Effects of pulsed electric fields on inactivation kinetics of Listeria innocua.

Abstract:

:The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most significant process factors influencing the inactivation kinetics. Product factors (pH and conductivity) also influenced the inactivation kinetics. In phosphate buffer at pH 4.0 and 0.5 S/m at 40 degrees C, a 3. 0-V/microm PEF treatment at an inlet temperature of 40 degrees C resulted in > or = 6.3 log inactivation of strain NCTC 11289 at 49.5 degrees C. A synergistic effect between temperature and PEF inactivation was also observed. The inactivation obtained with PEF was compared to the inactivation obtained with heat. We found that heat inactivation was less effective than PEF inactivation under similar time and temperature conditions. L. innocua cells which were incubated for a prolonged time in the stationary phase were more resistant to the PEF treatment, indicating that the physiological state of the microorganism plays a role in inactivation by PEF. Sublethal injury of cells was observed after PEF treatment, and the injury was more severe when the level of treatment was increased. Overall, our results indicate that it may be possible to use PEF in future applications in order to produce safe products.

journal_name

Appl Environ Microbiol

authors

Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

doi

10.1128/AEM.65.12.5364-5371.1999

keywords:

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

5364-71

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

杂志文章
  • Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii.

    abstract::The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled wi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01726-07

    authors: Mullane NR,Ryan M,Iversen C,Murphy M,O'Gaora P,Quinn T,Whyte P,Wall PG,Fanning S

    更新日期:2008-02-01 00:00:00

  • Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay.

    abstract::A new detection system, the magnetic immuno-polymerase chain reaction (PCR) assay (MIPA) has been developed to detect Listeria monocytogenes in food. This method separates Listeria cells from PCR-inhibitory factors present in enrichment broths containing food samples by using magnetic beads coated with specific monocl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.5.1289-1293.1993

    authors: Fluit AC,Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,Keller BH,Klapwijk P,Verhoef J

    更新日期:1993-05-01 00:00:00

  • Effects of nutrients on specific growth rate of bacterioplankton in oligotrophic lake water cultures.

    abstract::The effects of organic and inorganic nutrient additions on the specific growth rates of bacterioplankton in oligotrophic lake water cultures were investigated. Lake water was first passed through 0.8-mum-pore-size filters (prescreening) to remove bacterivores and to minimize confounding effects of algae. Specific grow...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.1.150-156.1992

    authors: Coveney MF,Wetzel RG

    更新日期:1992-01-01 00:00:00

  • Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives.

    abstract::A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02076-14

    authors: Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

    更新日期:2014-12-01 00:00:00

  • Mass and density measurements of live and dead Gram-negative and Gram-positive bacterial populations.

    abstract::Monitoring cell growth and measuring physical features of food-borne pathogenic bacteria are important for better understanding the conditions under which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead Escherichia coli O157:H7 and Listeria innocua were measured usin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00117-14

    authors: Lewis CL,Craig CC,Senecal AG

    更新日期:2014-06-01 00:00:00

  • Biosurfactant production by a soil pseudomonas strain growing on polycyclic aromatic hydrocarbons.

    abstract::The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.1908-1912.1996

    authors: Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

    更新日期:1996-06-01 00:00:00

  • Recovery of spores of Clostridium botulinum in yeast extract agar and pork infusion agar after heat treatment.

    abstract::Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.4.377-381.1977

    authors: Odlaug TE,Pflug IJ

    更新日期:1977-10-01 00:00:00

  • Effects of pH of the medium on flagellation of Vibrio parahaemolyticus.

    abstract::Formation of the lateral flagella by Vibrio parahaemolyticus was inhibited under an alkaline condition of the medium. However, flagellation of the polar monotrichous flagellum was not affected in the same condition. Flagellation of the lateral flagella depended on the pH of the medium. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.6.1248-1249.1979

    authors: Kimura K,Tateiri S,Iida H

    更新日期:1979-06-01 00:00:00

  • Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility.

    abstract::We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01027-13

    authors: Qian C,Wong CC,Swarup S,Chiam KH

    更新日期:2013-08-01 00:00:00

  • Characterization of geographically distinct bacterial communities associated with coral mucus produced by Acropora spp. and Porites spp.

