Fast sterility assessment by germinable-endospore biodosimetry.

Abstract:

:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, requiring more than 2 days before results become available, are employed to verify endospore inactivation. We describe a rapid, microscopy-based endospore viability assay (microEVA) capable of enumerating germinable endospores in less than 15 min. MicroEVA employs time-gated luminescence microscopy to enumerate single germinable endospores via terbium-dipicolinate (Tb-DPA) luminescence, which is triggered under UV excitation as 10(8) DPA molecules are released during germination on agarose containing Tb(3+) and a germinant (e.g., L-alanine). Inactivation of endospore populations to sterility was monitored with microEVA as a function of thermal and UV dosage. A comparison of culturing results yielded nearly identical decimal reduction values, thus validating microEVA as a rapid biodosimetry method for monitoring sterilization processes. The simple Tb-DPA chemical test for germinability is envisioned to enable fully automated instrumentation for in-line monitoring of hygiene in industrial production processes.

journal_name

Appl Environ Microbiol

authors

Yung PT,Ponce A

doi

10.1128/AEM.01437-08

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

7669-74

issue

24

eissn

0099-2240

issn

1098-5336

pii

AEM.01437-08

journal_volume

74

pub_type

杂志文章
  • Production of Lactase by Candida pseudotropicalis Grown in Whey.

    abstract::Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.6.1201-1205.1979

    authors: de Bales SA,Castillo FJ

    更新日期:1979-06-01 00:00:00

  • Effect of benzoic Acid on growth yield of yeasts differing in their resistance to preservatives.

    abstract::Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.8.2091-2095.1988

    authors: Warth AD

    更新日期:1988-08-01 00:00:00

  • Genotypic and antibiotic susceptibility characteristics of a Campylobacter coli population isolated from dairy farmland in the United Kingdom.

    abstract::Campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by Campylobacter coli. Most studies have concentrated on Campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.2.822-830.2004

    authors: Leatherbarrow AJ,Hart CA,Kemp R,Williams NJ,Ridley A,Sharma M,Diggle PJ,Wright EJ,Sutherst J,French NP

    更新日期:2004-02-01 00:00:00

  • Comparison of cytotoxicity and thin-layer chromatography methods for detection of mycotoxins.

    abstract::Thirty-three standard mycotoxins were assayed by thin-layer chromatography and by cytotoxicity in HEp-2 and Chang cells. Various levels of detection were found. The cytotoxicity test was significantly more sensitive than thin-layer chromatography for the trichothecenes and should be useful for screening extracts from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.4.948-950.1983

    authors: Robb J,Norval M

    更新日期:1983-10-01 00:00:00

  • Evaluation of sample recovery efficiency for bacteriophage P22 on fomites.

    abstract::Fomites are known to play a role in the transmission of pathogens. Quantitative analysis of the parameters that affect sample recovery efficiency (SRE) at the limit of detection of viruses on fomites will aid in improving quantitative microbial risk assessment (QMRA) and infection control. The variability in SRE as a ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01370-12

    authors: Herzog AB,Pandey AK,Reyes-Gastelum D,Gerba CP,Rose JB,Hashsham SA

    更新日期:2012-11-01 00:00:00

  • Free-living Rhizobium strain able to grow on n(2) as the sole nitrogen source.

    abstract::[This corrects the article on p. 711 in vol. 45.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:

    authors:

    更新日期:1983-11-01 00:00:00

  • Assessing Transmission of Antimicrobial-Resistant Escherichia coli in Wild Giraffe Contact Networks.

    abstract::There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02136-18

    authors: Miller EA,Johnson TJ,Omondi G,Atwill ER,Isbell LA,McCowan B,VanderWaal K

    更新日期:2018-12-13 00:00:00

  • Adsorption of Agrobacterium tumefaciens to Susceptible Potato Tissues: a Physisorption Process.

    abstract::Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.5.1130-1132.1986

    authors: Kluepfel DA,Pueppke SG

    更新日期:1986-05-01 00:00:00

  • Characterization of DC1, a broad-host-range Bcep22-like podovirus.

