Isolation and molecular characterization of chitinase-deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin.

abstract：:In the event of a biothreat agent release, hundreds of samples would need to be rapidly processed to characterize the extent of contamination and determine the efficacy of remediation activities. Current biological agent identification and viability determination methods are both labor- and time-intensive such that tu...

journal_title：Applied and environmental microbiology

authors： Létant SE,Murphy GA,Alfaro TM,Avila JR,Kane SR,Raber E,Bunt TM,Shah SR

更新日期：2011-09-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:Certain strains of Enterococcus faecium and Enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. To determine whether there are structural and functional genomic features that are distinct between nonclinical (NC) and clinical (CL) ...

journal_title：Applied and environmental microbiology

authors： Kim EB,Marco ML

更新日期：2014-01-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particu...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Oranje JP,Hijnen WA

更新日期：1982-11-01 00:00:00

abstract：:We characterized the intracellular symbiotic microbiota of the bamboo pseudococcid Antonina crawii by performing a molecular phylogenetic analysis in combination with in situ hybridization. Almost the entire length of the bacterial 16S rRNA gene was amplified and cloned from A. crawii whole DNA. Restriction fragment l...

journal_title：Applied and environmental microbiology

authors： Fukatsu T,Nikoh N

更新日期：2000-02-01 00:00:00

abstract：:Bacterial growth in biofilms is the major cause of recalcitrant biofouling in industrial processes and of persistent infections in clinical settings. The use of bacteriophage treatment to lyse bacteria in biofilms has attracted growing interest. In particular, many natural or engineered phages produce depolymerases to...

journal_title：Applied and environmental microbiology

authors： Pei R,Lamas-Samanamud GR

更新日期：2014-09-01 00:00:00

abstract：:Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

journal_title：Applied and environmental microbiology

authors： Macalady JL,Mack EE,Nelson DC,Scow KM

更新日期：2000-04-01 00:00:00

abstract：:The soil isolate Cellvibrio mixtus UQM2294 degraded a variety of polysaccharides including microcrystalline cellulose. Among 6,000 cosmid clones carrying C. mixtus DNA, constructed in Escherichia coli with pHC79, 50 expressed the ability to degrade one or more of the following substrates: carboxymethyl cellulose, chit...

journal_title：Applied and environmental microbiology

authors： Wynne EC,Pemberton JM

更新日期：1986-12-01 00:00:00

abstract：:Sequence analysis of the five genes (gutRMCBA) downstream from the previously described sorbitol-6-phosphate dehydrogenase-encoding Lactobacillus casei gutF gene revealed that they constitute a sorbitol (glucitol) utilization operon. The gutRM genes encode putative regulators, while the gutCBA genes encode the EIIC, E...

journal_title：Applied and environmental microbiology

authors： Alcántara C,Sarmiento-Rubiano LA,Monedero V,Deutscher J,Pérez-Martínez G,Yebra MJ

更新日期：2008-09-01 00:00:00

abstract：:Five autoagglutinating Aeromonas hydrophila isolates recovered from eels and humans were assigned to serogroups O:14 and O:81 of the Sakazaki and Shimada (National Institutes of Health) scheme. They had the following properties in common: positive precipitation after boiling, moderate surface hydrophobicity (salt-aggr...

journal_title：Applied and environmental microbiology

authors： Esteve C,Alcaide E,Canals R,Merino S,Blasco D,Figueras MJ,Tomás JM

更新日期：2004-10-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to approximately 60 mM glutathione when this compound was added to the medium. S...

journal_title：Applied and environmental microbiology

authors： Li Y,Hugenholtz J,Abee T,Molenaar D

更新日期：2003-10-01 00:00:00

abstract：:The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses, which are common in fresh water. This gene functions in vivo and also when cloned in Escherichia coli. Photodamaged viru...

journal_title：Applied and environmental microbiology

authors： Furuta M,Schrader JO,Schrader HS,Kokjohn TA,Nyaga S,McCullough AK,Lloyd RS,Burbank DE,Landstein D,Lane L,Van Etten JL