    abstract::Acropora and Porites corals are important reef builders in the Indo-Pacific and Caribbean. Bacteria associated with mucus produced by Porites spp. and Acropora spp. from Caribbean (Punta Maroma, Mexico) and Indo-Pacific (Hoga and Sampela, Indonesia) reefs were determined. Analysis of pyrosequencing libraries showed th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07764-11

    authors: McKew BA,Dumbrell AJ,Daud SD,Hepburn L,Thorpe E,Mogensen L,Whitby C

    更新日期:2012-08-01 00:00:00

  • Divergent Co-occurrence Patterns and Assembly Processes Structure the Abundant and Rare Bacterial Communities in a Salt Marsh Ecosystem.

    abstract::Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00322-20

    authors: Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

    更新日期:2020-06-17 00:00:00

  • Documenting the epidemiologic patterns of polyomaviruses in human populations by studying their presence in urban sewage.

    abstract::This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage. Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BK virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.238-245.2000

    authors: Bofill-Mas S,Pina S,Girones R

    更新日期:2000-01-01 00:00:00

  • Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.

    abstract::Six strains of a new species, Legionella sainthelensi, were isolated from freshwater in areas affected by the volcanic eruptions of Mt. St. Helens in the state of Washington. Strains of L. sainthelensi are culturally and biochemically similar to other legionellae. They grow on buffered charcoal yeast agar but not on m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.2.369-373.1984

    authors: Campbell J,Bibb WF,Lambert MA,Eng S,Steigerwalt AG,Allard J,Moss CW,Brenner DJ

    更新日期:1984-02-01 00:00:00

  • Enhanced Growth of Wheat and Soybean Plants Inoculated with Azospirillum brasilense Is Not Necessarily Due to General Enhancement of Mineral Uptake.

    abstract::The capacity of Azospirillum brasilense to enhance the accumulation of K, P, Ca, Mg, S, Na, Mn, Fe, B, Cu, and Zn in inoculated wheat and soybean plants was evaluated by using two different analytical methods with five A. brasilense strains originating from four distinct geographical regions. A Pseudomonas isolate fro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.3.769-775.1990

    authors: Bashan Y,Harrison SK,Whitmoyer RE

    更新日期:1990-03-01 00:00:00

  • Rapid evaluation of the mycobactericidal efficacy of disinfectants in the quantitative carrier test EN 14563 by using fluorescent Mycobacterium terrae.

    abstract::To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01660-09

    authors: Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

    更新日期:2010-01-01 00:00:00

  • Effect of spatial differences in microbial activity, pH, and substrate levels on methanogenesis initiation in refuse.

    abstract::The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02349-10

    authors: Staley BF,de Los Reyes FL 3rd,Barlaz MA

    更新日期:2011-04-01 00:00:00

  • Influence of Ammonium on Formation of Mineral-Associated Organic Carbon by an Ectomycorrhizal Fungus.

    abstract::The interactions between dissolved organic matter (DOM) and mineral particles are critical for the stabilization of soil organic matter (SOM) in terrestrial ecosystems. The processing of DOM by ectomycorrhizal fungi contributes to the formation of mineral-stabilized SOM by two contrasting pathways: the extracellular t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03007-18

    authors: Wang T,Tian Z,Tunlid A,Persson P

    更新日期:2019-05-02 00:00:00

  • Inactivation of murine norovirus 1, coliphage phiX174, and Bacteroides [corrected] fragilis phage B40-8 on surfaces and fresh-cut iceberg lettuce by hydrogen peroxide and UV light.

    abstract::In this study, the inactivating properties of liquid hydrogen peroxide (L-H(2)O(2)), vaporized hydrogen peroxide (V-H(2)O(2)), UV light, and a combination of V-H(2)O(2) and UV light were tested on murine norovirus 1 (MNV-1) and bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02131-10

    authors: Li D,Baert L,De Jonghe M,Van Coillie E,Ryckeboer J,Devlieghere F,Uyttendaele M

    更新日期:2011-02-01 00:00:00

  • Evidence for iron-dependent nitrate respiration in the dissimilatory iron-reducing bacterium Geobacter metallireducens.

    abstract::The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximatel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3750-3752.2001

    authors: Senko JM,Stolz JF

    更新日期:2001-08-01 00:00:00

  • Cadmium Resistance Screening in Nitrilotriacetate-Buffered Minimal Media.

    abstract::Media used to determine the MICs of heavy metals for bacteria are unreliable because organic components in the media bind or chelate most of the metal being studied. To define specific metal activity in media and to maintain metal activity at a constant level, HEPES-MES [N-2-hydroxyethylpiperazine-N' -2-ethanesulfonic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.2101-2104.1989

    authors: Angle JS,Chaney RL

    更新日期:1989-08-01 00:00:00

  • Production and properties of cyanobacterial endotoxins.

