Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici.

abstract：:Disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) gave Aspergillus parasiticus transformants that accumulated versicolorin A. This gene is predicted to encode the Baeyer-Villiger oxidase necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin. ...

journal_title：Applied and environmental microbiology

authors： Ehrlich KC,Montalbano B,Boué SM,Bhatnagar D

更新日期：2005-12-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:The effects of Cd2+, Cu2+, Mg2+, and Zn2+ on the decomposition of citric acid by a Klebsiella sp. were studied by monitoring the degradation of [14C]citrate. The carbon concentration used was 10 micrograms of C liter-1, and the media were designed to provide at least 95% of the citrate complexed to the metal studied. ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Rosswall T

更新日期：1989-06-01 00:00:00

abstract：:Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic ...

journal_title：Applied and environmental microbiology

authors： Turner JT,Lampel JS,Stearman RS,Sundin GW,Gunyuzlu P,Anderson JJ

更新日期：1991-12-01 00:00:00

abstract：:In 2004, more than 1,230 cases of gastroenteritis due to pandemic O3:K6 strains of Vibrio parahaemolyticus were reported in southern Sinaloa, a state in Northwestern Mexico. Recurrent sporadic cases arose from 2004 to 2010, spreading from the south to the north. In the present study, Vibrio parahaemolyticus was detect...

journal_title：Applied and environmental microbiology

authors： Velazquez-Roman J,León-Sicairos N,Flores-Villaseñor H,Villafaña-Rauda S,Canizalez-Roman A

更新日期：2012-03-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a dom...

journal_title：Applied and environmental microbiology

authors： Jeon SD,Lee JE,Kim SJ,Park SH,Choi GW,Han SO

更新日期：2013-10-01 00:00:00

abstract：:Accurate predictions of carbon and energy cycling rates in the environment depend on sampling frequencies and on the spatial variability associated with biological activities. We examined the variability associated with anaerobic biodegradation rates at two sites in an alluvial sand aquifer polluted by municipal landf...

journal_title：Applied and environmental microbiology

authors： Adrian NR,Robinson JA,Suflita JM

更新日期：1994-10-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with ga...

journal_title：Applied and environmental microbiology

authors： Kansiz M,Billman-Jacobe H,McNaughton D

更新日期：2000-08-01 00:00:00

abstract：:The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Lundgren BV,Allard B,Rosswall T

更新日期：1988-07-01 00:00:00

abstract：:A dissimilatory Fe(III)- and Mn(IV)-reducing bacterium was isolated from bottom sediments of the Great Bay estuary, New Hampshire. The isolate was a facultatively anaerobic gram-negative rod which did not appear to fit into any previously described genus. It was temporarily designated strain BrY. BrY grew anaerobicall...

journal_title：Applied and environmental microbiology

authors： Caccavo F,Blakemore RP,Lovley DR

更新日期：1992-10-01 00:00:00

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for ...

journal_title：Applied and environmental microbiology

authors： Rayamajhi N,Cha SB,Kang ML,Lee SI,Lee HS,Yoo HS

更新日期：2009-09-01 00:00:00

abstract：:DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in solution. In addition, PNA molecular beacon hybridizations were found to be useful for the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA concentrations used in this experiment (...

journal_title：Applied and environmental microbiology

authors： Xi C,Balberg M,Boppart SA,Raskin L

更新日期：2003-09-01 00:00:00

abstract：:The biogeography of the purple nonsulfur bacterium Rhodopseudomonas palustris on a local scale was investigated. Thirty clones of phototrophic bacteria were isolated from each of five unevenly spaced sampling locations in freshwater marsh sediments along a linear 10-m transect, and a total of 150 clones were character...

journal_title：Applied and environmental microbiology

authors： Oda Y,Star B,Huisman LA,Gottschal JC,Forney LJ

更新日期：2003-09-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:Comparisons of 16S rDNA sequences were used to determine the phylogeny of not-yet-cultured spirochetes from hindguts of the African higher termite, Nasutitermes lujae (Wasmann). The 16S rRNA genes were amplified directly from spirochete-rich hindguts by using universal primers, and the amplified products were cloned i...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Dewhirst FE,Cooke SM,Fussing V,Poulsen LK,Breznak JA

更新日期：1996-02-01 00:00:00

abstract：:Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA extraction methods (DN...

journal_title：Applied and environmental microbiology

authors： Jiang J,Alderisio KA,Singh A,Xiao L

更新日期：2005-03-01 00:00:00

abstract：:Bacteriovorous protozoa harboring symbiotic algae are abundant in aquatic ecosystems, yet despite a recent interest in protozoan bacterivory, the influence of light on their ingestion rates has not been investigated. In this study, Paramecium bursaria containing endosymbiotic Chlorella was tested for the effect of lig...

journal_title：Applied and environmental microbiology

authors： Berk SG,Parks LH,Ting RS

更新日期：1991-08-01 00:00:00

abstract：:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

journal_title：Applied and environmental microbiology

authors： Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

更新日期：1992-08-01 00:00:00

abstract：:Nitrate, sulfate, and carbonate were used as electron acceptors to examine the anaerobic biodegradability of chlorinated aromatic compounds in estuarine and freshwater sediments. The respective denitrifying, sulfidogenic, and methanogenic enrichment cultures were established on each of the monochlorinated phenol and m...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Rivera MD,Young LY

更新日期：1993-04-01 00:00:00

abstract：:The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

journal_title：Applied and environmental microbiology

authors： Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

更新日期：2007-10-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00

abstract：:The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as...

journal_title：Applied and environmental microbiology

authors： Van Dijck P,van de Voorde H

更新日期：1976-03-01 00:00:00

abstract：:Hadal environments sustain diverse microorganisms. A few studies have investigated hadal microbial communities consisting of free-living or particle-associated bacteria and archaea. However, animal-associated microbial communities in hadal environments remain largely unexplored, and comparative analyses of animal gut ...

journal_title：Applied and environmental microbiology

authors： Zhang W,Watanabe HK,Ding W,Lan Y,Tian RM,Sun J,Chen C,Cai L,Li Y,Oguri K,Toyofuku T,Kitazato H,Drazen JC,Bartlett D,Qian PY

更新日期：2018-12-13 00:00:00

abstract：:Male-specific RNA coliphages (F-RNA coliphages) have been proposed as a potential viral indicator of fecal contamination in water and foods because they are easy to culture and are a normal component of the mammalian gut flora. F-RNA coliphage plaque numbers are typically obtained by directly plating a 10-fold dilutio...

journal_title：Applied and environmental microbiology

authors： Jones TH,Johns MW

更新日期：2009-10-01 00:00:00

abstract：:The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid o...

journal_title：Applied and environmental microbiology

authors： Koyama Y,Okamoto S,Furukawa K

更新日期：1990-07-01 00:00:00