Transpositional Mutagenesis of Thiobacillus novellus and Thiobacillus versutus.

Abstract:

:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for elemental sulfur (S), thiosulfate (S(2)O(3)), and tetrathionate (S(4)O(6)) in mutants of the latter type were reduced relative to those of the nonmutant control strain. Incorporation of labeled bicarbonate (HCO(3)) was also significantly impaired. Although suicide vehicles were not useful for the introduction of transposons into T. novellus, this method was effective for the Tn1721-induced mutagenesis of Thiobacillus versutus. Tn1721 insertions resulted in the loss of the natural resistance of T. versutus to arsenate and gentamicin and in auxotrophies for isoleucine-valine, arginine, phenylalanine, valine, and panthothenate. Transpositional mutagenesis by either method should prove to be a useful tool for further study of these and other members of the genus Thiobacillus.

journal_name

Appl Environ Microbiol

authors

Davidson MS,Roy P,Summers AO

doi

10.1128/AEM.49.6.1436-1441.1985

keywords:

subject

Has Abstract

pub_date

1985-06-01 00:00:00

pages

1436-41

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

49

pub_type

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