Comprehensive investigation of marine Actinobacteria associated with the sponge Halichondria panicea.

Abstract:

:Representatives of Actinobacteria were isolated from the marine sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic exploration of biosynthetic genes, especially concerning the relationships of the abundance of biosynthesis gene fragments to the number and diversity of produced secondary metabolites. All strains were phylogenetically identified by 16S rRNA gene sequence analyses and were found to belong to the genera Actinoalloteichus, Micrococcus, Micromonospora, Nocardiopsis, and Streptomyces. Secondary metabolite profiles of 46 actinobacterial strains were evaluated, 122 different substances were identified, and 88 so far unidentified compounds were detected. The extracts from most of the cultures showed biological activities. In addition, the presence of biosynthesis genes encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) in 30 strains was established. It was shown that strains in which either PKS or NRPS genes were identified produced a significantly higher number of metabolites and exhibited a larger number of unidentified, possibly new metabolites than other strains. Therefore, the presence of PKS and NRPS genes is a good indicator for the selection of strains to isolate new natural products.

journal_name

Appl Environ Microbiol

authors

Schneemann I,Nagel K,Kajahn I,Labes A,Wiese J,Imhoff JF

doi

10.1128/AEM.00780-10

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

3702-14

issue

11

eissn

0099-2240

issn

1098-5336

pii

AEM.00780-10

journal_volume

76

pub_type

杂志文章
  • Growth of geobacter sulfurreducens with acetate in syntrophic cooperation with hydrogen-oxidizing anaerobic partners

    abstract::Pure cultures of Geobacter sulfurreducens and other Fe(III)-reducing bacteria accumulated hydrogen to partial pressures of 5 to 70 Pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electron donor if electron release to an acceptor was limiting. G. sulfurreducens coupled acetate oxidation with el...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.6.2232-2236.1998

    authors: Cord-Ruwisch R,Lovley DR,Schink B

    更新日期:1998-06-01 00:00:00

  • Inexpensive laboratory-constructed nephelometer.

    abstract::An inexpensive, simple-to-construct nephelometer which was used to monitor the lysis of spheroplasts is described. The nephelometer is a flow-through device with a linear response to cell concentration from the lower detection limit to 8 x 10(8) cells per ml. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.6.1476-1478.1982

    authors: Smith TL,Witter LD

    更新日期:1982-12-01 00:00:00

  • Phytic Acid and Sodium Chloride Show Marked Synergistic Bactericidal Effects against Nonadapted and Acid-Adapted Escherichia coli O157:H7 Strains.

    abstract::The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much gre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03307-15

    authors: Kim NH,Rhee MS

    更新日期:2015-12-04 00:00:00

  • Expression of a tetraheme protein, Desulfovibrio vulgaris Miyazaki F cytochrome c(3), in Shewanella oneidensis MR-1.

    abstract::Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.4168-4171.2000

    authors: Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

    更新日期:2000-09-01 00:00:00

  • Presence of enterohemorrhagic Escherichia coli ST678/O104:H4 in France prior to 2011.

    abstract::Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06524-11

    authors: Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

    更新日期:2011-12-01 00:00:00

  • Dual substrate specificity of an N-acetylglucosamine phosphotransferase system in Clostridium beijerinckii.

    abstract::The solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. In common with other anaerobes, the clostridia show a marked dependence on the phosphoeno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01866-13

    authors: Al Makishah NH,Mitchell WJ

    更新日期:2013-11-01 00:00:00

  • Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans.

    abstract::Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03191-13

    authors: Cohen-Rosenzweig C,Guan Z,Shaanan B,Eichler J

    更新日期:2014-01-01 00:00:00

  • Characterization of novel polycyclic aromatic hydrocarbon dioxygenases from the bacterial metagenomic DNA of a contaminated soil.

    abstract::Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01883-14

    authors: Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

    更新日期:2014-11-01 00:00:00

  • Acetate Degradation at 70 degrees C in Upflow Anaerobic Sludge Blanket Reactors and Temperature Response of Granules Grown at 70 degrees C.

    abstract::Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, correspondi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.6.1742-1746.1993

    authors: Rintala J,Lepistö S,Ahring B

    更新日期:1993-06-01 00:00:00

  • Production and Purification of Thermostable Amylase and Protease of Thermomonospora viridis.

    abstract::Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.1.59-64.1977

    authors: Upton ME,Fogarty WM

    更新日期:1977-01-01 00:00:00

  • Respiration and growth of Shewanella decolorationis S12 with an Azo compound as the sole electron acceptor.

    abstract::The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01415-06

    authors: Hong Y,Xu M,Guo J,Xu Z,Chen X,Sun G

    更新日期:2007-01-01 00:00:00

  • Feral Swine in the United States Have Been Exposed to both Avian and Swine Influenza A Viruses.

    abstract::Influenza A viruses (IAVs) in swine can cause sporadic infections and pandemic outbreaks among humans, but how avian IAV emerges in swine is still unclear. Unlike domestic swine, feral swine are free ranging and have many opportunities for IAV exposure through contacts with various habitats and animals, including migr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01346-17

    authors: Martin BE,Sun H,Carrel M,Cunningham FL,Baroch JA,Hanson-Dorr KC,Young SG,Schmit B,Nolting JM,Yoon KJ,Lutman MW,Pedersen K,Lager K,Bowman AS,Slemons RD,Smith DR,DeLiberto T,Wan XF

    更新日期:2017-09-15 00:00:00

  • Resuscitation by ferrioxamine E of stressed Salmonella enterica serovar typhimurium from soil and water microcosms.

    abstract::Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.4128-4130.2000

    authors: Reissbrodt R,Heier H,Tschäpe H,Kingsley RA,Williams PH

    更新日期:2000-09-01 00:00:00

  • Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants.

