Abstract:
:Nucleic acid-based analytical methods, ranging from species-targeted PCRs to metagenomics, have greatly expanded our understanding of microbiological diversity in natural samples. However, these methods provide only limited information on the activities and physiological states of microorganisms in samples. Even the most fundamental physiological state, viability, cannot be assessed cross-sectionally by standard DNA-targeted methods such as PCR. New PCR-based strategies, collectively called molecular viability analyses, have been developed that differentiate nucleic acids associated with viable cells from those associated with inactivated cells. In order to maximize the utility of these methods and to correctly interpret results, it is necessary to consider the physiological diversity of life and death in the microbial world. This article reviews molecular viability analysis in that context and discusses future opportunities for these strategies in genetic, metagenomic, and single-cell microbiology.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Cangelosi GA,Meschke JSdoi
10.1128/AEM.01763-14subject
Has Abstractpub_date
2014-10-01 00:00:00pages
5884-91issue
19eissn
0099-2240issn
1098-5336pii
AEM.01763-14journal_volume
80pub_type
杂志文章,评审abstract::The incorporation of tritiated thymidine by five microbial ecosystems and the distribution of tritium into DNA, RNA, and protein were determined. All microbial assemblages tested exhibited significant labeling of RNA and protein (i.e., nonspecific labeling), as determined by differential acid-base hydrolysis. Nonspeci...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.56.5.1245-1254.1990
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.56.10.3174-3178.1990
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更新日期:1986-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.5.1549-1554.1993
更新日期:1993-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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