In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes.

abstract：:Sulfate-reducing bacteria (SRB) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. Although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to SRB, li...

journal_title：Applied and environmental microbiology

authors： Ekstrom EB,Morel FM,Benoit JM

更新日期：2003-09-01 00:00:00

abstract：:The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several differe...

journal_title：Applied and environmental microbiology

authors： Orphan VJ,Hinrichs KU,Ussler W 3rd,Paull CK,Taylor LT,Sylva SP,Hayes JM,Delong EF

更新日期：2001-04-01 00:00:00

abstract：:Anaerobic ammonium-oxidizing (anammox) bacteria have been recognized as an important sink for fixed nitrogen and are detected in many natural environments. However, their presence in terrestrial ecosystems has long been overlooked, and their contribution to the nitrogen cycling in natural and agricultural soils is cur...

journal_title：Applied and environmental microbiology

authors： Hu BL,Rush D,van der Biezen E,Zheng P,van Mullekom M,Schouten S,Sinninghe Damsté JS,Smolders AJ,Jetten MS,Kartal B

更新日期：2011-02-01 00:00:00

abstract：:Organic matter in sewage, soil, and aquatic systems may enhance or inhibit the infectivity of viruses associated with particulates (e.g., clay minerals, sediments). The purpose of this investigation was to identify the mechanisms whereby organic matter, in the form of defined proteins, affects the adsorption of reovir...

journal_title：Applied and environmental microbiology

authors： Lipson SM,Stotzky G

更新日期：1984-09-01 00:00:00

abstract：:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic...

journal_title：Applied and environmental microbiology

authors： Rabus R,Nordhaus R,Ludwig W,Widdel F

更新日期：1993-05-01 00:00:00

abstract：:The organization and species composition of bacterial trophic groups associated with lactose biomethanation were investigated in a whey-processing chemostat by enumeration, isolation, and general characterization studies. The bacteria were spatially organized as free-living forms and as self-immobilized forms appearin...

journal_title：Applied and environmental microbiology

authors： Chartrain M,Zeikus JG

更新日期：1986-01-01 00:00:00

abstract：:Borrelia spirochetes in bird-feeding ticks were studied in the Czech Republic. During the postbreeding period (July to September 2005), 1,080 passerine birds infested by 2,240 Ixodes ricinus subadult ticks were examined. Borrelia garinii was detected in 22.2% of the ticks, Borrelia valaisiana was detected in 12.8% of ...

journal_title：Applied and environmental microbiology

authors： Dubska L,Literak I,Kocianova E,Taragelova V,Sychra O

更新日期：2009-02-01 00:00:00

abstract：:We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

journal_title：Applied and environmental microbiology

authors： Liu J,Xie Z,Merritt J,Qi F

更新日期：2012-05-01 00:00:00

abstract：:We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuat...

journal_title：Applied and environmental microbiology

authors： Willyerd KL,Kemp AM,Dawe AL

更新日期：2009-08-01 00:00:00

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00

abstract：:Methanobacterium espanolae, an acidiphilic methanogen, required acetate for maximal growth on H(2)-CO(2). In the presence of 5 to 15 mM acetate, at a growth pH of 5.5, the mu(max) was 0.05 h. M. espanolae consumed 12.3 mM acetate during 96 h of incubation at 35 degrees C with shaking at 100 rpm. At initial acetate lev...

journal_title：Applied and environmental microbiology

authors： Patel GB,Sprott D,Ekiel I

更新日期：1993-04-01 00:00:00

abstract：:The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both...

journal_title：Applied and environmental microbiology

authors： Li B,Nagalla SR,Renganathan V

更新日期：1997-02-01 00:00:00

abstract：:A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup...

journal_title：Applied and environmental microbiology

authors： Rousseau DM,Slegers GA,Van Peteghem CH

更新日期：1985-08-01 00:00:00

abstract：:Compost amendment and inoculations with specific microorganisms are fundamentally different soil treatment methods, commonly used in agriculture for the improvement of plant growth and health. Although distinct, both methods affect the rhizosphere and the plant roots. In the present study we used a 16S rRNA gene appro...

journal_title：Applied and environmental microbiology

authors： Ofek M,Hadar Y,Minz D

更新日期：2009-10-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from ...

journal_title：Applied and environmental microbiology

authors： Kunjapur AM,Prather KL

更新日期：2015-03-01 00:00:00

abstract：:Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

journal_title：Applied and environmental microbiology

authors： Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

更新日期：2002-09-01 00:00:00

abstract：:The products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of Pseudomonas fluorescens Pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. Five analogs of rhizoxin, a 16-member macrolide w...

journal_title：Applied and environmental microbiology

authors： Loper JE,Henkels MD,Shaffer BT,Valeriote FA,Gross H

更新日期：2008-05-01 00:00:00

abstract：:In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the Bacteroidales markers wa...

journal_title：Applied and environmental microbiology

authors： Gourmelon M,Caprais MP,Ségura R,Le Mennec C,Lozach S,Piriou JY,Rincé A

更新日期：2007-08-01 00:00:00

abstract：:The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

journal_title：Applied and environmental microbiology

authors： Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

更新日期：2018-08-31 00:00:00

abstract：:Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We...

journal_title：Applied and environmental microbiology

authors： Matsuda M,Kaneko A,Stanley T,Millar BC,Miyajima M,Murphy PG,Moore JE

更新日期：2003-06-01 00:00:00

abstract：:Time series incubations were conducted to provide estimates for the size selectivities and rates of protistan grazing that may be occurring in a sandy, contaminated aquifer. The experiments involved four size classes of fluorescently labeled groundwater bacteria (FLB) and 2- to 3-microns-long nanoflagellates, primaril...

journal_title：Applied and environmental microbiology

authors： Kinner NE,Harvey RW,Blakeslee K,Novarino G,Meeker LD

更新日期：1998-02-01 00:00:00

abstract：:Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

journal_title：Applied and environmental microbiology

authors： Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

更新日期：1988-12-01 00:00:00

abstract：:Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fe...

journal_title：Applied and environmental microbiology

authors： Fein JE,Zawadzki BC,Lawford HG,Lawford GR

更新日期：1983-06-01 00:00:00

abstract：:Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to approximately 60 mM glutathione when this compound was added to the medium. S...

journal_title：Applied and environmental microbiology

authors： Li Y,Hugenholtz J,Abee T,Molenaar D

更新日期：2003-10-01 00:00:00

abstract：:Ruminal fluid from sheep fed hay plus concentrate diets containing 1.8% urea, 6% casein, or 6% egg albumin had proteolytic activities of 4.12, 3.02, or 4.00 mg of [14C]casein hydrolyzed ml-1 h-1, respectively. Dietary albumin had no effect on the rate of albumin breakdown relative to that of casein (0.06). Greater num...

journal_title：Applied and environmental microbiology

authors： Wallace RJ,Broderick GA,Brammall ML

更新日期：1987-04-01 00:00:00

abstract：:A membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of Aeromonas spp. in drinking water. The use of anaerobic incubation improved the detection of Aeromonas spp. by reducing the growth of nonaeromonads. The confirmation rate of presumptive...

journal_title：Applied and environmental microbiology

authors： Cunliffe DA,Adcock P

更新日期：1989-09-01 00:00:00

abstract：:Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46...

journal_title：Applied and environmental microbiology

authors： Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

更新日期：2009-09-01 00:00:00