Abstract:
:Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. (sup13)C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and to probe citrate or pyruvate utilization, noninvasively, in living cell suspensions. The utilization of isotopically enriched substrates allowed us to account for the end products derived from the metabolism of endogenous reserves. The effect of environmental parameters, e.g., pH, gas atmosphere, and presence of malate, on the end products of citrate utilization was studied. Approximately 10% of the citrate supplied was converted to aspartate which remained inside the cells. A metabolic shift with pH was observed, with acetoin production being favored at pH 4, whereas lactate and acetate production increased significantly at higher pH values. The information obtained with NMR was complemented with studies on the relevant enzyme activities in the metabolic pathway of citrate breakdown. The intracellular pH of the cells was strongly dependent on the external pH; this result, together with the determination of the pH profile of the enzymic activities, allowed us to establish the basis for pH regulation; lactate dehydrogenase activity was optimal at pH 7, whereas the acetoin-forming enzymes displayed maximal activities below pH 5. Citrate utilization was also monitored in dilute cell suspensions for comparison with NMR experiments performed with dense suspensions.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ramos A,Lolkema JS,Konings WN,Santos Hdoi
10.1128/AEM.61.4.1303-1310.1995keywords:
subject
Has Abstractpub_date
1995-04-01 00:00:00pages
1303-10issue
4eissn
0099-2240issn
1098-5336journal_volume
61pub_type
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.62.2.469-472.1996
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.42.4.734-736.1981
更新日期:1981-10-01 00:00:00
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更新日期:1991-04-01 00:00:00
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doi:10.1128/AEM.60.10.3647-3652.1994
更新日期:1994-10-01 00:00:00
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doi:10.1128/AEM.65.8.3566-3574.1999
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pub_type: 杂志文章
doi:10.1128/AEM.53.7.1448-1453.1987
更新日期:1987-07-01 00:00:00
abstract::Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.2.288-296.1980
更新日期:1980-02-01 00:00:00
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更新日期:2003-01-01 00:00:00
abstract::Digital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema p...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01956-15
更新日期:2015-11-01 00:00:00