Abstract:
:A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup method, the solvent partition method, and the Extrelut 3 column cleanup method) were compared.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Rousseau DM,Slegers GA,Van Peteghem CHdoi
10.1128/AEM.50.2.529-531.1985subject
Has Abstractpub_date
1985-08-01 00:00:00pages
529-31issue
2eissn
0099-2240issn
1098-5336journal_volume
50pub_type
杂志文章abstract::Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4272-4278.2000
更新日期:2000-10-01 00:00:00
abstract::Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02671-10
更新日期:2011-04-01 00:00:00
abstract::The presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. Several aspects of the biology of the interaction between these bacteria and alfalfa (Medicago sativa) seedlings are addressed here. A collection of enteric bacteria associated with alf...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.3.1783-1790.2003
更新日期:2003-03-01 00:00:00
abstract::High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.5.1723-1727.1996
更新日期:1996-05-01 00:00:00
abstract::A number of Micromonospora strains isolated from the water column, sediment, and cellulose baits placed in freshwater lakes were shown to be able to degrade cellulose in lake water without any addition of nutrients. A selective isolation method was also developed to demonstrate that CFU arose from both spores and hyph...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01092-08
更新日期:2008-11-01 00:00:00
abstract::Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.11.5017-5024.2001
更新日期:2001-11-01 00:00:00
abstract::The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01866-10
更新日期:2011-04-01 00:00:00
abstract::Human noroviruses (HuNoVs) are the leading cause of food-borne illness, accounting for 58% of U.S. cases. Because HuNoVs are unculturable, surrogates are needed to investigate transmission routes and evaluate disinfection methods. However, the current surrogates, feline calicivirus (FCV) and murine NoV (MNV), are less...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06600-11
更新日期:2012-06-01 00:00:00
abstract::Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding A...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01616-10
更新日期:2011-04-01 00:00:00
abstract::O-antigen biosynthetic (wbf) regions for Vibrio cholerae serogroups O5, O8, and O108 were isolated and sequenced. Sequences were compared to those of other published V. cholerae O-antigen regions. These wbf regions showed a high degree of heterogeneity both in gene content and in gene order. Genes identified frequentl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01663-10
更新日期:2011-04-01 00:00:00
abstract::In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly c...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2717-2722.1992
更新日期:1992-09-01 00:00:00
abstract::Cultures of rumen bacteria can be stored at -20 degrees C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.2.499-501.1982
更新日期:1982-08-01 00:00:00
abstract::Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are highly motile Gram-negative predatory bacteria with the potential of being used as biocontrol agents or living antibiotics. It was suggested previously that sugar-binding proteins play a role in M. aeruginosavorus and B. bacteriovorus host specificity and p...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02565-10
更新日期:2011-04-01 00:00:00
abstract::Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.5.2529-2534.2002
更新日期:2002-05-01 00:00:00
abstract::To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-defi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.3016-3023.2000
更新日期:2000-07-01 00:00:00
abstract::Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.2.288-296.1980
更新日期:1980-02-01 00:00:00
abstract::Desulfitobacterium frappieri PCP-1 was induced for ortho- and para-dechlorinating activities by different chlorophenols. Dehalogenation rates ranging from 25 to 1,158 nmol/min/mg of cell protein were observed according to the chlorophenol tested and the position of the chlorine removed. D. frappieri shows a broad subs...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.11.4603-4606.1998
更新日期:1998-11-01 00:00:00
abstract::Streptococcus pyogenes is a major cause of necrotizing fasciitis, a life-threatening subcutaneous soft-tissue infection. At the host infection site, the local environment and interactions between the host and bacteria have effects on bacterial gene expression profiles, while the gene expression pattern of S. pyogenes ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01428-19
更新日期:2019-10-16 00:00:00
abstract::The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.5.1667-1673.1997
更新日期:1997-05-01 00:00:00
abstract::An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00092-18
更新日期:2018-05-17 00:00:00
abstract::Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.4.1448-1451.1996
更新日期:1996-04-01 00:00:00
abstract::Environmental bacteria persist in various habitats, yet little is known about the genes that contribute to growth and survival in their respective ecological niches. Signature-tagged mutagenesis (STM) of Shewanella oneidensis MR-1 coupled with a screen involving incubations of mutant strains in anoxic aquifer sediment...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01086-06
更新日期:2007-01-01 00:00:00
abstract::Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experience...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.5.1690-1692.1994
更新日期:1994-05-01 00:00:00
abstract::Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2771-2775.1992
更新日期:1992-09-01 00:00:00
abstract::Fomites are known to play a role in the transmission of pathogens. Quantitative analysis of the parameters that affect sample recovery efficiency (SRE) at the limit of detection of viruses on fomites will aid in improving quantitative microbial risk assessment (QMRA) and infection control. The variability in SRE as a ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01370-12
更新日期:2012-11-01 00:00:00
abstract::β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00318-19
更新日期:2019-07-18 00:00:00
abstract::Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01536-18
更新日期:2018-10-30 00:00:00
abstract::Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.31.2.313-315.1976
更新日期:1976-02-01 00:00:00
abstract::Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.12.3107-3112.1988
更新日期:1988-12-01 00:00:00
abstract::A vineyard isolate of the yeast Saccharomyces cerevisiae, UCD932, was identified as a strain producing little or no detectable hydrogen sulfide during wine fermentation. Genetic analysis revealed that this trait segregated as a single genetic determinant. The gene also conferred a white colony phenotype on BiGGY agar ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01666-10
更新日期:2010-12-01 00:00:00