Development of improved defined media for Clostridium botulinum serotypes A, B, and E.

abstract：:Inactivation of Bacillus cereus spores during cooling (10 degrees C/h) from 90 degrees C occurred in two phases. One phase occurred during cooling from 90 to 80 degrees C; the second occurred during cooling from 46 to 38 degrees C. In contrast, no inactivation occurred when spores were cooled from a maximum temperatur...

journal_title：Applied and environmental microbiology

authors： Johnson KM,Busta FF

更新日期：1984-04-01 00:00:00

abstract：:Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

journal_title：Applied and environmental microbiology

authors： Schesser K,Luder A,Henson JM

更新日期：1991-02-01 00:00:00

abstract：:Dextransucrase (DSR-S) from Leuconostoc mesenteroides NRRL B-512F is a glucosyltransferase that catalyzes synthesis of soluble dextran from sucrose. In the presence of efficient acceptor molecules, such as maltose, the reaction pathway is shifted toward glucooligosaccharide synthesis. Like glucosyltransferases from or...

journal_title：Applied and environmental microbiology

authors： Monchois V,Reverte A,Remaud-Simeon M,Monsan P,Willemot RM

更新日期：1998-05-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:Many pathogenic yeast species are asexual and therefore not involved in intra- or interspecies mating. However, high-frequency transfer of plasmid DNA was observed when pathogenic and food-borne yeasts were grown together. This property could play a crucial role in the spread of virulence and drug resistance factors a...

journal_title：Applied and environmental microbiology

authors： Mentel M,Spírek M,Jørck-Ramberg D,Piskur J

更新日期：2006-07-01 00:00:00

abstract：:Carnobacterium maltaromaticum UAL307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous Listeria species. Three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry an...

journal_title：Applied and environmental microbiology

authors： Martin-Visscher LA,van Belkum MJ,Garneau-Tsodikova S,Whittal RM,Zheng J,McMullen LM,Vederas JC

更新日期：2008-08-01 00:00:00

abstract：:Plasmid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda pL and lambda pR. Thermoregulated ex...

journal_title：Applied and environmental microbiology

authors： Wipat A,Wellington EM,Saunders VA

更新日期：1991-11-01 00:00:00

abstract：:The metal-reducing bacterium Shewanella oneidensis MR-1 displays remarkable anaerobic respiratory plasticity, which is reflected in the extensive number of electron transport components encoded in its genome. In these studies, several cell components required for the reduction of vanadium(V) were determined. V(V) redu...

journal_title：Applied and environmental microbiology

authors： Myers JM,Antholine WE,Myers CR

更新日期：2004-03-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Ophthalmic acid (OA; l-γ-glutamyl-l-2-aminobutyryl-glycine) is an analog of glutathione (GSH; l-γ-glutamyl-l-cysteinyl-glycine) in which the cysteine moiety is replaced by l-2-aminobutyrate. OA is a useful peptide for the pharmaceutical and/or food industries. Herein, we report a method for the production of OA using ...

journal_title：Applied and environmental microbiology

authors： Ito T,Tokoro M,Hori R,Hemmi H,Yoshimura T

更新日期：2018-03-19 00:00:00

abstract：:The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...

journal_title：Applied and environmental microbiology

authors： Sela S,Nestel D,Pinto R,Nemny-Lavy E,Bar-Joseph M

更新日期：2005-07-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used. A broad diversity of eukaryotic DNA sequences that...

journal_title：Applied and environmental microbiology

authors： Lydolph MC,Jacobsen J,Arctander P,Gilbert MT,Gilichinsky DA,Hansen AJ,Willerslev E,Lange L

更新日期：2005-02-01 00:00:00

abstract：:The initial formation and spatiotemporal development of microbial biofilm layers on surfaces of new and clean reverse osmosis (RO) membranes and feed-side spacers were monitored in situ using flow cells placed in parallel with the RO system of a full-scale water treatment plant. The feed water of the RO system had bee...

journal_title：Applied and environmental microbiology

authors： Bereschenko LA,Stams AJ,Euverink GJ,van Loosdrecht MC

更新日期：2010-04-01 00:00:00

abstract：:To investigate the microbiology and epidemiology of the 1,700 sporadic cases of listeriosis that occur annually in the United States, we developed a multilocus enzyme electrophoresis (MEE) typing system for Listeria monocytogenes. We studied 390 isolates by MEE. Eighty-two electrophoretic types (ETs) were defined. Two...

journal_title：Applied and environmental microbiology

authors： Bibb WF,Gellin BG,Weaver R,Schwartz B,Plikaytis BD,Reeves MW,Pinner RW,Broome CV

更新日期：1990-07-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carr...

journal_title：Applied and environmental microbiology

authors： Deleo PC,Baveye P

更新日期：1996-12-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:Growth and succinate versus lactate production from glucose by Anaerobiospirillum succiniciproducens was regulated by the level of available carbon dioxide and culture pH. At pH 7.2, the generation time was almost doubled and extensive amounts of lactate were formed in comparison with growth at pH 6.2. The succinate y...

journal_title：Applied and environmental microbiology

authors： Samuelov NS,Lamed R,Lowe S,Zeikus JG

更新日期：1991-10-01 00:00:00

abstract：:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Kohler HP,van der Maarel MJ,Kohler-Staub D

更新日期：1993-03-01 00:00:00

abstract：:The transposon Tn916 and self-mobilizing plasmid pAM beta 1 were conjugated from Enterococcus faecalis to the ruminal bacterium Streptococcus bovis JB1. Transconjugants were identified by resistance to tetracycline (Tn916) or erythromycin (pAM beta 1) and by Southern hybridization analyses. Transfer frequencies were 7...

journal_title：Applied and environmental microbiology

authors： Hespell RB,Whitehead TR

更新日期：1991-09-01 00:00:00

abstract：:The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Lundgren BV,Allard B,Rosswall T

更新日期：1988-07-01 00:00:00

abstract：:Diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the United States, was studied to determine its potential role as a vehicle of Salmonella enterica serotype Poona, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogen...

journal_title：Applied and environmental microbiology

authors： Gibbs DS,Anderson GL,Beuchat LR,Carta LK,Williams PL

更新日期：2005-05-01 00:00:00

abstract：:Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...

journal_title：Applied and environmental microbiology

authors： Huff WE,Doerr JA,Hamilton PB

更新日期：1979-01-01 00:00:00

abstract：:A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

journal_title：Applied and environmental microbiology

authors： McMullan G,Harrington F,Quinn JP

更新日期：1992-04-01 00:00:00