    abstract::Bcep22-like phages are a recently described group of podoviruses that infect strains of Burkholderia cenocepacia. We have isolated and characterized a novel member of this group named DC1. This podovirus shows many genomic similarities to BcepIL02 and Bcep22, but it infects strains belonging to multiple Burkholderia c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07097-11

    authors: Lynch KH,Stothard P,Dennis JJ

    更新日期:2012-02-01 00:00:00

  • Persistence of the 2009 pandemic influenza A (H1N1) virus on N95 respirators.

    abstract::In the United States, the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths from April 2009 to April 2010. On 24 April 2009, the CDC posted interim guidance on infection control measures in health care settings explicitly for pH...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03850-12

    authors: Coulliette AD,Perry KA,Edwards JR,Noble-Wang JA

    更新日期:2013-04-01 00:00:00

  • Extensive reduction of cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with a D-amino acid mixture.

    abstract::Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02911-12

    authors: Sanchez Z,Tani A,Kimbara K

    更新日期:2013-02-01 00:00:00

  • Fitness of Salmonella enterica serovar Thompson in the cilantro phyllosphere.

    abstract::The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.7.3614-3621.2002

    authors: Brandl MT,Mandrell RE

    更新日期:2002-07-01 00:00:00

  • Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults.

    abstract:UNLABELLED:Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in a healthy population. We conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of Bifidobacterium bifidum strain Bb on the IME of adult hea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1128/AEM.01753-16

    authors: Gargari G,Taverniti V,Balzaretti S,Ferrario C,Gardana C,Simonetti P,Guglielmetti S

    更新日期:2016-09-16 00:00:00

  • New method for isolating barophiles from intestinal contents of deep-sea fishes retrieved from the abyssal zone.

    abstract::We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.11.4210-4212.1994

    authors: Nakayama A,Yano Y,Yoshida K

    更新日期:1994-11-01 00:00:00

  • Taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type I propagation using Lactobacillus sanfranciscensis starters.

    abstract::The structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type I propagation by using singly nine strains of Lactobacillus sanfranciscensis. Under back-slopping propagation with wheat flour type 0 F114, cell numbers of presumptive lactic acid bacteria varie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01524-08

    authors: Siragusa S,Di Cagno R,Ercolini D,Minervini F,Gobbetti M,De Angelis M

    更新日期:2009-02-01 00:00:00

  • Imidacloprid Decreases Honey Bee Survival Rates but Does Not Affect the Gut Microbiome.

    abstract::Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00545-18

    authors: Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

    更新日期:2018-06-18 00:00:00

  • Dynamics of the methanogenic archaeal community during plant residue decomposition in an anoxic rice field soil.

    abstract::Incorporation of plant residues strongly enhances the methane production and emission from flooded rice fields. Temperature and residue type are important factors that regulate residue decomposition and CH(4) production. However, the response of the methanogenic archaeal community to these factors in rice field soil i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00070-08

    authors: Peng J,Lü Z,Rui J,Lu Y

    更新日期:2008-05-01 00:00:00

  • A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom (Flammulina velutipes).

    abstract::Most of the edible mushrooms cannot be cultivated or have low yield under industrial conditions, partially due to the lack of knowledge on how basidioma (fruiting body) development is regulated. From winter mushroom (Flammulina velutipes), one of the most popular industrially cultivated mushrooms, a transcription fact...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01735-19

    authors: Wu T,Hu C,Xie B,Zhang L,Yan S,Wang W,Tao Y,Li S

    更新日期:2019-11-27 00:00:00

  • Functional Analysis of a Novel β-(1,3)-Glucanase from Corallococcus sp. Strain EGB Containing a Fascin-Like Module.

    abstract::A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01016-17

    authors: Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

    更新日期:2017-08-01 00:00:00

  • Heat inactivation of in vivo- and in vitro-grown mycobacteria in meat products.

    abstract::Heat inactivation of mycobacteria from lesions and from culture was compared in meat products. In vivo-grown organisms were more easily heat inactivated than were in vitro-grown organisms. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.6.1484-1485.1981

    authors: Merkal RS,Lyle PS,Whipple DL

    更新日期:1981-06-01 00:00:00

  • Metabolic and Genotypic Fingerprinting of Fluorescent Pseudomonads Associated with the Douglas Fir-Laccaria bicolor Mycorrhizosphere.

