Bacteria associated with false-positive most-probable-number coliform test results for shellfish and estuaries.

abstract：:To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

journal_title：Applied and environmental microbiology

authors： Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

更新日期：2020-01-21 00:00:00

abstract：:Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

journal_title：Applied and environmental microbiology

authors： Halpern M,Gancz H,Broza M,Kashi Y

更新日期：2003-07-01 00:00:00

abstract：:The survival of Azospirillum brasilense Cd and Sp-245 in the rhizosphere of wheat and tomato plants and in 23 types of plant-free sterilized soils obtained from a wide range of environments in Israel and Mexico was evaluated. Large numbers of A. brasilense cells were detected in all the rhizospheres tested, regardless...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Puente ME,Rodriguez-Mendoza MN,Toledo G,Holguin G,Ferrera-Cerrato R,Pedrin S

更新日期：1995-05-01 00:00:00

abstract：:The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect f...

journal_title：Applied and environmental microbiology

authors： Busta FF,Baillie E,Murrell WG

更新日期：1976-08-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00

abstract：:A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

journal_title：Applied and environmental microbiology

authors： Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

更新日期：1995-12-01 00:00:00

abstract：:Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

journal_title：Applied and environmental microbiology

authors： Mallison SM 3rd,Cannon RE

更新日期：1984-05-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...

journal_title：Applied and environmental microbiology

authors： Yasugi M,Okuzaki D,Kuwana R,Takamatsu H,Fujita M,Sarker MR,Miyake M

更新日期：2016-05-02 00:00:00

abstract：:Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all...

journal_title：Applied and environmental microbiology

authors： Haas JH,Moore LW,Ream W,Manulis S

更新日期：1995-08-01 00:00:00

abstract：:Phytophthora ramorum is a recently described pathogen causing oak mortality (sudden oak death) in forests in coastal areas of California and southern Oregon and dieback and leaf blight in a range of tree, shrub, and herbaceous species in the United States and Europe. Due to the threat posed by this organism, stringent...

journal_title：Applied and environmental microbiology

authors： Tomlinson JA,Boonham N,Hughes KJ,Griffin RL,Barker I

更新日期：2005-11-01 00:00:00

abstract：:This study investigated the effect of a 30-cm covering of finished compost (FC) on survival of Escherichia coli O157:H7 and Salmonella spp. in active static and windrow composting systems. Feedstocks inoculated with E. coli O157:H7 (7.41 log CFU/g) and Salmonella (6.46 log CFU/g) were placed in biosentry tubes (7.5-cm...

journal_title：Applied and environmental microbiology

authors： Patel JR,Yossa I,Macarisin D,Millner P

更新日期：2015-03-01 00:00:00

abstract：:Two new polyaromatic hydrocarbon-degrading marine bacteria have been isolated from burrow wall sediments of benthic macrofauna by using enrichments on phenanthrene. Strain LC8 (from a polychaete) and strain M4-6 (from a mollusc) are aerobic and gram negative and require sodium chloride (>1%) for growth. Both strains c...

journal_title：Applied and environmental microbiology

authors： Chung WK,King GM

更新日期：2001-12-01 00:00:00

abstract：:In Pseudomonas sp. strain M114, the outer membrane receptor for ferric pseudobactin M114 was shown to transport ferric pseudobactins B10 and A225, in addition to its own. The gene encoding this receptor, which was previously cloned on pCUP3, was localized by Tn5 mutagenesis to a region comprising >1.6 kb of M114 DNA. ...

journal_title：Applied and environmental microbiology

authors： Morris J,O'sullivan DJ,Koster M,Leong J,Weisbeek PJ,O'gara F

更新日期：1992-02-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are widely produced by Gram-positive bacteria, including many species of the Bacillus group. In the present study, one novel gene cluster coding lantibiotic cerecidins was unveiled in Bacillus cereus strain As 1.1846 ...

journal_title：Applied and environmental microbiology

authors： Wang J,Zhang L,Teng K,Sun S,Sun Z,Zhong J

更新日期：2014-04-01 00:00:00

abstract：:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron don...

