Low-temperature decontamination with hydrogen peroxide or chlorine dioxide for space applications.

abstract：:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pse...

journal_title：Applied and environmental microbiology

authors： Wutipraditkul N,Waditee R,Incharoensakdi A,Hibino T,Tanaka Y,Nakamura T,Shikata M,Takabe T,Takabe T

更新日期：2005-08-01 00:00:00

abstract：:AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein...

journal_title：Applied and environmental microbiology

authors： Haaber J,Samson JE,Labrie SJ,Campanacci V,Cambillau C,Moineau S,Hammer K

更新日期：2010-11-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correla...

journal_title：Applied and environmental microbiology

authors： Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P

更新日期：2008-05-01 00:00:00

abstract：:A mixture of a cell culture-adapted strain (C-486) of calf rotavirus and poliovirus type 1 (Sabin) was prepared in tryptose phosphate broth containing 0.1% uranine (physical tracer) and antifoam at a final concentration of 0.001%. By using a six-jet Collison nebulizer, the mixture was aerosolized into a 300-liter stai...

journal_title：Applied and environmental microbiology

authors： Ijaz MK,Sattar SA,Johnson-Lussenburg CM,Springthorpe VS

更新日期：1985-02-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:The hindguts of lower termites and Cryptocercus cockroaches are home to a distinct community of archaea, bacteria, and protists (primarily parabasalids and some oxymonads). Within a host species, the composition of these hindgut communities appears relatively stable, but the evolutionary and ecological factors structu...

journal_title：Applied and environmental microbiology

authors： Tai V,James ER,Nalepa CA,Scheffrahn RH,Perlman SJ,Keeling PJ

更新日期：2015-02-01 00:00:00

abstract：:Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less ...

journal_title：Applied and environmental microbiology

authors： Cole MA

更新日期：1977-02-01 00:00:00

abstract：:Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carr...

journal_title：Applied and environmental microbiology

authors： Deleo PC,Baveye P

更新日期：1996-12-01 00:00:00

abstract：:The microflora in strained rumen fluid did not methylate or volatilize 203Hg2+ at detectable rates. However, there was an exponential decay in the concentration of added CH3Hg+, which was attributed to demethylation. The major product of demethylation was metallic mercury (Hg0), and it was released as a volatile produ...

journal_title：Applied and environmental microbiology

authors： Kozak S,Forsberg CW

更新日期：1979-10-01 00:00:00

abstract：:Chitin is massively produced by freshwater plankton species as a structural element of their exoskeleton or cell wall. At the same time, chitin does not accumulate in the predominantly anoxic sediments, underlining its importance as carbon and nitrogen sources for sedimentary microorganisms. We studied chitin degradat...

journal_title：Applied and environmental microbiology

authors： Wörner S,Pester M

更新日期：2019-08-29 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:The variation in manure-amended soil survival capability among 18 Escherichia coli O157 strains (8 animal, 1 food, and 9 human isolates) was studied using a single sandy soil sample and a single sample of cattle manure as the inoculum carrier. The virulence profiles of E. coli O157 strains were characterized by detect...

journal_title：Applied and environmental microbiology

authors： Franz E,van Hoek AH,Bouw E,Aarts HJ

更新日期：2011-11-01 00:00:00

abstract：:Simulation of a heat process used in the terminal dry-heat decontamination of the Viking spacecraft is reported. Naturally occurring airborne bacterial spores were collected on Teflon ribbons in selected spacecraft assembly areas and subsequently subjected to dry heat. Thermal inactivation experiments were conducted a...

journal_title：Applied and environmental microbiology

authors： Puleo JR,Bergstrom SL,Peeler JT,Oxborrow GS

更新日期：1978-09-01 00:00:00

abstract：:Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of ...

journal_title：Applied and environmental microbiology

authors： Kreader CA

更新日期：1995-04-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:Bacteroides xylanolyticus X5-1 was grown in pure culture and in mixed culture with Methanospirillum hungatei JF-1 under xylose limitation in the chemostat. In the pure culture, ethanol, acetate, CO(2), and hydrogen were the products. In the mixed culture, acetate, CO(2), and presumably hydrogen were the only products ...

journal_title：Applied and environmental microbiology

authors： Biesterveld S,Zehnder AJ,Stams AJ

更新日期：1994-04-01 00:00:00

abstract：:Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detectable amounts of CH...