    abstract::Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.2.553-556.1991

    authors: Schesser K,Luder A,Henson JM

    更新日期:1991-02-01 00:00:00

  • Biomass and Biological Activity during the Production of Compost Used as a Substrate in Mushroom Cultivation.

    abstract::The production of a suitable substrate for the cultivation of the common white button mushroom, Agaricus bisporus, is referred to as composting. High microbiological activity causes temperatures of the composting material to rise as high as 80 degrees C. At stacking, an optimal oxygen consumption rate of 140 mumol of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.10.3029-3034.1990

    authors: Derikx PJ,Op Den Camp HJ,van der Drift C,Van Griensven LJ,Vogels GD

    更新日期:1990-10-01 00:00:00

  • Comparison of effects of compost amendment and of single-strain inoculation on root bacterial communities of young cucumber seedlings.

    abstract::Compost amendment and inoculations with specific microorganisms are fundamentally different soil treatment methods, commonly used in agriculture for the improvement of plant growth and health. Although distinct, both methods affect the rhizosphere and the plant roots. In the present study we used a 16S rRNA gene appro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00736-09

    authors: Ofek M,Hadar Y,Minz D

    更新日期:2009-10-01 00:00:00

  • Effects of pulsed electric fields on inactivation kinetics of Listeria innocua.

    abstract::The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5364-5371.1999

    authors: Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

    更新日期:1999-12-01 00:00:00

  • Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis.

    abstract::A soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. By standard bacteriological methods, this bacterium was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.1079-1086.1990

    authors: Mueller JG,Chapman PJ,Blattmann BO,Pritchard PH

    更新日期:1990-04-01 00:00:00

  • Bacterial activity associated with the decomposition of woody substrates in a stream sediment.

    abstract::Ground bark and heartwood from Alnus rubra and Pseudotsuga menziesii were added to a muddy sediment from a small Oregon stream and incubated in situ. Carbon dioxide and methane production rates were increased by all amendments, the biggest increase being shown with A. rubra wood. Except for sediment amended with A. ru...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.2.516-521.1983

    authors: Baker JH,Morita RY,Anderson NH

    更新日期:1983-02-01 00:00:00

  • Citrate, a specific substrate for the isolation of Clostridium sphenoides.

    abstract::With a medium containing citrate as the carbon and energy source, 10 clostridial strains were isolated from various mud samples. Characterization of these strains revealed that they all belonged to the same species, Clostridium sphenoides. Strains of this organism obtained from culture collections were also able to gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.4.955-962.1977

    authors: Walther R,Hippe H,Gottschalk G

    更新日期:1977-04-01 00:00:00

  • Long-term population dynamics of phototrophic sulfur bacteria in the chemocline of Lake Cadagno, Switzerland.

    abstract::Population analyses in water samples obtained from the chemocline of crenogenic, meromictic Lake Cadagno, Switzerland, in October for the years 1994 to 2003 were studied using in situ hybridization with specific probes. During this 10-year period, large shifts in abundance between purple and green sulfur bacteria and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.3544-3550.2005

    authors: Tonolla M,Peduzzi R,Hahn D

    更新日期:2005-07-01 00:00:00

  • Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses.

    abstract::Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4200-4204.2003

    authors: Halpern M,Gancz H,Broza M,Kashi Y

    更新日期:2003-07-01 00:00:00

  • A Novel Glaesserella sp. Isolated from Pigs with Severe Respiratory Infections Has a Mosaic Genome with Virulence Factors Putatively Acquired by Horizontal Transfer.

    abstract::An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00092-18

    authors: Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

    更新日期:2018-05-17 00:00:00

  • Microbial reduction of Fe(III) in acidic sediments: isolation of Acidiphilium cryptum JF-5 capable of coupling the reduction of Fe(III) to the oxidation of glucose.

    abstract::To evaluate the microbial populations involved in the reduction of Fe(III) in an acidic, iron-rich sediment, the anaerobic flow of supplemental carbon and reductant was evaluated in sediment microcosms at the in situ temperature of 12 degrees C. Supplemental glucose and cellobiose stimulated the formation of Fe(II); 4...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3633-3640.1999

    authors: Küsel K,Dorsch T,Acker G,Stackebrandt E

    更新日期:1999-08-01 00:00:00

  • Lyophilization of rumen fluid for use in culture media.

    abstract::The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.12.3237-3239.1989

    authors: Dehority BA,Tirabasso PA

    更新日期:1989-12-01 00:00:00

  • Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei.

    abstract::Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01742-17

    authors: Kiesenhofer DP,Mach RL,Mach-Aigner AR

    更新日期:2017-12-15 00:00:00

  • Production and secretion in Escherichia coli of hepatitis B virus pre-S2 antigen as fusion proteins with beta-lactamase.

    abstract::The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2742-2747.1990

    authors: Kadokura H,Yoda K,Imai M,Yamasaki M

    更新日期:1990-09-01 00:00:00

  • BopA does not have a major role in the adhesion of Bifidobacterium bifidum to intestinal epithelial cells, extracellular matrix proteins, and mucus.

    abstract::The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was pre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01993-13

    authors: Kainulainen V,Reunanen J,Hiippala K,Guglielmetti S,Vesterlund S,Palva A,Satokari R

    更新日期:2013-11-01 00:00:00

  • Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment.

    abstract::We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.1.233-240.2003

    authors: Sampaio MM,Santos H,Boos W

    更新日期:2003-01-01 00:00:00

  • Microfluidic quantitative PCR for simultaneous quantification of multiple viruses in environmental water samples.

    abstract::To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02578-14

    authors: Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

    更新日期:2014-12-01 00:00:00