Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1.

abstract：:pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred from Streptococcus lactis to Bacillus subtilis. No conjugal transfer of pAM beta 1 from B. subtilis to S. lactis was observed. In addition, pAM beta 1 which had been reintroduced in S. lactis after cycling t...

journal_title：Applied and environmental microbiology

authors： van der Lelie D,Venema G

更新日期：1987-10-01 00:00:00

abstract：:The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

journal_title：Applied and environmental microbiology

authors： Oh HM,Lee SJ,Jang MH,Yoon BD

更新日期：2000-01-01 00:00:00

abstract：:Anaerobic degradation of monochlorophenols and monochlorobenzoates in a variety of aquatic sediments was compared under four enrichment conditions. A broader range of compounds was degraded in enrichments inoculated with sediment exposed to industrial effluents. Degradation of chloroaromatic compounds was observed mos...

journal_title：Applied and environmental microbiology

authors： Genthner BR,Price WA,Pritchard PH

更新日期：1989-06-01 00:00:00

abstract：:Eukaryotic marine microbes play pivotal roles in biogeochemical nutrient cycling and ecosystem function, but studies that focus on the protistan biogeography and genetic diversity lag-behind studies of other microbes. 18S rRNA PCR amplification and clone library sequencing are commonly used to assess diversity that is...

journal_title：Applied and environmental microbiology

authors： Koid A,Nelson WC,Mraz A,Heidelberg KB

更新日期：2012-06-01 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:The broad-host-range (BHR) conjugative plasmids have developed diverse adaptive mechanisms defining the range of their promiscuity. The BHR conjugative RA3 plasmid, the archetype of the IncU group, can transfer between, replicate in, and be maintained in representatives of Alpha-, Beta-, and Gammaproteobacteria Its st...

journal_title：Applied and environmental microbiology

authors： Lewicka E,Dolowy P,Godziszewska J,Litwin E,Ludwiczak M,Jagura-Burdzy G

更新日期：2020-08-03 00:00:00

abstract：:Human sapovirus sequences were identified in 12 (100%) influent and 7 (58%) effluent wastewater samples collected once a month for a year. The strains were characterized based on their partial capsid gene sequences and classified into 10 genotypes, demonstrating that genetically diverse sapovirus strains infect humans...

journal_title：Applied and environmental microbiology

authors： Kitajima M,Haramoto E,Phanuwan C,Katayama H

更新日期：2011-06-01 00:00:00

abstract：:The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h(-1) for 30 min and 25 mm h(-1) for 180 min were applied to soil plots with artificial fecal pats seeded with app...

journal_title：Applied and environmental microbiology

authors： Davies CM,Ferguson CM,Kaucner C,Krogh M,Altavilla N,Deere DA,Ashbolt NJ

更新日期：2004-02-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:During the fermentation of sake, cells of Saccharomyces cerevisiae are exposed to high concentrations of ethanol, thereby damaging the cell membrane and functional proteins. L-proline protects yeast cells from damage caused by freezing or oxidative stress. In this study, we evaluated the role of intracellular L-prolin...

journal_title：Applied and environmental microbiology

authors： Takagi H,Takaoka M,Kawaguchi A,Kubo Y

更新日期：2005-12-01 00:00:00

abstract：:Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

journal_title：Applied and environmental microbiology

authors： Thomas PM,Golly KF,Virginia RA,Zyskind JW

更新日期：1995-09-01 00:00:00

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:Actinomycetes secrete into their surroundings a suite of enzymes involved in the biodegradation of plant lignocellulose; these have been reported to include both hydrolytic and oxidative enzymes, including peroxidases. Reports of secreted peroxidases have been based upon observations of peroxidase-like activity associ...

journal_title：Applied and environmental microbiology

authors： Mason MG,Ball AS,Reeder BJ,Silkstone G,Nicholls P,Wilson MT

更新日期：2001-10-01 00:00:00

abstract：:To study the anaerobic degradation of the chimera 3-chloro-4-hydroxybenzoate (3-Cl,4-OHB), anaerobic freshwater sediment samples from the vicinity of Athens, Ga., were adapted for the transformation of 4-hydroxybenzoate (4-OHB), 3-chlorobenzoate (3-CB), 2-chlorophenol (2-CP), and 2,4-dichlorophenol (2,4-DCP). In nonad...

journal_title：Applied and environmental microbiology

authors： Zhang X,Wiegel J

更新日期：1992-11-01 00:00:00

abstract：:In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...