journal_title：Applied and environmental microbiology

authors： Harper DB,Buswell JA,Kennedy JT,Hamilton JT

更新日期：1990-11-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:The detection and enumeration of most cultivable human enteric viruses from water is possible if samples are first inoculated onto a suitable cell line such as MA-104 or BGM. Virus growth is then detected by an indirect immunoperoxidase method with human immune serum globulin as the source of antibody to most enteric ...

journal_title：Applied and environmental microbiology

authors： Payment P,Trudel M

更新日期：1985-11-01 00:00:00

abstract：:In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...

journal_title：Applied and environmental microbiology

authors： Bettarel Y,Sime-Ngando T,Amblard C,Laveran H

更新日期：2000-06-01 00:00:00

abstract：:Specific oxygen uptake rates [Q(02)] were highest when Candida 107 and Rhodotorula gracilis grew at their maximum dilution rates. In nitrogen-limited medium, which encourages lipid accumulation, Q(02) values of both yeasts decreased as the dilution rate was lowered. ...

journal_title：Applied and environmental microbiology

authors： Ratledge C,Hall MJ

更新日期：1977-08-01 00:00:00

abstract：:A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

journal_title：Applied and environmental microbiology

authors： Chung CC,Hwang SP,Chang J

更新日期：2005-12-01 00:00:00

abstract：:To ascertain whether position 131 of a mesophilic protease, subtilisin BPN', is a potential critical site for cold adaptation as screened by evolutionary engineering (S. Taguchi, A. Ozaki, and H. Momose, Appl. Environ. Microbiol. 64:492-495, 1998), a full set of subtilisin BPN' mutants with mutations at position 131 w...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Komada S,Momose H

更新日期：2000-04-01 00:00:00

abstract：:NAD-linked alcohol dehydrogenase activity was detected in cell-free crude extracts from various propane-grown bacteria. Two NAD-linked alcohol dehydrogenases, one which preferred primary alcohols (alcohol dehydrogenase I) and another which preferred secondary alcohols (alcohol dehydrogenase II), were found in propane-...

journal_title：Applied and environmental microbiology

authors： Hou CT,Patel RN,Laskin AI,Barist I,Barnabe N

更新日期：1983-07-01 00:00:00

abstract：:In this study, we used chemostat cultures to analyze the quantitative effects of the specific growth rate and respiration on the metabolism in Lactococcus lactis CHCC2862 and on the downstream robustness of cells after freezing or freeze-drying. Under anaerobic conditions, metabolism remained homofermentative, althoug...

journal_title：Applied and environmental microbiology

authors： Johanson A,Goel A,Olsson L,Franzén CJ

更新日期：2020-03-02 00:00:00

abstract：:Glycogen branching enzymes (GBE) or 1,4-alpha-glucan branching enzymes (EC 2.4.1.18) introduce alpha-1,6 branching points in alpha-glucans, e.g., glycogen. To identify structural features in GBEs that determine their branching pattern specificity, the Deinococcus geothermalis and Deinococcus radiodurans GBE (GBE(Dg) a...

journal_title：Applied and environmental microbiology

authors： Palomo M,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2009-03-01 00:00:00

abstract：:The development of high-throughput methods, such as the construction of 18S rRNA gene clone or pyrosequencing libraries, has allowed evaluation of ciliate community composition in hundreds of samples from the rumen and other intestinal habitats. However, several genera of mammalian intestinal ciliates have been descri...

journal_title：Applied and environmental microbiology

authors： Kittelmann S,Devente SR,Kirk MR,Seedorf H,Dehority BA,Janssen PH

更新日期：2015-04-01 00:00:00

abstract：:In the United States, the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths from April 2009 to April 2010. On 24 April 2009, the CDC posted interim guidance on infection control measures in health care settings explicitly for pH...

journal_title：Applied and environmental microbiology

authors： Coulliette AD,Perry KA,Edwards JR,Noble-Wang JA

更新日期：2013-04-01 00:00:00

abstract：:To longitudinally assess fecal shedding and animal-to-animal transmission of Clostridium difficile among finishing feedlot cattle as a risk for beef carcass contamination, we tested 186 ± 12 steers (mean ± standard deviation; 1,369 samples) in an experimental feedlot facility during the finishing period and at harvest...

journal_title：Applied and environmental microbiology

authors： Rodriguez-Palacios A,Pickworth C,Loerch S,LeJeune JT

更新日期：2011-05-01 00:00:00