Disparate independent genetic events disrupt the secondary metabolism gene perA in certain symbiotic Epichloë species.

abstract：:This study describes the optimization of PCR parameters and testing of a wide number of microbial species to establish a highly specific and sensitive PCR-based method of detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 strain in pure cultures and seeded waters from the Gulf of Mexico (gulf water). ...

journal_title：Applied and environmental microbiology

authors： Myers ML,Panicker G,Bej AK

更新日期：2003-04-01 00:00:00

abstract：:The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

journal_title：Applied and environmental microbiology

authors： Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

更新日期：1993-05-01 00:00:00

abstract：:The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presen...

journal_title：Applied and environmental microbiology

authors： Valle J,Vadillo S,Piriz S,Gomez-Lucia E

更新日期：1991-03-01 00:00:00

abstract：:Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

journal_title：Applied and environmental microbiology

authors： Gelsomino R,Vancanneyt M,Cogan TM,Swings J

更新日期：2003-01-01 00:00:00

abstract：:Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...

journal_title：Applied and environmental microbiology

authors： Simonson J,Siebeling RJ

更新日期：1986-12-01 00:00:00

abstract：:Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

journal_title：Applied and environmental microbiology

authors： Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

更新日期：1999-11-01 00:00:00

abstract：:We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase...

journal_title：Applied and environmental microbiology

authors： Graziani S,Vasnier C,Daboussi MJ

更新日期：2004-05-01 00:00:00

abstract：:The effects of limiting concentrations of ammonium on the metabolic activity of Nitrosomonas europaea, an obligate ammonia-oxidizing soil bacterium, were investigated. Cells were harvested during late logarithmic growth and were incubated for 24 h in growth medium containing 0, 15, or 50 mM ammonium. The changes in ni...

journal_title：Applied and environmental microbiology

authors： Stein LY,Arp DJ

更新日期：1998-04-01 00:00:00

abstract：:Lactococcus lactis is a prokaryotic microorganism with great importance as a culture starter and has become the model species among the lactic acid bacteria. The long and safe history of use of L. lactis in dairy fermentations has resulted in the classification of this species as GRAS (General Regarded As Safe) or QPS...

journal_title：Applied and environmental microbiology

authors： Ladero V,Rattray FP,Mayo B,Martín MC,Fernández M,Alvarez MA

更新日期：2011-09-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turno...

journal_title：Applied and environmental microbiology

authors： Gärtner K,Lechno-Yossef S,Cornish AJ,Wolk CP,Hegg EL

更新日期：2012-12-01 00:00:00

abstract：:A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of...

journal_title：Applied and environmental microbiology

authors： Heipieper HJ,Diefenbach R,Keweloh H

更新日期：1992-06-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:Four pure cultures of denitrifying bacteria, which had previously been isolated on defined alkylbenzenes, were capable of anaerobic growth with crude oil as the only source of organic substrates. Chemical analyses after growth revealed that the known growth substrates toluene, ethylbenzene, and m-xylene were selective...

journal_title：Applied and environmental microbiology

authors： Rabus R,Widdel F

更新日期：1996-04-01 00:00:00

abstract：:Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking wat...

journal_title：Applied and environmental microbiology

authors： Delille A,Quilès F,Humbert F

更新日期：2007-09-01 00:00:00

abstract：:Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...

journal_title：Applied and environmental microbiology

authors： Vishnivetskaya TA,Kathariou S

更新日期：2005-11-01 00:00:00

abstract：:A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is det...

journal_title：Applied and environmental microbiology

authors： Hirsh DC,Martin LD

更新日期：1983-01-01 00:00:00

abstract：:L-threonine can be made by the amino acid-producing bacterium Corynebacterium glutamicum. However, in the course of this process, some of the L-threonine is degraded to glycine. We detected an aldole cleavage activity of L-threonine in crude extracts with an activity of 2.2 nmol min(-1) (mg of protein)(-1). In order t...

journal_title：Applied and environmental microbiology

authors： Simic P,Willuhn J,Sahm H,Eggeling L

更新日期：2002-07-01 00:00:00

abstract：:n-Alkanes are ubiquitous in nature and are widely used by microorganisms as carbon sources. Alkane hydroxylation by alkane monooxygenases is a critical step in the aerobic biodegradation of n-alkanes, which plays important roles in natural alkane attenuation and is used in industrial and environmental applications. Th...

journal_title：Applied and environmental microbiology

authors： Liang JL,Gao Y,He Z,Nie Y,Wang M,JiangYang JH,Zhang XC,Shu WS,Wu XL

更新日期：2017-10-17 00:00:00

abstract：:Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

journal_title：Applied and environmental microbiology

authors： Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

更新日期：1990-04-01 00:00:00

abstract：:Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-07-01 00:00:00

abstract：:Xanthobacter flavus 14p1 was isolated from sludge of the river Mulde by selective enrichment with 1,4-dichlorobenzene as the sole source of carbon and energy. The bacterium did not use other aromatic or chloroaromatic compounds as growth substrates. During growth on 1,4-dichlorobenzene, stoichiometric amounts of chlor...

journal_title：Applied and environmental microbiology

authors： Spiess E,Sommer C,Görisch H

更新日期：1995-11-01 00:00:00

abstract：:We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular po...

journal_title：Applied and environmental microbiology

authors： Suárez N,Fraguas LF,Texeira E,Massaldi H,Batista-Viera F,Ferreira F

更新日期：2001-02-01 00:00:00

abstract：:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...

journal_title：Applied and environmental microbiology

authors： Davidson MS,Roy P,Summers AO

更新日期：1985-06-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

journal_title：Applied and environmental microbiology

authors： Arthurs CE,Lloyd D

更新日期：1999-08-01 00:00:00

abstract：:Hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. Although these systems are common, little is known about the microbial populations that inhabit them or how...

journal_title：Applied and environmental microbiology

authors： McGarvey JA,Miller WG,Sanchez S,Stanker L

更新日期：2004-07-01 00:00:00

abstract：:A colorimetric assay for NADPH-dependent, mercuric ion-specific oxidoreductase activity was developed to facilitate the investigation of mercuric reductase gene expression in polluted aquatic ecosystems. Protein molecules extracted directly from unseeded freshwater and samples seeded with Pseudomonas aeruginosa PU21 (...

journal_title：Applied and environmental microbiology

authors： Ogunseitan OA

更新日期：1998-02-01 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

journal_title：Applied and environmental microbiology

authors： Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

更新日期：2005-08-01 00:00:00