Proline reverses the abnormal phenotypes of Colletotrichum trifolii associated with expression of endogenous constitutively active Ras.

Abstract:

:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active forms of Ras have growth medium-dependent phenotypes. In nutrient-rich media (e.g., yeast extract and peptone), the phenotype of the transformants was indistinguishable from that of the wild type. However, during nutrient starvation, the transformants lose polarity, have distended hyphae, and fail to sporulate and produce appressoria. Since peptone caused the phenotype to revert, amino acids were tested singly and in combination to identify the responsible amino acid(s). We found that 1.6 mM proline in the medium reverses the constitutively active Ras phenotype.

journal_name

Appl Environ Microbiol

authors

Memmott SD,Ha YS,Dickman MB

doi

10.1128/aem.68.4.1647-1651.2002

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

1647-51

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

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