Kinetics, stereospecificity, and expression of the malolactic enzyme.

abstract：:Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified ...

journal_title：Applied and environmental microbiology

authors： Jackson CR,Harper JP,Willoughby D,Roden EE,Churchill PF

更新日期：1997-12-01 00:00:00

abstract：:Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

journal_title：Applied and environmental microbiology

authors： Gupta J,Pathak B,Sethi N,Vora VC

更新日期：1981-03-01 00:00:00

abstract：:POL-88, a mutant of the white-rot fungus Phanerochaete chrysosporium, was selected for diminished phenol-oxidizing enzyme activity. A wide variety of phenolic compounds were degraded by ligninolytic cultures of this mutant. With several o-diphenolic substrates, degradation intermediates were produced that had UV spect...

journal_title：Applied and environmental microbiology

authors： Leatham GF,Crawford RL,Kirk TK

更新日期：1983-07-01 00:00:00

abstract：:Microbial diversity of septic tank effluent (STE) and the biomat that is formed as a result of STE infiltration on soil were characterized by 16S rRNA gene sequence analysis. Results indicate that microbial communities are different within control soil, STE, and the biomat and that microbes found in STE are not found ...

journal_title：Applied and environmental microbiology

authors： Tomaras J,Sahl JW,Siegrist RL,Spear JR

更新日期：2009-05-01 00:00:00

abstract：:The control of promoter activity by oxygen availability appears to be an intriguing system for heterologous protein production. In fact, during cell growth in a bioreactor, an oxygen shortage is easily obtained simply by interrupting the air supply. The purpose of our work was to explore the possible use of hypoxic in...

journal_title：Applied and environmental microbiology

authors： Camattari A,Bianchi MM,Branduardi P,Porro D,Brambilla L

更新日期：2007-02-01 00:00:00

abstract：:Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...

journal_title：Applied and environmental microbiology

authors： Reissbrodt R,Heier H,Tschäpe H,Kingsley RA,Williams PH

更新日期：2000-09-01 00:00:00

abstract：:Oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. Steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (CHX) gluconate-containing mouthwash (CHXM) diluted to 0.06% (wt...

journal_title：Applied and environmental microbiology

authors： McBain AJ,Bartolo RG,Catrenich CE,Charbonneau D,Ledder RG,Gilbert P

更新日期：2003-08-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:A 5' nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose activity was modulated...

journal_title：Applied and environmental microbiology

authors： Norton DM,Batt CA

更新日期：1999-05-01 00:00:00

abstract：:Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

journal_title：Applied and environmental microbiology

authors： Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

更新日期：2001-09-01 00:00:00

abstract：:The intestinal contents of 59 Zairese freshwater fish were examined for the presence of potential human enteric pathogens. Edwardsiella tarda and Plesiomonas shigelloides were isolated from 57 and 59% of them, respectively. For both microorganisms there was a significant difference between the isolation rates from lak...

journal_title：Applied and environmental microbiology

authors： Van Damme LR,Vandepitte J

更新日期：1980-03-01 00:00:00

abstract：:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can ...

journal_title：Applied and environmental microbiology

authors： Haarman M,Knol J

更新日期：2006-04-01 00:00:00

abstract：:Bacteria able to perform dissimilatory nitrate reduction to ammonium were isolated from low-oxygen masses in the Baltic Sea. In liquid media enriched with NO(3) and incubated anaerobically, the NH(4)-producing isolates transformed 25 to 72% of the NO(3) to NH(4). ...

journal_title：Applied and environmental microbiology

authors： Samuelsson MO,Rönner U

更新日期：1982-11-01 00:00:00

abstract：:Fecal Candida pintolopesii population levels were found to be significantly affected by laparotomy-inoculation-Bollman apparatus restraint, laparotomy-inoculation, and a milk diet. Gastrectomized rats could not support yeast populations; in intact animals, yeast cells failed to colonize the gastrointestinal tract dist...

journal_title：Applied and environmental microbiology

authors： Artwohl J,McClain A,Cera L

更新日期：1988-10-01 00:00:00

abstract：:The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. ...

