Stability of the delta-endotoxin gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis.

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:Two subpopulations of Bacillus megaterium spores (1.360 and 1.355 g/ml) were obtained by density gradient centrifugation. The heavier spores had a higher thermoresistance (e.g., D(80) = 186 versus 81 min) and a higher DNA content (1.25 x 10 versus 0.65 x 10 g per spore, apparently corresponding to digenomic versus mon...

journal_title：Applied and environmental microbiology

authors： Belliveau BH,Beaman TC,Gerhardt P

更新日期：1990-09-01 00:00:00

abstract：:Fusarium fungal contaminants and related mycotoxins were investigated in eight maize feed samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. Fusarium moniliforme, F. proliferatum, and F. subglutinans were isolated from seven, eight, and five samples, respectively. These strains belonged t...

journal_title：Applied and environmental microbiology

authors： Munkvold G,Stahr HM,Logrieco A,Moretti A,Ritieni A

更新日期：1998-10-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:There have been few reports on the epidemiological analysis of environmental Leptospira isolates. This is probably because the isolation of leptospires from the environment was usually unsuccessful due to the overgrowth of contaminants and the slow growth of Leptospira. In this study, we collected a total of 88 sample...

journal_title：Applied and environmental microbiology

authors： Saito M,Villanueva SY,Chakraborty A,Miyahara S,Segawa T,Asoh T,Ozuru R,Gloriani NG,Yanagihara Y,Yoshida S

更新日期：2013-01-01 00:00:00

abstract：:A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Valster RM,Wullings BA,Boonstra H,Smidt H,van der Kooij D

更新日期：2006-09-01 00:00:00

abstract：:We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of ap...

journal_title：Applied and environmental microbiology

authors： Stepkowski T,Moulin L,Krzyzańska A,McInnes A,Law IJ,Howieson J

更新日期：2005-11-01 00:00:00

abstract：:Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

journal_title：Applied and environmental microbiology

authors： Schroeder LL,Bullerman LB

更新日期：1985-10-01 00:00:00

abstract：:The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, res...

journal_title：Applied and environmental microbiology

authors： Duff SJ,Cooper DG,Fuller OM

更新日期：1985-04-01 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

journal_title：Applied and environmental microbiology

authors： Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

更新日期：1999-11-01 00:00:00

abstract：:In two intertidal marshes, the vertical distribution in the sediment and inhibition by ammonium of methane oxidation were investigated by slurry incubation experiments. The two sites differ in their dominant vegetation type, i.e., reed and bulrush, and in their heights above sea level. The reed site was elevated with ...

journal_title：Applied and environmental microbiology

authors： Van Der Nat F,De Brouwer J,Middelburg JJ,Laanbroek HJ

更新日期：1997-12-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:Glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. By using the genome sequence and expressed sequence tag data available for Aspergillus oryzae RIB40, we cloned a novel glutaminase gene...

journal_title：Applied and environmental microbiology

authors： Ito K,Matsushima K,Koyama Y

更新日期：2012-08-01 00:00:00

abstract：:Propionate-forming bacteria seem to be abundant in anoxic rice paddy soil, but biogeochemical investigations show that propionate is not a correspondingly important intermediate in carbon flux in this system. Mixed cultures of Opitutus terrae strain PB90-1, a representative propionate-producing bacterium from rice pad...

journal_title：Applied and environmental microbiology

authors： Chin KJ,Janssen PH

更新日期：2002-04-01 00:00:00

abstract：:Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activit...

journal_title：Applied and environmental microbiology

authors： Mayer VW,Goin CJ

更新日期：1983-02-01 00:00:00

abstract：:POL-88, a mutant of the white-rot fungus Phanerochaete chrysosporium, was selected for diminished phenol-oxidizing enzyme activity. A wide variety of phenolic compounds were degraded by ligninolytic cultures of this mutant. With several o-diphenolic substrates, degradation intermediates were produced that had UV spect...

journal_title：Applied and environmental microbiology

authors： Leatham GF,Crawford RL,Kirk TK

更新日期：1983-07-01 00:00:00

abstract：:In order to identify antifungal compounds from natural sources to be used as ingredients in the bakery industry, water/salt-soluble extracts (WSE) from different legume flour hydrolysates obtained by the use of a fungal protease were assayed against Penicillium roqueforti DPPMAF1. The agar diffusion assays allowed the...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,Lavecchia A,Gramaglia V,Gobbetti M

更新日期：2015-06-15 00:00:00

abstract：:We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Apajalahti JH,Salkinoja-Salonen MS

更新日期：1988-03-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...

journal_title：Applied and environmental microbiology

authors： Sela S,Nestel D,Pinto R,Nemny-Lavy E,Bar-Joseph M

更新日期：2005-07-01 00:00:00

abstract：UNLABELLED:Our goal was to develop a robust tagging method that can be used to track bacterial strains in vivo To address this challenge, we adapted two existing systems: a modular plasmid-based reporter system (pCS26) that has been used for high-throughput gene expression studies in Salmonella and Escherichia coli and...

journal_title：Applied and environmental microbiology

authors： Shivak DJ,MacKenzie KD,Watson NL,Pasternak JA,Jones BD,Wang Y,DeVinney R,Wilson HL,Surette MG,White AP

更新日期：2016-07-29 00:00:00

abstract：:The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

journal_title：Applied and environmental microbiology

authors： Casiano-Colón A,Marquis RE

更新日期：1988-06-01 00:00:00

abstract：:The transposon Tn916 and self-mobilizing plasmid pAM beta 1 were conjugated from Enterococcus faecalis to the ruminal bacterium Streptococcus bovis JB1. Transconjugants were identified by resistance to tetracycline (Tn916) or erythromycin (pAM beta 1) and by Southern hybridization analyses. Transfer frequencies were 7...

journal_title：Applied and environmental microbiology

authors： Hespell RB,Whitehead TR

更新日期：1991-09-01 00:00:00

abstract：:Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

journal_title：Applied and environmental microbiology

authors： Zhang L,Sun C

更新日期：2018-08-31 00:00:00

abstract：:The respiratory and photosynthetic quinones of microbial mats which occurred in Japanese sulfide-containing neutral-pH hot springs at different temperatures were analyzed by spectrochromatography and mass spectrometry. All of the microbial mats that developed at high temperatures (temperatures above 68 degreesC) were ...

journal_title：Applied and environmental microbiology

authors： Hiraishi A,Umezawa T,Yamamoto H,Kato K,Maki Y

更新日期：1999-01-01 00:00:00

abstract：:Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

journal_title：Applied and environmental microbiology

authors： Schmidt T,Stoppel RD,Schlegel HG

更新日期：1991-11-01 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00

abstract：:The distribution of Borrelia burgdorferi sensu lato genospecies in questing Ixodes ricinus ticks from ecologically distinct habitats in Latvia was analyzed. A significant variation in the frequency of the genospecies across sites was observed, pointing to the importance of the host community in the ecology of Lyme bor...

journal_title：Applied and environmental microbiology

authors： Etti S,Hails R,Schäfer SM,De Michelis S,Sewell HS,Bormane A,Donaghy M,Kurtenbach K

更新日期：2003-05-01 00:00:00

abstract：:Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine beta-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recom...

journal_title：Applied and environmental microbiology

authors： Bernasconi E,Germond JE,Delley M,Fritsché R,Corthésy B

更新日期：2002-06-01 00:00:00