journal_title：Applied and environmental microbiology

authors： Zhang Q,Nghiem J,Silverberg GJ,Vecitis CD

更新日期：2015-07-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:Three species of saltmarsh ascomycetes were grown in the presence of all of the constituents of their natural substrate (leaves of cordgrass) or were presented only with aqueous extracts of the leaves. These two growth-condition treatments had no significant effect on total ergosterol content of the fungal mycelia, co...

journal_title：Applied and environmental microbiology

authors： Newell SY

更新日期：1994-09-01 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the ...

journal_title：Applied and environmental microbiology

authors： Armstrong JL,Calomiris JJ,Seidler RJ

更新日期：1982-08-01 00:00:00

abstract：:A highly sensitive and specific PCR-based method of monitoring 16S rRNA genes of Pseudomonas stutzeri was developed for searching P. stutzeri DNA in environmental samples. This monitoring was combined with a reliable and sensitive method for isolating P. stutzeri colony formers from soil and sediment, depending on the...

journal_title：Applied and environmental microbiology

authors： Sikorski J,Teschner N,Wackernagel W

更新日期：2002-02-01 00:00:00

abstract：:Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular parasite of aquatic amoebae and human macrophages. A key factor for L. pneumophila in intracellular infection is its type II protein secretion system (Lsp). In order to more completely define Lsp output, we recently performed a proteomic a...

journal_title：Applied and environmental microbiology

authors： Rossier O,Dao J,Cianciotto NP

更新日期：2008-02-01 00:00:00

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:Juvenile oyster disease (JOD) causes significant annual mortalities of hatchery-produced Eastern oysters, Crassostrea virginica, cultured in the Northeast. We have reported that a novel species of the alpha-proteobacteria Roseobacter group (designated CVSP) was numerically dominant in JOD-affected animals sampled duri...

journal_title：Applied and environmental microbiology

authors： Boettcher KJ,Barber BJ,Singer JT

更新日期：2000-09-01 00:00:00

abstract：:A model conditional-suicide system to control genetically engineered microorganisms able to degrade substituted benzoates is reported. The system is based on two elements. One element consists of a fusion between the promoter of the Pseudomonas putida TOL plasmid-encoded meta-cleavage pathway operon (P(m)) and the lac...

journal_title：Applied and environmental microbiology

authors： Contreras A,Molin S,Ramos JL

更新日期：1991-05-01 00:00:00

abstract：:Listeria monocytogenes secretes two chitinases and one chitin binding protein. Mutants lacking chiA, chiB, or lmo2467 exhibited normal growth in cultured cells but were defective for growth in the livers and spleens of mice. Mammals lack chitin; thus, L. monocytogenes may have adapted chitinases to recognize alternati...

journal_title：Applied and environmental microbiology

authors： Chaudhuri S,Bruno JC,Alonzo F 3rd,Xayarath B,Cianciotto NP,Freitag NE

更新日期：2010-11-01 00:00:00

abstract：:ATP-dependent phosphorylation of [14C]galactose by 11 strains of Streptococcus thermophilus indicated that these organisms possessed the Leloir enzyme, galactokinase (galK). Activities were 10 times higher in fully induced, galactose-fermenting (Gal+) strains than in galactose-nonfermenting (Gal-) strains. Lactose-gro...

journal_title：Applied and environmental microbiology

authors： Hutkins R,Morris HA,McKay LL

更新日期：1985-10-01 00:00:00

abstract：:A bacterium capable of anaerobic growth via reductive dehalogenation of 2-chlorophenol was isolated from a culture enriched from sediment taken from a small stream near Lansing, Mich. The organism, designated strain 2CP-1, is a gram-negative rod ca. 3 by 0.5 micron in size and is a catalase-negative, oxidase-negative,...

journal_title：Applied and environmental microbiology

authors： Cole JR,Cascarelli AL,Mohn WW,Tiedje JM

更新日期：1994-10-01 00:00:00

abstract：:Two methods, digestion and elution, were used to recover parasitic nematodes from 470 flatfish belonging to species in the family Pleuronectidae. Samples of similar fish were collected from market lots; half of each sample was subjected to digestion, and half was subjected to elution (sedimentation). The edible (flesh...

journal_title：Applied and environmental microbiology

authors： Jackson GJ,Bier JW,Payne WL,McClure FD

更新日期：1981-04-01 00:00:00

abstract：:Virulence gene profiles of atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) from cattle, sheep, and humans were examined to determine the relationship between pathotypes. Shared virulence factors (intimin, EHEC hemolysin, serine protease, and a type II secretion system) were ...

journal_title：Applied and environmental microbiology

authors： Cookson AL,Cao M,Bennett J,Nicol C,Thomson-Carter F,Attwood GT

更新日期：2010-06-01 00:00:00