Types and distribution of anaerobic bacteria in the large intestine of pigs.

Abstract:

:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x 10 organisms per g (dry weight) of material in the lumenal content, 14.0 x 10 in the surface layer, and 5.1 x 10 in the intestinal wall tissue. Both direct microscopic counts and viable culture counts were higher from the lumenal content and surface layer than from the intestinal tissue at all sites sampled in the intestine. Cultural counts averaged 56.2% of the direct microscopic counts in lumenal content and surface layer and 20.2% in intestinal tissue. Over 90% of the bacteria isolated were gram positive and consisted mainly of gram-positive cocci, lactobacilli, eubacteria, and clostridia. Of 192 isolates recovered, only 124 could be assigned to recognized species.

journal_name

Appl Environ Microbiol

authors

Russell EG

doi

10.1128/AEM.37.2.187-193.1979

keywords:

subject

Has Abstract

pub_date

1979-02-01 00:00:00

pages

187-93

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

37

pub_type

杂志文章
  • Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.

    abstract::α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.02581-12

    authors: Nikitkova AE,Haase EM,Scannapieco FA

    更新日期:2013-01-01 00:00:00

  • Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics.

    abstract::Contamination of food during processing is recognized as a main transmission route of Listeria monocytogenes To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01253-19

    authors: Roedel A,Dieckmann R,Brendebach H,Hammerl JA,Kleta S,Noll M,Al Dahouk S,Vincze S

    更新日期:2019-10-01 00:00:00

  • Amino acid profiles and presumptive nutritional assessment of single-cell protein from certain lactobacilli.

    abstract::The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.4.901-905.1977

    authors: Erdman MD,Bergen WG,Reddy CA

    更新日期:1977-04-01 00:00:00

  • Spoilage association of chicken leg muscle.

    abstract::The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.6.1244-1246.1977

    authors: McMeekin TA

    更新日期:1977-06-01 00:00:00

  • Efficacy of burning, tillage, and biocides in controlling bacteria released at field sites and effects on indigenous bacteria and fungi.

    abstract::Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.4.1207-1214.1992

    authors: Donegan K,Fieland V,Fowles N,Ganio L,Seidler R

    更新日期:1992-04-01 00:00:00

  • Deposition of manganese in a drinking water distribution system.

    abstract::The deposition of manganese in a water distribution system with manganese-related "dirty water" problems was studied over a 1-year period. Four monitoring laboratories with Robbins biofilm sampling devices fitted to the water mains were used to correlate the relationship among manganese deposition, the level of mangan...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.3.628-639.1990

    authors: Sly LI,Hodgkinson MC,Arunpairojana V

    更新日期:1990-03-01 00:00:00

  • The antifungal protein AFP from Aspergillus giganteus inhibits chitin synthesis in sensitive fungi.

    abstract::The antifungal protein AFP from Aspergillus giganteus is highly effective in restricting the growth of major human- and plant-pathogenic filamentous fungi. However, a fundamental prerequisite for the use of AFP as an antifungal drug is a complete understanding of its mode of action. In this study, we performed several...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02497-06

    authors: Hagen S,Marx F,Ram AF,Meyer V

    更新日期:2007-04-01 00:00:00

  • D-values of Bacillus pumilus spores on irradiated devices (inoculated product).

    abstract::The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.2.392-393.1978

    authors: Prince HN

    更新日期:1978-08-01 00:00:00

  • Population Structure and Morphotype Analysis of "Candidatus Accumulibacter" Using Fluorescence In Situ Hybridization-Staining-Flow Cytometry.

    abstract::"Candidatus Accumulibacter" is the dominant polyphosphate-accumulating organism (PAO) in denitrifying phosphorus removal (DPR) systems. In order to investigate the community structure and clade morphotypes of "Candidatus Accumulibacter" in DPR systems through flow cytometry (FCM), denitrifying phosphorus removal of al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02943-18

    authors: Li C,Zeng W,Li N,Guo Y,Peng Y

    更新日期:2019-04-18 00:00:00

  • Molecular monitoring of succession of bacterial communities in human neonates.

    abstract::The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.1.219-226.2002

    authors: Favier CF,Vaughan EE,De Vos WM,Akkermans AD

    更新日期:2002-01-01 00:00:00

  • Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application.

    abstract::Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02749-19

    authors: Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

    更新日期:2020-02-18 00:00:00

  • RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus.

    abstract::We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00315-08

    authors: Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

    更新日期:2008-10-01 00:00:00

  • Cometabolism of citrate and glucose by Enterococcus faecium FAIR-E 198 in the absence of cellular growth.

    abstract::Citrate metabolism by Enterococcus faecium FAIR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.319-326.2006

    authors: Vaningelgem F,Ghijsels V,Tsakalidou E,De Vuyst L

    更新日期:2006-01-01 00:00:00

  • Biotransformation of the antimelanoma agent betulinic acid by Bacillus megaterium ATCC 13368.

