Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii.

abstract：:Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C...

journal_title：Applied and environmental microbiology

authors： Stinear T,Matusan A,Hines K,Sandery M

更新日期：1996-09-01 00:00:00

abstract：:To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

journal_title：Applied and environmental microbiology

authors： Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

更新日期：1990-05-01 00:00:00

abstract：:Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

journal_title：Applied and environmental microbiology

authors： LeCleir GR,Buchan A,Hollibaugh JT

更新日期：2004-12-01 00:00:00

abstract：:Recently, a new metabolic link between fatty acid de novo biosynthesis and biosynthesis of poly(3-hydroxy-alkanoate) consisting of medium-chain-length constituents (C(6) to C(14)) (PHA(MCL)), catalyzed by the 3-hydroxydecanoyl-[acyl-carrier-protein]:CoA transacylase (PhaG), has been identified in Pseudomonas putida (B...

journal_title：Applied and environmental microbiology

authors： Fiedler S,Steinbüchel A,Rehm BH

更新日期：2000-05-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-contai...

journal_title：Applied and environmental microbiology

authors： Berry D,Takach JE,Schardl CL,Charlton ND,Scott B,Young CA

更新日期：2015-04-01 00:00:00

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:The effects of preexposure of soybean (Glycine max L. Merrill) roots to Rhizobium japonicum strains and subsequent establishment of other strains in the nodules were investigated by using combinations of effective strains (USDA 110 and USDA 138) and effective-ineffective strains (USDA 110 and SM-5). Strain USDA 110 wa...

journal_title：Applied and environmental microbiology

authors： Kosslak RM,Bohlool BB,Dowdle S,Sadowsky MJ

更新日期：1983-10-01 00:00:00

abstract：:The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

journal_title：Applied and environmental microbiology

authors： Ben-Porath J,Zehr JP

更新日期：1994-03-01 00:00:00

abstract：:Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...

journal_title：Applied and environmental microbiology

authors： Rhodes LD,Coady AM,Deinhard RK

更新日期：2004-11-01 00:00:00

abstract：:Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detectable amounts of CH...

journal_title：Applied and environmental microbiology

authors： Harper DB,Buswell JA,Kennedy JT,Hamilton JT

更新日期：1990-11-01 00:00:00

abstract：:Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodu...

journal_title：Applied and environmental microbiology

authors： Laguerre G,Louvrier P,Allard MR,Amarger N

更新日期：2003-04-01 00:00:00

abstract：:Plasmodium falciparum, in addition to scavenging essential fatty acids from its intra- and intercellular environments, possesses a functional complement of type II fatty acid synthase (FAS) enzymes targeted to the apicoplast organelle. Recent evidence suggests that products of the plasmodial FAS II system may be criti...

journal_title：Applied and environmental microbiology

authors： Du Y,Gisselberg JE,Johnson JD,Lee PJ,Prigge ST,Bachmann BO

更新日期：2010-06-01 00:00:00

abstract：:A nonsporulating strain of Streptomyces diastaticus producing alpha-L-arabinofuranosidase activity (EC 3.2-1.55) was isolated from soil. Two alpha-L-arabinosidases were purified by ion-exchange chromatography and chromatofocusing. The enzymes had molecular weights of 38,000 (C1) and 60,000 (C2) and pIs of 8.8 and 8.3,...

journal_title：Applied and environmental microbiology

authors： Tajana E,Fiechter A,Zimmermann W

更新日期：1992-05-01 00:00:00

abstract：:Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and n...

journal_title：Applied and environmental microbiology

authors： Nassar A,Darrasse A,Lemattre M,Kotoujansky A,Dervin C,Vedel R,Bertheau Y

更新日期：1996-07-01 00:00:00

abstract：:Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial acti...

journal_title：Applied and environmental microbiology

authors： Norberg S,O'Connor PM,Stanton C,Ross RP,Hill C,Fitzgerald GF,Cotter PD

更新日期：2012-04-01 00:00:00

abstract：:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

journal_title：Applied and environmental microbiology

authors： Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

更新日期：2003-03-01 00:00:00

abstract：:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can ...

journal_title：Applied and environmental microbiology

authors： Haarman M,Knol J

更新日期：2006-04-01 00:00:00

abstract：:GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...

journal_title：Applied and environmental microbiology

authors： Lin CC,Casida LE

更新日期：1984-02-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:In this study, the changes in the global proteome of Salmonella in response to desiccation and thermal treatment were investigated by using an iTRAQ multiplex technique. A Salmonella enterica serovar Typhimurium strain was dried, equilibrated at high (1.0) and low (0.11) water activity (aw), and thermally treated at 7...

journal_title：Applied and environmental microbiology

authors： Maserati A,Lourenco A,Diez-Gonzalez F,Fink RC

更新日期：2018-08-31 00:00:00

abstract：:For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directl...

journal_title：Applied and environmental microbiology

authors： Löfblom J,Sandberg J,Wernérus H,Ståhl S

更新日期：2007-11-01 00:00:00

abstract：:There is a growing interest in the use of microbial fermentation for the generation of high-demand, high-purity chemicals using cheap feedstocks in an environmentally friendly manner. One example explored here is the production of isoprene (C5H8), a hemiterpene, which is primarily polymerized to polyisoprene in synthe...

journal_title：Applied and environmental microbiology

authors： Diner BA,Fan J,Scotcher MC,Wells DH,Whited GM

更新日期：2017-12-15 00:00:00

abstract：:Cells of Bradyrhizobium japonicum were grown in media containing either 1.0 mM or 0.5 muM phosphorus. In growth pouch experiments, infection of the primary root of soybean (Glycine max (L.) Merr.) by B. japonicum USDA 31, 110, and 142 was significantly delayed when P-limited cells were applied to the root. In a greenh...

journal_title：Applied and environmental microbiology

authors： Mullen MD,Israel DW,Wollum AG

更新日期：1988-10-01 00:00:00

abstract：:Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Sa...

journal_title：Applied and environmental microbiology

authors： Kim HJ,Park SH,Kim HY

更新日期：2006-09-01 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:Four bacteria, Bacillus cereus, B. subtilis, Escherichia coli, and Pseudomonas aeruginosa, were examined for the ability to remove Ag+, Cd2+, Cu2+, and La3+ from solution by batch equilibration methods. Cd and Cu sorption over the concentration range 0.001 to 1 mM was described by Freundlich isotherms. At 1 mM concent...

journal_title：Applied and environmental microbiology

authors： Mullen MD,Wolf DC,Ferris FG,Beveridge TJ,Flemming CA,Bailey GW

更新日期：1989-12-01 00:00:00

abstract：:Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. The objective of this study was to characterize a collection of 437 Salmonella enterica isolates from different ani...

journal_title：Applied and environmental microbiology

authors： Lindsey RL,Fedorka-Cray PJ,Frye JG,Meinersmann RJ

更新日期：2009-04-01 00:00:00

abstract：:Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purif...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Déziel E,Villemur R

更新日期：2010-11-01 00:00:00

abstract：:The widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. Few microbial isolates capable of transforming substituted s-triazines have been identified. Rhodococcus corallinus NRRL B-15444 has pre...

journal_title：Applied and environmental microbiology

authors： Mulbry WW

更新日期：1994-02-01 00:00:00