Current production and metal oxide reduction by Shewanella oneidensis MR-1 wild type and mutants.

abstract：:To ascertain whether position 131 of a mesophilic protease, subtilisin BPN', is a potential critical site for cold adaptation as screened by evolutionary engineering (S. Taguchi, A. Ozaki, and H. Momose, Appl. Environ. Microbiol. 64:492-495, 1998), a full set of subtilisin BPN' mutants with mutations at position 131 w...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Komada S,Momose H

更新日期：2000-04-01 00:00:00

abstract：:Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...

journal_title：Applied and environmental microbiology

authors： Alvarez B,Secades P,McBride MJ,Guijarro JA

更新日期：2004-01-01 00:00:00

abstract：:Methanogenic isolates did not consume hydrogen below partial pressures of 6.5 Pa. Thus, in contrast to a previous report, results from pure-culture studies do not invalidate the threshold model for methane production from hydrogen in sediments. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR

更新日期：1985-06-01 00:00:00

abstract：:Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...

journal_title：Applied and environmental microbiology

authors： Heinänen A,Kuparinen J

更新日期：1991-11-01 00:00:00

abstract：:Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isot...

journal_title：Applied and environmental microbiology

authors： Sun W,Sun X,Cupples AM

更新日期：2012-04-01 00:00:00

abstract：:The degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in Rhodococcus sp. strain TE1. Plasmid DNA libraries of Rhodococcus sp. strain TE1 were constructed in a Rhodococcus-Escherichia coli shu...

journal_title：Applied and environmental microbiology

authors： Shao ZQ,Behki R

更新日期：1995-05-01 00:00:00

abstract：:After treatment of a dispersed suspension of echovirus with HOCl, much of the lost plaque titer was restored if the treated virus was induced to aggregate by adjustment of the suspending medium to pH 4.5. This did not appear to be a repair of individual virions but rather a special kind of multiplicity-related increas...

journal_title：Applied and environmental microbiology

authors： Young DC,Sharp DG

更新日期：1979-04-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:We used a combination of stable isotope probing (SIP), gas chromatography-mass spectrometry-based respiration, isolation/cultivation, and quantitative PCR procedures to discover the identity and in situ growth of soil microorganisms that metabolize benzoic acid. We added [(13)C]benzoic acid or [(12)C]benzoic acid (100...

journal_title：Applied and environmental microbiology

authors： Pumphrey GM,Madsen EL

更新日期：2008-07-01 00:00:00

abstract：:Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

journal_title：Applied and environmental microbiology

authors： Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

更新日期：2018-11-15 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

journal_title：Applied and environmental microbiology

authors： Lakshmidevi G,Davidson BE,Hillier AJ

更新日期：1988-04-01 00:00:00

abstract：:Five genes encoding PhaP family proteins and one phaR gene have been identified in the genome of Burkholderia symbiont strain RPE75. PhaP proteins function as the surface proteins of polyhydroxyalkanoate (PHA) granules, and the PhaR protein acts as a negative regulator of PhaP biosynthesis. Recently, we characterized ...

journal_title：Applied and environmental microbiology

authors： Jang SH,Jang HA,Lee J,Kim JU,Lee SA,Park KE,Kim BH,Jo YH,Lee BL

更新日期：2017-05-17 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:In this work, a strain of anaerobic pentachlorophenol (PCP) degrader, Desulfitobacterium frappieri PCP-1, was used to augment a mixed bacterial community of an anaerobic upflow sludge bed reactor degrading PCP. To estimate the efficiency of augmentation, the population of PCP-1 in the reactor was enumerated by a compe...

journal_title：Applied and environmental microbiology

authors： Tartakovsky B,Levesque M,Dumortier R,Beaudet R,Guiot SR

更新日期：1999-10-01 00:00:00

abstract：:The mercury resistance (mer) operon of plasmid R100 was cloned onto various plasmid vectors to study the effect of mer gene amplification on the rate of Hg2+ reduction by Escherichia coli cells. The plasmids were maintained at copy numbers ranging from 3 to 140 copies per cell. The overall Hg2+ reduction rate of intac...

