Abstract:
:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active forms of Ras have growth medium-dependent phenotypes. In nutrient-rich media (e.g., yeast extract and peptone), the phenotype of the transformants was indistinguishable from that of the wild type. However, during nutrient starvation, the transformants lose polarity, have distended hyphae, and fail to sporulate and produce appressoria. Since peptone caused the phenotype to revert, amino acids were tested singly and in combination to identify the responsible amino acid(s). We found that 1.6 mM proline in the medium reverses the constitutively active Ras phenotype.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Memmott SD,Ha YS,Dickman MBdoi
10.1128/aem.68.4.1647-1651.2002keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
1647-51issue
4eissn
0099-2240issn
1098-5336journal_volume
68pub_type
杂志文章abstract::A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup...
journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1990-04-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1989-02-01 00:00:00
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更新日期:2010-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1976-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:2014-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1995-04-01 00:00:00