Abstract:
:Vibrio cholerae non-O1/non-O139 strains have caused several cases of ear, wound, and blood infections, including one lethal case of septicemia in Austria, during recent years. All of these cases had a history of local recreational activities in the large eastern Austrian lake Neusiedler See. Thus, a monitoring program was started to investigate the prevalence of V. cholerae strains in the lake over several years. Genetic analyses of isolated strains revealed the presence of a variety of pathogenic genes, but in no case did we detect the cholera toxin gene or the toxin-coregulated pilus gene, both of which are prerequisites for the pathogen to be able to cause cholera. In addition, experiments were performed to elucidate the preferred ecological niche of this pathogen. As size filtration experiments indicated and laboratory microcosms showed, endemic V. cholerae could rapidly grow in a free-living state in natural lake water at growth rates similar to those of the bulk natural bacterial population. Temperature and the quality of dissolved organic carbon had a highly significant influence on V. cholerae growth. Specific growth rates, growth yield, and enzyme activity decreased markedly with increasing concentrations of high-molecular-weight substances, indicating that the humic substances originating from the extensive reed belt in the lake can inhibit V. cholerae growth.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kirschner AK,Schlesinger J,Farnleitner AH,Hornek R,Süss B,Golda B,Herzig A,Reitner Bdoi
10.1128/AEM.01739-07subject
Has Abstractpub_date
2008-04-01 00:00:00pages
2004-15issue
7eissn
0099-2240issn
1098-5336pii
AEM.01739-07journal_volume
74pub_type
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pub_type: 杂志文章
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