    abstract::Microbial transformation of the antimelanoma agent betulinic acid was studied. The main objective of this study was to utilize microorganisms as in vitro models to predict and prepare potential mammalian metabolites of this compound. Preparative-scale biotransformation with resting-cell suspensions of Bacillus megater...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3850-3855.2000

    authors: Chatterjee P,Kouzi SA,Pezzuto JM,Hamann MT

    更新日期:2000-09-01 00:00:00

  • Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli.

    abstract::Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00567-09

    authors: Miller EN,Jarboe LR,Yomano LP,York SW,Shanmugam KT,Ingram LO

    更新日期:2009-07-01 00:00:00

  • Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media.

    abstract::Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of ste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.2.206-208.1976

    authors: Rabie CJ,Lubben A,Steyn M

    更新日期:1976-08-01 00:00:00

  • Genes for the biosynthesis of the fungal polyketides hypothemycin from Hypomyces subiculosus and radicicol from Pochonia chlamydosporia.

    abstract::Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00478-08

    authors: Reeves CD,Hu Z,Reid R,Kealey JT

    更新日期:2008-08-01 00:00:00

  • Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment.

    abstract::During the summer of 1981, 3,887 sucrose-negative vibrios were isolated from seawater, sediment, plankton, and animal samples taken from 80 sites from Miami, Fla., to Portland, Maine. Of these, 4.2% were able to ferment lactose. The lactose-positive strains isolated from the various samples correlated positively with ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.985-998.1983

    authors: Oliver JD,Warner RA,Cleland DR

    更新日期:1983-03-01 00:00:00

  • Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex.

    abstract::The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00690-17

    authors: Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

    更新日期:2017-07-17 00:00:00

  • Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production.

    abstract::Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00249-12

    authors: Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

    更新日期:2012-07-01 00:00:00

  • Isolation of Thermus strains from hot composts (60 to 80 degrees C).

    abstract::High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.5.1723-1727.1996

    authors: Beffa T,Blanc M,Lyon PF,Vogt G,Marchiani M,Fischer JL,Aragno M

    更新日期:1996-05-01 00:00:00

  • Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons.

    abstract::Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4264-4267.1999

    authors: Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

    更新日期:1999-09-01 00:00:00

  • Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

    abstract::A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1642-1647.2005

    authors: Woodyer R,Simurdiak M,van der Donk WA,Zhao H

    更新日期:2005-03-01 00:00:00

  • Isolation and molecular characterization of chitinase-deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin.

    abstract::Proteolytic but chitinase-deficient microbial cultures were isolated from shrimp shell waste and characterized. The most efficient isolate was found to be a mixed culture consisting of two Bacillus licheniformis strains, which were first determined microscopically and physiologically. Molecular characterization was ca...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00938-06

    authors: Waldeck J,Daum G,Bisping B,Meinhardt F

    更新日期:2006-12-01 00:00:00

  • Persistence and leaching potential of microorganisms and mineral N in animal manure applied to intact soil columns.

    abstract::Pathogens may reach agricultural soils through application of animal manure and thereby pose a risk of contaminating crops as well as surface and groundwater. Treatment and handling of manure for improved nutrient and odor management may also influence the amount and fate of manure-borne pathogens in the soil. A study...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02506-12

    authors: Amin MG,Forslund A,Bui XT,Juhler RK,Petersen SO,Lægdsmand M

    更新日期:2013-01-01 00:00:00

  • Regulation of Lactobacillus casei sorbitol utilization genes requires DNA-binding transcriptional activator GutR and the conserved protein GutM.

    abstract::Sequence analysis of the five genes (gutRMCBA) downstream from the previously described sorbitol-6-phosphate dehydrogenase-encoding Lactobacillus casei gutF gene revealed that they constitute a sorbitol (glucitol) utilization operon. The gutRM genes encode putative regulators, while the gutCBA genes encode the EIIC, E...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00230-08

    authors: Alcántara C,Sarmiento-Rubiano LA,Monedero V,Deutscher J,Pérez-Martínez G,Yebra MJ

    更新日期:2008-09-01 00:00:00

  • Production of beta-galactosidase from Streptococcus thermophilus grown in whey.

    abstract::beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.2.185-188.1977

    authors: Rao MV,Dutta SM

    更新日期:1977-08-01 00:00:00

  • Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product.

    abstract::The beta-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase fami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00935-06

    authors: Dong J,Hashikawa S,Konishi T,Tamaru Y,Araki T

    更新日期:2006-09-01 00:00:00

  • Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

    abstract::The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01778-14

    authors: Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

    更新日期:2014-10-01 00:00:00

  • Effect of Colloidal Materials on Cellulase Production by Trichoderma reesei Rut-C30.

    abstract::The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, res...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.4.934-938.1985

    authors: Duff SJ,Cooper DG,Fuller OM

    更新日期:1985-04-01 00:00:00