Impacts of hematite nanoparticle exposure on biomechanical, adhesive, and surface electrical properties of Escherichia coli cells.

Abstract:

:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electrical properties of Escherichia coli cells with and without exposure to hematite nanoparticles (NPs) in an effort to advance our understanding of the cytotoxic impacts of nanomaterials. Both scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that E. coli cells had noticeable deformation with hematite treatment for 45 min with a statistical significance. The hematite-treated cells became significantly harder or stiffer than untreated ones, as evidenced by indentation and spring constant measurements. The average indentation of the hematite-treated E. coli cells was 120 nm, which is significantly lower (P < 0.01) than that of the untreated cells (approximately 400 nm). The spring constant of hematite-treated E. coli cells (0.28 ± 0.11 nN/nm) was about 20 times higher than that of untreated ones (0.01 ± 0.01 nN/nm). The zeta potential of E. coli cells, measured by dynamic light scattering (DLS), was shown to shift from -4 ± 2 mV to -27 ± 8 mV with progressive surface adsorption of hematite NPs, a finding which is consistent with the local surface potential measured by Kelvin probe force microscopy (KPFM). Overall, the reported findings quantitatively revealed the adverse impacts of nanomaterial exposure on physical properties of bacterial cells and should provide insight into the toxicity mechanisms of nanomaterials.

journal_name

Appl Environ Microbiol

authors

Zhang W,Hughes J,Chen Y

doi

10.1128/AEM.00193-12

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

3905-15

issue

11

eissn

0099-2240

issn

1098-5336

pii

AEM.00193-12

journal_volume

78

pub_type

杂志文章
  • Stereochemistry of furfural reduction by a Saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification.

    abstract::Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00542-10

    authors: Bowman MJ,Jordan DB,Vermillion KE,Braker JD,Moon J,Liu ZL

    更新日期:2010-08-01 00:00:00

  • Direct assessment of viral diversity in soils by random PCR amplification of polymorphic DNA.

    abstract::Viruses are the most abundant and diverse biological entities within soils, yet their ecological impact is largely unknown. Defining how soil viral communities change with perturbation or across environments will contribute to understanding the larger ecological significance of soil viruses. A new approach to examinin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00268-13

    authors: Srinivasiah S,Lovett J,Polson S,Bhavsar J,Ghosh D,Roy K,Fuhrmann JJ,Radosevich M,Wommack KE

    更新日期:2013-09-01 00:00:00

  • Biotransformation of linoleic acid and bile acids by Eubacterium lentum.

    abstract::Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. M...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.1.39-43.1984

    authors: Eyssen H,Verhulst A

    更新日期:1984-01-01 00:00:00

  • Regulation of aflR and its product, AflR, associated with aflatoxin biosynthesis.

    abstract::We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3718-3723.1998

    authors: Liu BH,Chu FS

    更新日期:1998-10-01 00:00:00

  • Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1.

    abstract::A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3481-3486.2000

    authors: Ishige T,Tani A,Sakai Y,Kato N

    更新日期:2000-08-01 00:00:00

  • Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32.

    abstract::A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.9.3411-3415.1998

    authors: Chen YS,Steele JL

    更新日期:1998-09-01 00:00:00

  • Hemolymph Microbiomes of Three Aquatic Invertebrates as Revealed by a New Cell Extraction Method.

    abstract::Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimp, and oysters. Hemolymph is a critical site in the host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02824-17

    authors: Zhang X,Sun Z,Zhang X,Zhang M,Li S

    更新日期:2018-04-02 00:00:00

  • Fluorogenic selective and differential medium for isolation of fecal streptococci.

    abstract::Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.2.622-627.1983

    authors: Littel KJ,Hartman PA

    更新日期:1983-02-01 00:00:00

  • Coliform aerosols generated from the surface of dewatered sewage applied to a forest clearcut.

    abstract::Concentrations of airborne coliform bacteria as high as 1.5 X 10(4) m-3 were observed 8 cm above anaerobically digested sewage sludge applied to a forest clearcut. Dry conditions and high wind speeds tended to favor aerosol generation. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.6.972-974.1978

    authors: Edmonds RL,Littke W

    更新日期:1978-12-01 00:00:00

  • Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485.

    abstract::Two acetyl esterases (EC 3.1.1.6) were purified to gel electrophoretic homogeneity from Thermoanaerobacterium sp. strain JW/SL-YS485, an anaerobic, thermophilic endospore former which is able to utilize various substituted xylans for growth. Both enzymes released acetic acid from chemically acetylated larch xylan. Ace...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.2.729-733.1995

    authors: Shao W,Wiegel J

    更新日期:1995-02-01 00:00:00

  • Molecular analysis of shower curtain biofilm microbes.

    abstract::Households provide environments that encourage the formation of microbial communities, often as biofilms. Such biofilms constitute potential reservoirs for pathogens, particularly for immune-compromised individuals. One household environment that potentially accumulates microbial biofilms is that provided by vinyl sho...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.4187-4192.2004

    authors: Kelley ST,Theisen U,Angenent LT,St Amand A,Pace NR

    更新日期:2004-07-01 00:00:00

  • Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species.

    abstract::Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are chara...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02768-06

    authors: McMurdie PJ,Behrens SF,Holmes S,Spormann AM

    更新日期:2007-04-01 00:00:00

  • Intestinal colonization potential of turbot (Scophthalmus maximus)- and dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum.

