,Liebl W

更新日期：2005-07-01 00:00:00

abstract：:An efficient method for the transformation of the methylotrophic bacterium Methylobacterium extorquens NR-2 with a broad-host-range plasmid, pLA2917, by electroporation was examined. Transformants of M. extorquens NR-2 expressing resistance to kanamycin were obtained after electric pulse. These transformants were foun...

journal_title：Applied and environmental microbiology

authors： Ueda S,Matsumoto S,Shimizu S,Yamane T

更新日期：1991-04-01 00:00:00