Studies on the production of fungal peroxidases in Aspergillus niger.

abstract：:Isolation and cultivation are a crucial step in elucidating the physiology, biogeochemistry, and ecosystem role of microorganisms. Many abundant marine bacteria, including the widespread Roseobacter clade-affiliated (RCA) cluster group, have not been cultured with traditional methods. Using novel techniques of coculti...

journal_title：Applied and environmental microbiology

authors： Mayali X,Franks PJ,Azam F

更新日期：2008-05-01 00:00:00

abstract：:A novel halophilic and metal-reducing bacterium, Orenia metallireducens strain Z6, was isolated from briny groundwater extracted from a 2.02 km-deep borehole in the Illinois Basin, IL. This organism shared 96% 16S rRNA gene similarity with Orenia marismortui but demonstrated physiological properties previously unknown...

journal_title：Applied and environmental microbiology

authors： Dong Y,Sanford RA,Boyanov MI,Kemner KM,Flynn TM,O'Loughlin EJ,Chang YJ,Locke RA Jr,Weber JR,Egan SM,Mackie RI,Cann I,Fouke BW

更新日期：2016-10-14 00:00:00

abstract：:An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent homogeneity from Escherichia coli K-12. The enzyme catalyzes the trans...

journal_title：Applied and environmental microbiology

authors： Schulz A,Taggeselle P,Tripier D,Bartsch K

更新日期：1990-01-01 00:00:00

abstract：:This study presents population analyses of microbial communities inhabiting a site of extreme acid mine drainage (AMD) production. The site is the inactive underground Richmond mine at Iron Mountain, Calif., where the weathering of a massive sulfide ore body (mostly pyrite) produces solutions with pHs of approximately...

journal_title：Applied and environmental microbiology

authors： Bond PL,Druschel GK,Banfield JF

更新日期：2000-11-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:Salmonella typhimurium accumulates glutamate in response to osmotic stress. Cells in aerobic exponential growth have an intracellular pool of approximately 125 nmol of glutamate mg of protein-1. When cells were grown in minimal medium with 500 mM NaCl, KCl, or sucrose, 290 to 430 nmol of glutamate was found to accumul...

journal_title：Applied and environmental microbiology

authors： Botsford JL,Alvarez M,Hernandez R,Nichols R

更新日期：1994-07-01 00:00:00

abstract：:Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

journal_title：Applied and environmental microbiology

authors： Ziołecki A,Wojciechowicz M

更新日期：1980-04-01 00:00:00

abstract：:Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

journal_title：Applied and environmental microbiology

authors： Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

更新日期：2012-01-01 00:00:00

abstract：:"Viili," a fermented milk product, has a firm but viscous consistency. It is produced with traditional mesophilic mixed-strain starters, which have various stabilities in dairy practice. Thirteen morphologically different types of phages were found in 90 viili samples studied by electron microscopy. Ten of the phage t...

journal_title：Applied and environmental microbiology

authors： Saxelin ML,Nurmiaho-Lassila EL,Meriläinen VT,Forsén RI

更新日期：1986-10-01 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compa...

journal_title：Applied and environmental microbiology

authors： Bosch T,Verkade E,van Luit M,Landman F,Kluytmans J,Schouls LM

更新日期：2015-01-01 00:00:00

abstract：:The survival of bacteria exposed to toxic compounds is a multifactorial phenomenon, involving well-known molecular mechanisms of resistance but also less-well-understood mechanisms of tolerance that need to be clarified. In particular, the contribution of biofilm formation to survival in the presence of toxic compound...

journal_title：Applied and environmental microbiology

authors： Perrin C,Briandet R,Jubelin G,Lejeune P,Mandrand-Berthelot MA,Rodrigue A,Dorel C

更新日期：2009-03-01 00:00:00

abstract：:The oil-degrading Arthrobacter sp. RAG-1 produced an extracellular nondialyzable emulsifying agent when grown on hexadecane, ethanol, or acetate medium. The emulsifier was prepared by two procedures: (i) heptane extraction of the cell-free culture medium and (ii) precipitation with ammonium sulfate. A convenient assay...

