Molecular characterization of natural Erwinia pyrifoliae strains deficient in hypersensitive response.

abstract：:The membrane integrity of a cell is a well-accepted criterion for characterizing viable (active or inactive) cells and distinguishing them from damaged and membrane-compromised cells. This information is of major importance in studies of the function of microbial assemblages in natural environments, in order to assign...

journal_title：Applied and environmental microbiology

authors： Grégori G,Citterio S,Ghiani A,Labra M,Sgorbati S,Brown S,Denis M

更新日期：2001-10-01 00:00:00

abstract：:Trihalomethanes, carbon tetrachloride, 1,1,1-trichloroethane, 1,2-dibromoethane, chlorinated benzenes, ethylbenzene, and naphthalene at concentrations commonly found in surface and groundwater were incubated under anoxic conditions to study their transformability in the presence of denitrifying bacteria. None of the a...

journal_title：Applied and environmental microbiology

authors： Bouwer EJ,McCarty PL

更新日期：1983-04-01 00:00:00

abstract：:After treatment of a dispersed suspension of echovirus with HOCl, much of the lost plaque titer was restored if the treated virus was induced to aggregate by adjustment of the suspending medium to pH 4.5. This did not appear to be a repair of individual virions but rather a special kind of multiplicity-related increas...

journal_title：Applied and environmental microbiology

authors： Young DC,Sharp DG

更新日期：1979-04-01 00:00:00

abstract：:Intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. The quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microor...

journal_title：Applied and environmental microbiology

authors： Ercolini D,De Filippis F,La Storia A,Iacono M

更新日期：2012-11-01 00:00:00

abstract：:Multiple anthropogenic disturbances to bacterial diversity have been investigated in coastal ecosystems, in which temporal variability in the bacterioplankton community has been considered a ubiquitous process. However, far less is known about the temporal dynamics of a bacterioplankton community responding to polluti...

journal_title：Applied and environmental microbiology

authors： Wang K,Zhang D,Xiong J,Chen X,Zheng J,Hu C,Yang Y,Zhu J

更新日期：2015-01-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

journal_title：Applied and environmental microbiology

authors： Wilkinson TL,Koga R,Fukatsu T

更新日期：2007-02-01 00:00:00

abstract：:We have evaluated the effectiveness of API 20E, Biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (ERIC)-PCR to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant Obesumbacterium proteus. Of the five typing techniques, Biolog tes...

journal_title：Applied and environmental microbiology

authors： Prest AG,Hammond JR,Stewart GS

更新日期：1994-05-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00

abstract：:Strain SR, a monensin-sensitive, ammonia-producing ruminal bacterium, grew rapidly on arginine and lysine, but only if sodium was present. Arginine transport could be driven by either an electrical potential or a chemical gradient of sodium. Arginine was converted to ornithine, and it appeared that ornithine efflux cr...

journal_title：Applied and environmental microbiology

authors： Van Kessel JS,Russell JB

更新日期：1992-03-01 00:00:00

abstract：:One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum ...

journal_title：Applied and environmental microbiology

authors： Beylefeld A,Wambulawaye P,Bwala DG,Gouws JJ,Lukhele OM,Wandrag DBR,Abolnik C

更新日期：2018-10-17 00:00:00

abstract：:The efficiency of a system developed for the recovery of viruses contaminating large quantities of vegetables was investigated in the laboratory and tested in the field. Viruses seeded onto a number of leafy vegetables in the laboratory were eluted with a phosphate-buffered saline solution (pH 9.0). The eluate was cla...

journal_title：Applied and environmental microbiology

authors： Ward BK,Chenoweth CM,Irving LG

更新日期：1982-12-01 00:00:00

abstract：:Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

journal_title：Applied and environmental microbiology

authors： Arthurs CE,Lloyd D

更新日期：1999-08-01 00:00:00

abstract：:Forty-two samples taken from two landfills were monitored for CH(inf4) production and apparent steady-state H(inf2) concentration. The rates of methanogenesis in these samples ranged from below the detection limit to 1,900 (mu)mol kg (dry weight)(sup-1) day(sup-1), and the median steady-state hydrogen concentration wa...

journal_title：Applied and environmental microbiology

authors： Mormile MR,Gurijala KR,Robinson JA,McInerney MJ,Suflita JM

更新日期：1996-05-01 00:00:00

abstract：:Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relativ...

journal_title：Applied and environmental microbiology

authors： Król JE,Penrod JT,McCaslin H,Rogers LM,Yano H,Stancik AD,Dejonghe W,Brown CJ,Parales RE,Wuertz S,Top EM