journal_title：Applied and environmental microbiology

authors： Philippidis GP,Malmberg LH,Hu WS,Schottel JL

更新日期：1991-12-01 00:00:00

abstract：:Despite the importance of human noroviruses (NoVs) in public health, little information concerning the effectiveness of ozone against NoVs is available. We determined the efficacy of ozone disinfection using murine norovirus (MNV) as a surrogate of human NoV. MNV in ozone demand-free buffer was exposed to a predetermi...

journal_title：Applied and environmental microbiology

authors： Lim MY,Kim JM,Lee JE,Ko G

更新日期：2010-02-01 00:00:00

abstract：:Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast e...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-10-01 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:Penicillium expansum isolated from meat and apples produced both patulin and citrinin. Toxin identity was confirmed by spectroscopic and physical methods. The mean lethal dose in chicken embryos was determined for toxins administered both singly and in various ratios. Data from simultaneous administration of mycotoxin...

journal_title：Applied and environmental microbiology

authors： Ciegler A,Vesonder RF,Jackson LK

更新日期：1977-04-01 00:00:00

abstract：:A 5' nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose activity was modulated...

journal_title：Applied and environmental microbiology

authors： Norton DM,Batt CA

更新日期：1999-05-01 00:00:00

abstract：:The minimal nutritional growth requirements were determined for strains Okra B and Iwanai E, which are representatives of groups I and II, respectively, of Clostridium botulinum. These type B and E strains differed considerably in their nutrient requirements. The organic growth factors required in high concentrations ...

journal_title：Applied and environmental microbiology

authors： Whitmer ME,Johnson EA

更新日期：1988-03-01 00:00:00

abstract：:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (b...

journal_title：Applied and environmental microbiology

authors： Ceragioli M,Mols M,Moezelaar R,Ghelardi E,Senesi S,Abee T

更新日期：2010-05-01 00:00:00

abstract：:Environmental contamination with compounds containing oxyanions of chlorine, such as perchlorate or chlorate [(per)chlorate] or chlorine dioxide, has been a constantly growing problem over the last 100 years. Although the fact that microbes reduce these compounds has been recognized for more than 50 years, only six or...

journal_title：Applied and environmental microbiology

authors： Coates JD,Michaelidou U,Bruce RA,O'Connor SM,Crespi JN,Achenbach LA

更新日期：1999-12-01 00:00:00

abstract：:To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

journal_title：Applied and environmental microbiology

authors： Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

更新日期：1990-05-01 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded i...

journal_title：Applied and environmental microbiology

authors： Kane SR,Beller HR,Legler TC,Koester CJ,Pinkart HC,Halden RU,Happel AM

更新日期：2001-12-01 00:00:00

abstract：:The anti-norovirus (anti-NoV) effect of grape seed extract (GSE) was examined by plaque assay for murine norovirus 1 (MNV-1), cell-binding reverse transcription-PCR for human NoV GII.4, and saliva-binding enzyme-linked immunosorbent assay for human NoV GII.4 P particles, with or without the presence of interfering sub...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,Zhang D,Xia M,Zhong W,Van Coillie E,Jiang X,Uyttendaele M

更新日期：2012-11-01 00:00:00

abstract：:We studied the physiological effect of the interconversion between the NAD(H) and NADP(H) coenzyme systems in recombinant Saccharomyces cerevisiae expressing the membrane-bound transhydrogenase from Escherichia coli. Our objective was to determine if the membrane-bound transhydrogenase could work in reoxidation of NAD...

journal_title：Applied and environmental microbiology

authors： Anderlund M,Nissen TL,Nielsen J,Villadsen J,Rydström J,Hahn-Hägerdal B,Kielland-Brandt MC

更新日期：1999-06-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00