journal_title：Applied and environmental microbiology

authors： Rosenberg E,Zuckerberg A,Rubinovitz C,Gutnick DL

更新日期：1979-03-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:The prevalence of Escherichia coli O157 associated with feedlot cattle in Saskatchewan was determined in a 10-month longitudinal study (3 feedlots) and a point prevalence study (20 feedlots). The prevalence of E. coli O157 at the three different sites in the horizontal study varied from 2.5 to 45%. The point prevalenc...

journal_title：Applied and environmental microbiology

authors： Vidovic S,Korber DR

更新日期：2006-06-01 00:00:00

abstract：:Wieners and sausages were prepared which contained the most heat-tolerant representative of the Mycobacterium avium-Mycobacterium intracellulare complex we were able to obtain. They also were prepared with infected tissues obtained from tuberculous swine. Processing conditions were as varied as possible. Neither incor...

journal_title：Applied and environmental microbiology

authors： Merkal RS,Crawford JA,Whipple DL

更新日期：1979-11-01 00:00:00

abstract：:Three common food pathogenic microorganisms were exposed to treatments simulating those used in food processing. Treated cell suspensions were then analyzed for reduction in growth by plate counting. Flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) were carried out on treated cells stained for membr...

journal_title：Applied and environmental microbiology

authors： Kennedy D,Cronin UP,Wilkinson MG

更新日期：2011-07-01 00:00:00

abstract：:Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. (sup13)C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and to probe citrate or pyruvate utilization, noninvasively, in living cell suspensions. The utilization of isotopical...

journal_title：Applied and environmental microbiology

authors： Ramos A,Lolkema JS,Konings WN,Santos H

更新日期：1995-04-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses. The core, which extended 58.1 m below the seafloor, was composed of pelagic clays with several volcanic ash layers contai...

journal_title：Applied and environmental microbiology

authors： Inagaki F,Suzuki M,Takai K,Oida H,Sakamoto T,Aoki K,Nealson KH,Horikoshi K

更新日期：2003-12-01 00:00:00

abstract：:We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture. ...

journal_title：Applied and environmental microbiology

authors： Hirata Y,Minami J,Koyama M,Matsushita O,Katayama S,Jin F,Maeta H,Okabe A

更新日期：1995-11-01 00:00:00

abstract：:A model conditional-suicide system to control genetically engineered microorganisms able to degrade substituted benzoates is reported. The system is based on two elements. One element consists of a fusion between the promoter of the Pseudomonas putida TOL plasmid-encoded meta-cleavage pathway operon (P(m)) and the lac...

journal_title：Applied and environmental microbiology

authors： Contreras A,Molin S,Ramos JL

更新日期：1991-05-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：UNLABELLED:Avian and possum fecal droppings may negatively impact roof-harvested rainwater (RHRW) water quality due to the presence of zoonotic pathogens. This study was aimed at evaluating the performance characteristics of a possum feces-associated (PSM) marker by screening 210 fecal and wastewater samples from possu...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Hamilton KA,Gyawali P,Toze S,Haas CN

更新日期：2016-06-30 00:00:00

abstract：:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance. Therefore, benzene metabolism was investigated in Geobacter metallireducens, the only genetically tractable organism known to anaerobically degrade benzene. Trace amounts (<0.5 μM) of phenol accumulated in cult...

journal_title：Applied and environmental microbiology

authors： Zhang T,Tremblay PL,Chaurasia AK,Smith JA,Bain TS,Lovley DR

更新日期：2013-12-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the ...

journal_title：Applied and environmental microbiology

authors： Roggo C,Clerc EE,Hadadi N,Carraro N,Stocker R,van der Meer JR

更新日期：2018-08-31 00:00:00

abstract：:A toluene-degrading methanogenic consortium enriched from creosote-contaminated aquifer material was maintained on toluene as the sole carbon and energy source for 10 years. The species in the consortium were characterized by using a molecular approach. Total genomic DNA was isolated, and 16S rRNA genes were amplified...

journal_title：Applied and environmental microbiology

authors： Ficker M,Krastel K,Orlicky S,Edwards E

更新日期：1999-12-01 00:00:00