更新日期：2012-02-01 00:00:00

abstract：:Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(...

journal_title：Applied and environmental microbiology

authors： Vernozy-Rozand C,Montet MP,Bertin Y,Trably F,Girardeau JP,Martin C,Livrelli V,Beutin L

更新日期：2004-04-01 00:00:00

abstract：:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron don...

journal_title：Applied and environmental microbiology

authors： Jiao Y,Kappler A,Croal LR,Newman DK

更新日期：2005-08-01 00:00:00

abstract：:Many Helicobacter pylori genetic studies would benefit from an ability to move DNA sequences easily between strains by transformation and homologous recombination, without needing to leave a conventional drug resistance determinant at the targeted locus. Presented here is a two-gene cassette that can be selected both ...

journal_title：Applied and environmental microbiology

authors： Dailidiene D,Dailide G,Kersulyte D,Berg DE

更新日期：2006-09-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00

abstract：:This study investigated the effect of a 30-cm covering of finished compost (FC) on survival of Escherichia coli O157:H7 and Salmonella spp. in active static and windrow composting systems. Feedstocks inoculated with E. coli O157:H7 (7.41 log CFU/g) and Salmonella (6.46 log CFU/g) were placed in biosentry tubes (7.5-cm...

journal_title：Applied and environmental microbiology

authors： Patel JR,Yossa I,Macarisin D,Millner P

更新日期：2015-03-01 00:00:00

abstract：:Stability of the plasmid pKK223-200 in Escherichia coli JM105 was studied for both free and immobilized cells during continuous culture. The relationship between plasmid copy number, xylanase activity, which was coded for by the plasmid, and growth rate and culture conditions involved complex interactions which determ...

journal_title：Applied and environmental microbiology

authors： de Taxis du Poët P,Arcand Y,Bernier R Jr,Barbotin JN,Thomas D

更新日期：1987-07-01 00:00:00

abstract：:The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic...

journal_title：Applied and environmental microbiology

authors： Yomantas YA,Tokmakova IL,Gorshkova NV,Abalakina EG,Kazakova SM,Gak ER,Mashko SV

更新日期：2010-01-01 00:00:00

abstract：:Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid co...

journal_title：Applied and environmental microbiology

authors： D'Andrea L,Pérez-Rodríguez FJ,Costafreda MI,Beguiristain N,Fuentes C,Aymerich T,Guix S,Bosch A,Pintó RM

更新日期：2014-10-01 00:00:00

abstract：:Some Listeria monocytogenes internalins are recognized as contributing to invasion of mammalian tissue culture cells. While PrfA is well established as a positive regulator of L. monocytogenes virulence gene expression, the stress-responsive sigma(B) has been recognized only recently as contributing to expression of v...

journal_title：Applied and environmental microbiology

authors： McGann P,Wiedmann M,Boor KJ

更新日期：2007-05-01 00:00:00

abstract：:The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...

journal_title：Applied and environmental microbiology

authors： Gupta KR,Kasetty S,Chatterji D

更新日期：2015-04-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:Because dihydrostreptomycin can remain as a slowly removed antibiotic residue in dairy animals and because of the need for more sensitive procedures with which to provide information concerning antibiotic residues in food products, procedures were developed in more sensitive assays of dihydrostreptomycin in milk and s...

journal_title：Applied and environmental microbiology

authors： Inglis JM,Katz SE

更新日期：1978-03-01 00:00:00

abstract：:A cup of coffee is the final product of a complex chain of operations. Wet postharvest processing of coffee is one of these operations, which involves a fermentation that inevitably has to be performed on-farm. During wet coffee processing, the interplay between microbial activities and endogenous bean metabolism resu...

journal_title：Applied and environmental microbiology

authors： Zhang SJ,De Bruyn F,Pothakos V,Torres J,Falconi C,Moccand C,Weckx S,De Vuyst L

更新日期：2019-03-06 00:00:00

abstract：:A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa mo...

journal_title：Applied and environmental microbiology

authors： Arif MI,Samin G,van Leeuwen JG,Oppentocht J,Janssen DB

更新日期：2012-09-01 00:00:00

abstract：:When carbon sources are changed, Saccharomyces cerevisiae transcriptional patterns drastically change. To identify genes whose transcription can be used to quantitatively measure sugar concentrations, we searched genomic expression databases for a set of genes that are highly induced during the diauxic shift, and we u...

journal_title：Applied and environmental microbiology

authors： Chambers P,Issaka A,Palecek SP

更新日期：2004-01-01 00:00:00