journal_title：Applied and environmental microbiology

authors： Jiao Y,Kappler A,Croal LR,Newman DK

更新日期：2005-08-01 00:00:00

abstract：:Soil emission of gaseous N oxides during nitrification of ammonium represents loss of an available plant nutrient and has an important impact on the chemistry of the atmosphere. We used selective inhibitors and a glucose amendment in a factorial design to determine the relative contributions of autotrophic ammonium ox...

journal_title：Applied and environmental microbiology

authors： Tortoso AC,Hutchinson GL

更新日期：1990-06-01 00:00:00

abstract：:Viruses are the most abundant and diverse biological entities within soils, yet their ecological impact is largely unknown. Defining how soil viral communities change with perturbation or across environments will contribute to understanding the larger ecological significance of soil viruses. A new approach to examinin...

journal_title：Applied and environmental microbiology

authors： Srinivasiah S,Lovett J,Polson S,Bhavsar J,Ghosh D,Roy K,Fuhrmann JJ,Radosevich M,Wommack KE

更新日期：2013-09-01 00:00:00

abstract：:Organic acids derived from engineered microbes can replace fossil-derived chemicals in many applications. Fungal hosts are preferred for organic acid production because they tolerate lignocellulosic hydrolysates and low pH, allowing economic production and recovery of the free acid. However, cell death caused by cytos...

journal_title：Applied and environmental microbiology

authors： Zdraljevic S,Wagner D,Cheng K,Ruohonen L,Jäntti J,Penttilä M,Resnekov O,Pesce CG

更新日期：2013-12-01 00:00:00

abstract：:Nucleic acid-based analytical methods, ranging from species-targeted PCRs to metagenomics, have greatly expanded our understanding of microbiological diversity in natural samples. However, these methods provide only limited information on the activities and physiological states of microorganisms in samples. Even the m...

journal_title：Applied and environmental microbiology

authors： Cangelosi GA,Meschke JS

更新日期：2014-10-01 00:00:00

abstract：:The Sinorhizobium meliloti ORFeome project cloned 6,314 open reading frames (ORFs) into a modified Gateway entry vector system from which the ORFs could be transferred to destination vectors in vivo via bacterial conjugation. In this work, a reporter gene destination vector, pMK2030, was constructed and used to genera...

journal_title：Applied and environmental microbiology

authors： Humann JL,Schroeder BK,Mortimer MW,House BL,Yurgel SN,Maloney SC,Ward KL,Fallquist HM,Ziemkiewicz HT,Kahn ML

更新日期：2008-11-01 00:00:00

abstract：:An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

journal_title：Applied and environmental microbiology

authors： Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

更新日期：1989-09-01 00:00:00

abstract：:A method is described for generating and screening variants of the beta-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus sensitive to several environmental stresses, with potential application in the food industry. Chemical mutagenesis with hydroxylamine or methoxylamine was performed on the beta-galacto...

journal_title：Applied and environmental microbiology

authors： Yoast S,Adams RM,Mainzer SE,Moon K,Palombella AL,Schmidt BF

更新日期：1994-04-01 00:00:00

abstract：:Plasmid transfer rates for the conjugative plasmid R388::Tn1721 from Pseudomonas cepacia (donor) to Pseudomonas fluorescens (recipient) on agar media, in broth, and in microcosms containing sterile or nonsterile soil, in the presence or absence of germinating pea seeds, were determined. Donors, recipients, and transco...

journal_title：Applied and environmental microbiology

authors： Sudarshana P,Knudsen GR

更新日期：1995-08-01 00:00:00

abstract：:Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

journal_title：Applied and environmental microbiology

authors： Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

更新日期：2012-06-01 00:00:00

abstract：:Conjugal transfer of lactose-fermenting ability (Lac+), nisin resistance (Nisr), and phage resistance (Hsp+) was demonstrated in matings between Streptococcus lactis ME2 (donor) and Streptococcus cremoris M43a (recipient), a derivative of M12R. Transconjugants were detected by transfer of Lac+ and were found to exhibi...

journal_title：Applied and environmental microbiology

authors： Steenson LR,Klaenhammer TR

更新日期：1985-10-01 00:00:00

abstract：:Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

journal_title：Applied and environmental microbiology

authors： Fallon RD

更新日期：1992-09-01 00:00:00

abstract：:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

更新日期：2005-12-01 00:00:00

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00