journal_title：Applied and environmental microbiology

authors： Jiang L,Liu X,Dong C,Huang Z,Cambon-Bonavita MA,Alain K,Gu L,Wang S,Shao Z

更新日期：2020-04-01 00:00:00

abstract：:Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize tr...

journal_title：Applied and environmental microbiology

authors： Carvalho AL,Cardoso FS,Bohn A,Neves AR,Santos H

更新日期：2011-06-01 00:00:00

abstract：:Hepatitis A virus (HAV) is a food-borne enteric virus responsible for outbreaks of hepatitis associated with shellfish consumption. The objectives of this study were to determine the thermal inactivation behavior of HAV in blue mussels, to compare the first-order and Weibull models to describe the data, to calculate A...

journal_title：Applied and environmental microbiology

authors： Bozkurt H,D'Souza DH,Davidson PM

更新日期：2014-05-01 00:00:00

abstract：:AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein...

journal_title：Applied and environmental microbiology

authors： Haaber J,Samson JE,Labrie SJ,Campanacci V,Cambillau C,Moineau S,Hammer K

更新日期：2010-11-01 00:00:00

abstract：:Flavonoids are 15-carbon plant secondary metabolites exuded in the rhizosphere that hosts several flavonoid-degrading bacteria. We studied flavonoid catabolism in a plant growth-promoting rhizobacterial strain of Pseudomonas by using a combination of biochemical and genetic approaches. Transposants carrying mini-Tn5gf...

journal_title：Applied and environmental microbiology

authors： Pillai BV,Swarup S

更新日期：2002-01-01 00:00:00

abstract：:Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

journal_title：Applied and environmental microbiology

authors： Keleti G,Sykora JL

更新日期：1982-01-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

journal_title：Applied and environmental microbiology

authors： Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

更新日期：2016-05-16 00:00:00

abstract：:Divercin V41 (DV41) is a class IIa bacteriocin produced by Carnobacterium divergens V41. This antilisterial peptide is homologous to pediocin PA-1 and contains two disulfide bonds. To establish the structure-activity relationships of this specific family of bacteriocin, chemical modifications and enzymatic hydrolysis ...

journal_title：Applied and environmental microbiology

authors： Bhugaloo-Vial P,Douliez JP,Moll D,Dousset X,Boyaval P,Marion D

更新日期：1999-07-01 00:00:00

abstract：:Enterocytozoon bieneusi is an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts of E. bieneusi based on their high prevalence rates and zoonotic genotypes in pi...

journal_title：Applied and environmental microbiology

authors： Zhao W,Zhang W,Yang F,Cao J,Liu H,Yang D,Shen Y,Liu A

更新日期：2014-06-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:While screening aerobic, heterotrophic marine bacteria for production of volatile organic compounds, we found that a group of isolates produced substantial amounts of acetone. Acetone production was confirmed by gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The m...

journal_title：Applied and environmental microbiology

authors： Nemecek-Marshall M,Wojciechowski C,Kuzma J,Silver GM,Fall R

更新日期：1995-01-01 00:00:00

abstract：:The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

journal_title：Applied and environmental microbiology

authors： Pottage T,Macken S,Giri K,Walker JT,Bennett AM

更新日期：2012-06-01 00:00:00

abstract：:The importance of viruses in aquatic ecosystem functioning has been widely described. However, few studies have examined tropical aquatic ecosystems. Here, we evaluated for the first time viruses and their relationship with other planktonic communities in an Amazonian freshwater ecosystem. Coupling between viruses and...

journal_title：Applied and environmental microbiology

authors： Barros N,Farjalla VF,Soares MC,Melo RC,Roland F

更新日期：2010-11-01 00:00:00

abstract：:Through genome-wide transcript analysis of a reference strain and two recombinant Saccharomyces cerevisiae strains with different rates of galactose uptake, we obtained information about the global transcriptional response to metabolic engineering of the GAL gene regulatory network. One of the recombinant strains over...

journal_title：Applied and environmental microbiology

authors： Bro C,Knudsen S,Regenberg B,Olsson L,Nielsen J

更新日期：2005-11-01 00:00:00