    abstract::Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.104-109.1982

    authors: Keleti G,Sykora JL

    更新日期:1982-01-01 00:00:00

  • Bactericidal actions of a silver ion solution on Escherichia coli, studied by energy-filtering transmission electron microscopy and proteomic analysis.

    abstract::Bactericidal actions of the silver ion on Escherichia coli as a model microorganism were studied using energy-filtering transmission electron microscopy (EFTEM), two-dimensional electrophoresis (2-DE), and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). EFTEM observations d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.7589-7593.2005

    authors: Yamanaka M,Hara K,Kudo J

    更新日期:2005-11-01 00:00:00

  • Extensive reduction of cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with a D-amino acid mixture.

    abstract::Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02911-12

    authors: Sanchez Z,Tani A,Kimbara K

    更新日期:2013-02-01 00:00:00

  • Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for In vivo application of cytochrome P-450BM-3 monooxygenase.

    abstract::The application of whole cells containing cytochrome P-450BM-3 monooxygenase [EC 1.14.14.1] for the bioconversion of long-chain saturated fatty acids to omega-1, omega-2, and omega-3 hydroxy fatty acids was investigated. We utilized pentadecanoic acid and studied its conversion to a mixture of 12-, 13-, and 14-hydroxy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3784-3790.1998

    authors: Schneider S,Wubbolts MG,Sanglard D,Witholt B

    更新日期:1998-10-01 00:00:00

  • Effect of dietary carbohydrates on bacterial cholyltaurine hydrolase in poultry intestinal homogenates.

    abstract::The bile salt hydrolase activity in intestinal homogenates reflects composite activities of the gastrointestinal microbial consortia. We have proposed that specific transformations of conjugated bile acids by the intestinal microflora result in the production of metabolites which depress the growth of poultry. The inf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.337-342.1988

    authors: Feighner SD,Dashkevicz MP

    更新日期:1988-02-01 00:00:00

  • Development of a PCR-enzyme immunoassay oligoprobe detection method for Toxoplasma gondii oocysts, incorporating PCR controls.

    abstract::Infections caused by Toxoplasma gondii are widely prevalent in animals and humans throughout the world. In the United States, an estimated 23% of adolescents and adults have laboratory evidence of T. gondii infection. T. gondii has been identified as a major opportunistic pathogen in immunocompromised individuals, in ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.5819-5825.2003

    authors: Schwab KJ,McDevitt JJ

    更新日期:2003-10-01 00:00:00

  • Distribution of methanogenic and sulfate-reducing bacteria in near-shore marine sediments.

    abstract::The distribution of methanogenic and sulfate-reducing bacteria was examined in sediments from three sites off the coast of eastern Connecticut and five sites in Long Island Sound. Both bacterial groups were detected at all sites. Three distributional patterns were observed: (i) four sites exhibited methanogenic and su...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.447-453.1982

    authors: Hines ME,Buck JD

    更新日期:1982-02-01 00:00:00

  • Utilization of ganglioside-degrading Paenibacillus sp. strain TS12 for production of glucosylceramide.

    abstract::Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.11.5241-5248.2002

    authors: Sumida T,Sueyoshi N,Ito M

    更新日期:2002-11-01 00:00:00

  • C-ring cleavage of flavonoids by human intestinal bacteria.

    abstract::Four hitherto undescribed Clostridium strains capable of cleaving the C ring of quercetin, kaempferol, and naringenin at C-3-C-4 were isolated from the fecal flora of humans. None of the strains cleaved catechin. C-ring fission occurred when the substrate was either in solution or in suspension. Mixed cultures of flav...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.5.1203-1208.1989

    authors: Winter J,Moore LH,Dowell VR Jr,Bokkenheuser VD

    更新日期:1989-05-01 00:00:00

  • Inactivation Kinetics and Mechanism of a Human Norovirus Surrogate on Stainless Steel Coupons via Chlorine Dioxide Gas.

    abstract::Acute gastroenteritis caused by human norovirus is a significant public health issue. Fresh produce and seafood are examples of high-risk foods associated with norovirus outbreaks. Food contact surfaces also have the potential to harbor noroviruses if exposed to fecal contamination, aerosolized vomitus, or infected fo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02489-15

    authors: Yeap JW,Kaur S,Lou F,DiCaprio E,Morgan M,Linton R,Li J

    更新日期:2015-10-16 00:00:00