    abstract::A collection of 300 isolates of fluorescent pseudomonads was established from Douglas fir-Laccaria bicolor mycorrhizas and mycorrhizosphere and from adjacent bulk soil. These isolates were first phenotypically characterized with the Biolog method. Taxonomic identification assigned 90% of the isolates to the different ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.5.1852-1860.1997

    authors: Frey P,Frey-Klett P,Garbaye J,Berge O,Heulin T

    更新日期:1997-05-01 00:00:00

  • Management of Multiple Nitrogen Sources during Wine Fermentation by Saccharomyces cerevisiae.

    abstract::During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02617-16

    authors: Crépin L,Truong NM,Bloem A,Sanchez I,Dequin S,Camarasa C

    更新日期:2017-02-15 00:00:00

  • Tolerance of Listeria monocytogenes to Quaternary Ammonium Sanitizers Is Mediated by a Novel Efflux Pump Encoded by emrE.

    abstract::A novel genomic island (LGI1) was discovered in Listeria monocytogenes isolates responsible for the deadliest listeriosis outbreak in Canada, in 2008. To investigate the functional role of LGI1, the outbreak strain 08-5578 was exposed to food chain-relevant stresses, and the expression of 16 LGI1 genes was measured. L...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03741-15

    authors: Kovacevic J,Ziegler J,Wałecka-Zacharska E,Reimer A,Kitts DD,Gilmour MW

    更新日期:2015-11-20 00:00:00

  • ESKAPE Bacteria and Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolated from Wastewater and Process Water from German Poultry Slaughterhouses.

    abstract::The wastewater of livestock slaughterhouses is considered a source of antimicrobial-resistant bacteria with clinical relevance and may thus be important for their dissemination into the environment. To get an overview of their occurrence and characteristics, we investigated process water (n = 50) from delivery and unc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02748-19

    authors: Savin M,Bierbaum G,Hammerl JA,Heinemann C,Parcina M,Sib E,Voigt A,Kreyenschmidt J

    更新日期:2020-04-01 00:00:00

  • Cell-Free Solubilization of Coal by Polyporus versicolor.

    abstract::Solubilization of coal was demonstrated with filtrates (0.45-mum-pore-size filters) obtained from the broth in which Polyporus versicolor had grown. The rate and extent of solubilization were dependent on the age of the fungal cultures, the particle size of the coal, the pH of the filtrates, and the presence of protei...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2840-2843.1987

    authors: Cohen MS,Bowers WC,Aronson H,Gray ET

    更新日期:1987-12-01 00:00:00

  • Generation of polyclonal antibodies of predetermined specificity against pediocin PA-1.

    abstract::Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-gener...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4536-4545.1998

    authors: Martínez JM,Martínez MI,Suárez AM,Herranz C,Casaus P,Cintas LM,Rodríguez JM,Hernández PE

    更新日期:1998-11-01 00:00:00

  • Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones.

    abstract::More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5248-5254.2003

    authors: Hahn MW

    更新日期:2003-09-01 00:00:00

  • Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici.

    abstract::Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2534-2538.1987

    authors: Gonzalez CF,Kunka BS

    更新日期:1987-10-01 00:00:00

  • Copper Reduction and Contact Killing of Bacteria by Iron Surfaces.

    abstract::The well-established killing of bacteria by copper surfaces, also called contact killing, is currently believed to be a combined effect of bacterial contact with the copper surface and the dissolution of copper, resulting in lethal bacterial damage. Iron can similarly be released in ionic form from iron surfaces and w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01725-15

    authors: Mathews S,Kumar R,Solioz M

    更新日期:2015-09-01 00:00:00

  • Competitiveness of Rhizobium trifolii Strains Associated with Red Clover (Trifolium pratense L.) in Mississippi Soils.

    abstract::Five strains of Rhizobium trifolii were evaluated in competition with indigenous populations in nodulating red clover (Trifolium pratense L.) cv. Kenland in two different soils in Mississippi. Double antibiotic resistance acquisition was used to measure the proportion of nodules occupied by the introduced mutant strai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.5.1096-1101.1982

    authors: Materon LA,Hagedorn C

    更新日期:1982-11-01 00:00:00