Regulation of Product Formation in Bacteroides xylanolyticus X5-1 by Interspecies Electron Transfer.

Abstract:

:Bacteroides xylanolyticus X5-1 was grown in pure culture and in mixed culture with Methanospirillum hungatei JF-1 under xylose limitation in the chemostat. In the pure culture, ethanol, acetate, CO(2), and hydrogen were the products. In the mixed culture, acetate, CO(2), and presumably hydrogen were the only products formed by B. xylanolyticus X5-1. The biomass yield of B. xylanolyticus X5-1 increased because of cocultivation. In cell extracts of the pure culture, both NAD- and NADP-dependent acetaldehyde dehydrogenase and ethanol dehydrogenase activities were found. In cell extracts of the mixed culture, activities of these enzymes were not detected. Inhibition of methanogenesis in the mixed culture by the addition of bromoethanosulfonic acid (BES) resulted in an accumulation of H(2), ethanol, and formate. Immediately after the addition of BES, NAD-dependent acetaldehyde dehydrogenase and ethanol dehydrogenase activities were detected. After a short lag phase, a NADP-dependent ethanol dehydrogenase was also detectable. The induction of acetaldehyde dehydrogenase and ethanol dehydrogenase was inhibited by chloramphenicol, suggesting de novo synthesis of these enzymes. These results are consistent with a model in which the shift in product formation caused by interspecies electron transfer is regulated at the level of enzyme synthesis.

journal_name

Appl Environ Microbiol

authors

Biesterveld S,Zehnder AJ,Stams AJ

doi

10.1128/AEM.60.4.1347-1352.1994

keywords:

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

1347-52

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

60

pub_type

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