更新日期：1997-04-01 00:00:00

abstract：:The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

journal_title：Applied and environmental microbiology

authors： Gill AO,Holley RA

更新日期：2004-10-01 00:00:00

abstract：:Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result...

journal_title：Applied and environmental microbiology

authors： Repaske R,Mayer R

更新日期：1976-10-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical...

journal_title：Applied and environmental microbiology

authors： Perlińska-Lenart U,Orlowski J,Laudy AE,Zdebska E,Palamarczyk G,Kruszewska JS

更新日期：2006-12-01 00:00:00

abstract：:Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS g...

journal_title：Applied and environmental microbiology

authors： Tomás-Gallardo L,Santero E,Floriano B

更新日期：2012-08-01 00:00:00

abstract：:A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

journal_title：Applied and environmental microbiology

authors： Kennedy MJ,Lawless JG

更新日期：1985-01-01 00:00:00

abstract：:After propagation of Rhizopus javanicus in defined media containing glucose, urea, and mineral salts in deionized distilled water, the ability of the nonliving biomass to sequester cupric ion was assayed. Growth, uptake capacity (saturation uptake at >1 mM Cu concentration in solution), and biosorptive yield (biomass ...

journal_title：Applied and environmental microbiology

authors： Treen-Sears ME,Martin SM,Volesky B

更新日期：1984-07-01 00:00:00

abstract：:The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the leading vector-borne illness in the United States. Many of the genetic factors affecting spirochete morphology and physiology are unknown due to the limited genetic tools available and the large number of open reading frames with unknown f...

journal_title：Applied and environmental microbiology

authors： Stewart PE,Hoff J,Fischer E,Krum JG,Rosa PA

更新日期：2004-10-01 00:00:00

abstract：:The initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. Characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. Usi...

journal_title：Applied and environmental microbiology

authors： Diaz PI,Chalmers NI,Rickard AH,Kong C,Milburn CL,Palmer RJ Jr,Kolenbrander PE

更新日期：2006-04-01 00:00:00

abstract：:Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

journal_title：Applied and environmental microbiology

authors： Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

更新日期：1989-08-01 00:00:00

abstract：:Ciprofloxacin (CIP), a fluoroquinolone antibacterial drug, is widely used in the treatment of serious infections in humans. Its degradation by basidiomycetous fungi was studied by monitoring 14CO2 production from [14C]CIP in liquid cultures. Sixteen species inhabiting wood, soil, humus, or animal dung produced up to 3...

journal_title：Applied and environmental microbiology

authors： Wetzstein HG,Stadler M,Tichy HV,Dalhoff A,Karl W

更新日期：1999-04-01 00:00:00

abstract：:By using an autoradiographic technique, one can follow, during ripening of a cheese, the distribution, size, and metabolic activity of microcolonies. Fragments of cheese were labeled with [H]leucine, fixed, and mounted in epoxy resin. After exposure and development, sections were examined by optical microscopy. In Cam...

journal_title：Applied and environmental microbiology

authors： Reyrolle J,Letellier F

更新日期：1979-12-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the United States. Though multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus strains have been reported, resistance patterns in vibrios ...

journal_title：Applied and environmental microbiology

authors： Elmahdi S,Parveen S,Ossai S,DaSilva LV,Jahncke M,Bowers J,Jacobs J

更新日期：2018-01-17 00:00:00

abstract：:We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

journal_title：Applied and environmental microbiology

authors： Huertas MJ,Duque E,Marqués S,Ramos JL

更新日期：1998-01-01 00:00:00

abstract：:Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. ...

journal_title：Applied and environmental microbiology

authors： King AD Jr,Hocking AD,Pitt JI

更新日期：1979-05-01 00:00:00

abstract：:The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model...

journal_title：Applied and environmental microbiology

authors： Fujikawa H,Itoh T

更新日期：1996-10-01 00:00:00