    abstract::Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen Vibrio anguillarum. The aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.2.551-556.1992

    authors: Olsson JC,Westerdahl A,Conway PL,Kjelleberg S

    更新日期:1992-02-01 00:00:00

  • 19F nuclear magnetic resonance analysis of 5-fluorouracil metabolism in wild-type and 5-fluorouracil-resistant Nectria haematococca.

    abstract::A mutant (furA3) was isolated from the S1 wild-type strain of Nectria haematococca on the basis of its resistance to 5-fluorouracil (5FU). This mutant has greatly reduced activity of uracil phosphoribosyltransferase, a pyrimidine salvage enzyme catalyzing the synthesis of UMP from uracil. The metabolism of 5FU was exa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.10.2474-2479.1989

    authors: Parisot D,Malet-Martino MC,Crasnier P,Martino R

    更新日期:1989-10-01 00:00:00

  • Prevalence of Ca²⁺-ATPase-mediated carbonate dissolution among cyanobacterial euendoliths.

    abstract::Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06633-11

    authors: Ramírez-Reinat EL,Garcia-Pichel F

    更新日期:2012-01-01 00:00:00

  • Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.

    abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00239-15

    authors: Maisuria VB,Hosseinidoust Z,Tufenkji N

    更新日期:2015-06-01 00:00:00

  • Methods for integrated air sampling and dna analysis for detection of airborne fungal spores.

    abstract::Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2453-2459.2001

    authors: Williams RH,Ward E,McCartney HA

    更新日期:2001-06-01 00:00:00

  • Microbial community composition and denitrifying enzyme activities in salt marsh sediments.

    abstract::Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01221-08

    authors: Cao Y,Green PG,Holden PA

    更新日期:2008-12-01 00:00:00

  • Dual substrate specificity of an N-acetylglucosamine phosphotransferase system in Clostridium beijerinckii.

    abstract::The solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. In common with other anaerobes, the clostridia show a marked dependence on the phosphoeno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01866-13

    authors: Al Makishah NH,Mitchell WJ

    更新日期:2013-11-01 00:00:00

  • Commercially distributed meat as a potential vehicle for community-acquired methicillin-resistant Staphylococcus aureus.

    abstract::The incidence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection has been increasing; however, the sources of infection remain unclear. Therefore, we investigated the involvement of meat as a possible mediator of CA-MRSA infection. We examined the distribution of MRSA strains in comm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07470-11

    authors: Ogata K,Narimatsu H,Suzuki M,Higuchi W,Yamamoto T,Taniguchi H

    更新日期:2012-04-01 00:00:00

  • Molecular view by fourier transform infrared spectroscopy of the relationship between lactocin 705 and membranes: speculations on antimicrobial mechanism.

    abstract::Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01293-06

    authors: Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

    更新日期:2007-01-01 00:00:00

  • Rapid growth of planktonic Vibrio cholerae non-O1/non-O139 strains in a large alkaline lake in Austria: dependence on temperature and dissolved organic carbon quality.

    abstract::Vibrio cholerae non-O1/non-O139 strains have caused several cases of ear, wound, and blood infections, including one lethal case of septicemia in Austria, during recent years. All of these cases had a history of local recreational activities in the large eastern Austrian lake Neusiedler See. Thus, a monitoring program...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01739-07

    authors: Kirschner AK,Schlesinger J,Farnleitner AH,Hornek R,Süss B,Golda B,Herzig A,Reitner B

    更新日期:2008-04-01 00:00:00

  • A lithium-sensitive and sodium-tolerant 3'-phosphoadenosine-5'-phosphatase encoded by halA from the cyanobacterium Arthrospira platensis is closely related to its counterparts from yeasts and plants.

    abstract::3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.245-251.2006

    authors: Zhang JY,Zou J,Bao Q,Chen WL,Wang L,Yang H,Zhang CC

    更新日期:2006-01-01 00:00:00

  • Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones.

    abstract::More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5248-5254.2003

    authors: Hahn MW

    更新日期:2003-09-01 00:00:00

  • A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase.

    abstract::The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7140-7147.2004

    authors: Vellore J,Moretz SE,Lampson BC

    更新日期:2004-12-01 00:00:00

  • Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses.

    abstract::Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4200-4204.2003

    authors: Halpern M,Gancz H,Broza M,Kashi Y

    更新日期:2003-07-01 00:00:00

  • Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.

    abstract::Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.680-685.1999

    authors: Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

    更新日期:1999-02-01 00:00:00

  • Epidemiological association of different Campylobacter jejuni groups with metabolism-associated genetic markers.

    abstract::In this study, multilocus sequence typing (MLST) was combined with the genetic detection of six genetic markers, ansB, dmsA, ggt, cj1585c, cjj81176-1367/71 (cj1365c), and the two-gene marker tlp7 (cj0951c plus cj0952c), to assess if their presence correlated with different C. jejuni clonal groups. Using a collection o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02403-10

    authors: Zautner AE,Herrmann S,Corso J,Tareen AM,Alter T,Gross U

    更新日期:2011-04-01 00:00:00

  • Effect of spatial differences in microbial activity, pH, and substrate levels on methanogenesis initiation in refuse.

    abstract::The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02349-10

    authors: Staley BF,de Los Reyes FL 3rd,Barlaz MA

    更新日期:2011-04-01 00:00:00

  • Comparative Serology of the Marine Fish Pathogen Vibrio anguillarum.

    abstract::The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4367-4373.1995

    authors: Grisez L,Ollevier F

    更新日期:1995-12-01 00:00:00