Bacterial biodegradation of extractives and patterns of bordered pit membrane attack in pine wood.

abstract：:Reservoir 29 and Lake B are extremely acid lakes (epilimnion pHs of 2.7 and 3.2, respectively), because they receive acidic discharges from coal refuse piles. They differ in that the pH of profundal sediments in Reservoir 29 increased from 2.7 to 3.8 during the period of thermal stratification, whereas permanently ano...

journal_title：Applied and environmental microbiology

authors： Gyure RA,Konopka A,Brooks A,Doemel W

更新日期：1987-09-01 00:00:00

abstract：:Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with dif...

journal_title：Applied and environmental microbiology

authors： Muniz Mde M,Morais E Silva Tavares P,Meyer W,Nosanchuk JD,Zancope-Oliveira RM

更新日期：2010-07-01 00:00:00

abstract：:Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activi...

journal_title：Applied and environmental microbiology

authors： Saha BC,Zeikus JG

更新日期：1990-09-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:An accurate most-probable-number enumeration method was developed for counting the number of Beggiatoa trichomes from various freshwater sediments. The medium consisted of extracted hay, diluted soil extract, 0.05% acetate, and 15 to 35 U of catalase per ml. The same enrichment medium, but without the acetate, was the...

journal_title：Applied and environmental microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-11-01 00:00:00

abstract：:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, req...

journal_title：Applied and environmental microbiology

authors： Yung PT,Ponce A

更新日期：2008-12-01 00:00:00

abstract：:Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a deri...

journal_title：Applied and environmental microbiology

authors： Villacieros M,Whelan C,Mackova M,Molgaard J,Sánchez-Contreras M,Lloret J,Aguirre de Cárcer D,Oruezábal RI,Bolaños L,Macek T,Karlson U,Dowling DN,Martín M,Rivilla R

更新日期：2005-05-01 00:00:00

abstract：:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

journal_title：Applied and environmental microbiology

authors： Cleveland TE,Lax AR,Lee LS,Bhatnagar D

更新日期：1987-07-01 00:00:00

abstract：:Borrelia spirochetes in bird-feeding ticks were studied in the Czech Republic. During the postbreeding period (July to September 2005), 1,080 passerine birds infested by 2,240 Ixodes ricinus subadult ticks were examined. Borrelia garinii was detected in 22.2% of the ticks, Borrelia valaisiana was detected in 12.8% of ...

journal_title：Applied and environmental microbiology

authors： Dubska L,Literak I,Kocianova E,Taragelova V,Sychra O

更新日期：2009-02-01 00:00:00

abstract：:We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

更新日期：2005-04-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...

journal_title：Applied and environmental microbiology

authors： Chu YM,Jeon JJ,Yea SJ,Kim YH,Yun SH,Lee YW,Kim KH

更新日期：2002-05-01 00:00:00

abstract：:Cadmium is an important heavy metal pollutant. For this study, we investigated the effects of cadmium exposure on the oxidative stress responses of Xanthomonas campestris, a soil and plant pathogenic bacterium. The exposure of X. campestris to low concentrations of cadmium induces cross-protection against subsequent k...

journal_title：Applied and environmental microbiology

authors： Banjerdkij P,Vattanaviboon P,Mongkolsuk S

更新日期：2005-04-01 00:00:00

abstract：:The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced...

journal_title：Applied and environmental microbiology

authors： Gouesbet G,Jan G,Boyaval P

更新日期：2002-03-01 00:00:00

abstract：:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose ...

journal_title：Applied and environmental microbiology

authors： Feng X,Mouttaki H,Lin L,Huang R,Wu B,Hemme CL,He Z,Zhang B,Hicks LM,Xu J,Zhou J,Tang YJ

更新日期：2009-08-01 00:00:00

abstract：:The existence of anaerobic biodegradation of lignin was examined in mixed microflora. Egyptian soil samples, in which rapid mineralization of organic matter takes place in the presence of an important anaerobic microflora, were used to obtain the anaerobic enrichment cultures for this study. Specifically, 14CO2 or [14...

