Pathogenic Aeromonas hydrophila serogroup O:14 and O:81 strains with an S layer.

Abstract:

:Five autoagglutinating Aeromonas hydrophila isolates recovered from eels and humans were assigned to serogroups O:14 and O:81 of the Sakazaki and Shimada (National Institutes of Health) scheme. They had the following properties in common: positive precipitation after boiling, moderate surface hydrophobicity (salt-aggregation-test value around 1.2), pathogenicity for fish and mice (50% lethal dose, 10(4.61) to 10(7.11)), lipopolysaccharides that contained O-polysaccharide chains of homogeneous chain length, and an external S layer peripheral to the cell wall observed by electron microscopy. A strong cross-reactivity was detected by immunoblotting between the homogeneous O-polysaccharide fraction of O:14 and O:81 strains but not between them and the lipopolysaccharide of A. hydrophila TF7 (O:11 reference strain). Outer membrane fractions of these strains contained a predominant 53- to 54-kDa protein which was glycine extractable under low-pH (pH 2.8) conditions and was identified as the surface array protein. The S-layer proteins of the O:14 and O:81 A. hydrophila strains seemed to be primarily different from those previously purified from strains A. hydrophila TF7 and Aeromonas salmonicida A450 on the basis of colony hybridizations with both the structural genes vapA and ahsA. This is the first report of the presence of an S layer in mesophilic Aeromonas strains not belonging to serogroup O:11.

journal_name

Appl Environ Microbiol

authors

Esteve C,Alcaide E,Canals R,Merino S,Blasco D,Figueras MJ,Tomás JM

doi

10.1128/AEM.70.10.5898-5904.2004

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

5898-904

issue

10

eissn

0099-2240

issn

1098-5336

pii

70/10/5898

journal_volume

70

pub_type

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