Abstract:
:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, requiring more than 2 days before results become available, are employed to verify endospore inactivation. We describe a rapid, microscopy-based endospore viability assay (microEVA) capable of enumerating germinable endospores in less than 15 min. MicroEVA employs time-gated luminescence microscopy to enumerate single germinable endospores via terbium-dipicolinate (Tb-DPA) luminescence, which is triggered under UV excitation as 10(8) DPA molecules are released during germination on agarose containing Tb(3+) and a germinant (e.g., L-alanine). Inactivation of endospore populations to sterility was monitored with microEVA as a function of thermal and UV dosage. A comparison of culturing results yielded nearly identical decimal reduction values, thus validating microEVA as a rapid biodosimetry method for monitoring sterilization processes. The simple Tb-DPA chemical test for germinability is envisioned to enable fully automated instrumentation for in-line monitoring of hygiene in industrial production processes.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Yung PT,Ponce Adoi
10.1128/AEM.01437-08subject
Has Abstractpub_date
2008-12-01 00:00:00pages
7669-74issue
24eissn
0099-2240issn
1098-5336pii
AEM.01437-08journal_volume
74pub_type
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doi:10.1128/AEM.60.11.4210-4212.1994
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pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.64.11.4536-4545.1998
更新日期:1998-11-01 00:00:00
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更新日期:1987-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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