journal_title：Applied and environmental microbiology

authors： Odier E,Monties B

更新日期：1983-09-01 00:00:00

abstract：:The effect of autoproteolysis of Lactococcus lactis lactocepin III on its specificity towards beta-casein was investigated. beta-Casein degradation was performed by using either an autolysin-defective derivative of L. lactis MG1363 carrying the proteinase genes of L. lactis SK11, which was unable to transport oligopep...

journal_title：Applied and environmental microbiology

authors： Flambard B,Juillard V

更新日期：2000-12-01 00:00:00

abstract：:A sublethal dose of ethanol (5%, vol/vol), acid (HCl, pH 4.5 to 5.0), H2O2 (500 ppm), or NaCl (7%, wt/vol) was added to a Listeria monocytogenes culture at the exponential phase, and the cells were allowed to grow for 1 h. Exponential-phase cells also were heat shocked at 45 degrees C for 1 h. The stress-adapted cells...

journal_title：Applied and environmental microbiology

authors： Lou Y,Yousef AE

更新日期：1997-04-01 00:00:00

abstract：:Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen ...

journal_title：Applied and environmental microbiology

authors： Gibbons RJ,Dankers I

更新日期：1981-04-01 00:00:00

abstract：:Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remi...

journal_title：Applied and environmental microbiology

authors： Beaman TC,Gerhardt P

更新日期：1986-12-01 00:00:00

abstract：:Trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of Vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from Vibrio alginolyticus and other bacteria. ...

journal_title：Applied and environmental microbiology

authors： Kourany M

更新日期：1983-01-01 00:00:00

abstract：:A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

journal_title：Applied and environmental microbiology

authors： Gorski TW,Ritzert RW

更新日期：1977-08-01 00:00:00

abstract：:In Corynebacterium glutamicum, the ArgR protein, a transcriptional repressor, affects the expression level of the argB gene through binding to its promoter region. The argB promoter region (positions -77 to -25) has been found by in vitro electrophoretic mobility shift assay (EMSA) results and in silico analysis to be...

journal_title：Applied and environmental microbiology

authors： Lee SY,Park JM,Lee JH,Chang ST,Park JS,Kim YH,Min J

更新日期：2011-02-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical...

journal_title：Applied and environmental microbiology

authors： Perlińska-Lenart U,Orlowski J,Laudy AE,Zdebska E,Palamarczyk G,Kruszewska JS

更新日期：2006-12-01 00:00:00

abstract：:Of 70 pure microbial cultures isolated from aquatic habitats, soil, and air according to the ability to lyse live blue-green algae, 62 were fungi representing the genera Acremonium, Emericellopsis, and Verticillium. Algal-lysing fungi were isolated from all habitat types sampled. The remaining isolates comprised four ...

journal_title：Applied and environmental microbiology

authors： Redhead K,Wright SJ

更新日期：1978-05-01 00:00:00

abstract：:The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflamma...

journal_title：Applied and environmental microbiology

authors： Wang Z,Wang J,Cheng Y,Liu X,Huang Y

更新日期：2011-11-01 00:00:00

abstract：UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

journal_title：Applied and environmental microbiology

authors： Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

更新日期：2016-05-16 00:00:00

abstract：:Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number o...

journal_title：Applied and environmental microbiology

authors： Sanders SW,Maxcy RB

更新日期：1979-09-01 00:00:00

abstract：:Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, wh...

journal_title：Applied and environmental microbiology

authors： Coletta-Filho HD,Takita MA,de Souza AA,Aguilar-Vildoso CI,Machado MA

更新日期：2001-09-01 00:00:00

abstract：:The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...

journal_title：Applied and environmental microbiology

authors： Shu C,Liu D,Zhou Z,Cai J,Peng Q,Gao J,Song F,Zhang J

更新日期：2013-11-01 00:00:00

abstract：:Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

journal_title：Applied and environmental microbiology

authors： Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

更新日期：1989-08-01 00:00:00