Molecular view by fourier transform infrared spectroscopy of the relationship between lactocin 705 and membranes: speculations on antimicrobial mechanism.

abstract：:Two alpha-amylase genes from the thermophilic alkaliphile Anaerobranca gottschalkii were cloned, and the corresponding enzymes, AmyA and AmyB, were investigated after purification of the recombinant proteins. Based on their amino acid sequences, AmyA is proposed to be a lipoprotein with extracellular localization and ...

journal_title：Applied and environmental microbiology

authors： Ballschmiter M,Armbrecht M,Ivanova K,Antranikian G,Liebl W

更新日期：2005-07-01 00:00:00

abstract：:Bacillus thuringiensis three-domain Cry toxins kill insects by forming pores in the apical membrane of larval midgut cells. Oligomerization of the toxin is an important step for pore formation. Domain I helix α-3 participates in toxin oligomerization. Here we identify an intramolecular salt bridge within helix α-3 of ...

journal_title：Applied and environmental microbiology

authors： Pacheco S,Gómez I,Sánchez J,García-Gómez BI,Soberón M,Bravo A

更新日期：2017-09-29 00:00:00

abstract：:A total of 530 strains of Staphylococcus aureus were isolated from the defeathering machinery of a chicken processing plant and from neck skin samples of carcasses at different stages of processing in two visits 4 weeks apart. Eleven different plasmid profiles were detected in the isolates, eight being common to both ...

journal_title：Applied and environmental microbiology

authors： Dodd CE,Chaffey BJ,Waites WM

更新日期：1988-06-01 00:00:00

abstract：:Growth and succinate versus lactate production from glucose by Anaerobiospirillum succiniciproducens was regulated by the level of available carbon dioxide and culture pH. At pH 7.2, the generation time was almost doubled and extensive amounts of lactate were formed in comparison with growth at pH 6.2. The succinate y...

journal_title：Applied and environmental microbiology

authors： Samuelov NS,Lamed R,Lowe S,Zeikus JG

更新日期：1991-10-01 00:00:00

abstract：:Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

journal_title：Applied and environmental microbiology

authors： Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

更新日期：1998-08-01 00:00:00

abstract：:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

journal_title：Applied and environmental microbiology

authors： Begley M,Cotter PD,Hill C,Ross RP

更新日期：2010-10-01 00:00:00

abstract：:Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the development of host-specific ...

journal_title：Applied and environmental microbiology

authors： Lu J,Santo Domingo JW,Hill S,Edge TA

更新日期：2009-09-01 00:00:00

abstract：:The undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of Listeria spp. In some cases, these microbial consortia inhibit Listeria almost completely. From such undefined industrial cheese-ripening floras, ...

journal_title：Applied and environmental microbiology

authors： Eppert I,Valdés-Stauber N,Götz H,Busse M,Scherer S

更新日期：1997-12-01 00:00:00

abstract：:Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-gener...

journal_title：Applied and environmental microbiology

authors： Martínez JM,Martínez MI,Suárez AM,Herranz C,Casaus P,Cintas LM,Rodríguez JM,Hernández PE

更新日期：1998-11-01 00:00:00

abstract：:Perchlorate contamination can be microbially respired to innocuous chloride and thus can be treated effectively. However, monitoring a bioremediative strategy is often difficult due to the complexities of environmental samples. Here we demonstrate that microbial respiration of perchlorate results in a significant frac...

journal_title：Applied and environmental microbiology

authors： Coleman ML,Ader M,Chaudhuri S,Coates JD

更新日期：2003-08-01 00:00:00

abstract：:The microbial transformation of [N-methyl-(sup14)C]aldicarb, a carbamate pesticide, occurred in aquifer, lake, and salt marsh sediments. Microbial degradation of aldicarb took place within 21 days in aquifer sediments from sites previously exposed to aldicarb (Jamesport, Long Island, N.Y.) but did not occur in sedimen...

journal_title：Applied and environmental microbiology

authors： Kazumi J,Capone DG

更新日期：1995-08-01 00:00:00

abstract：:Laboratory-grown strains of chemoautotrophic Thiomicrospira sp. strain L-12 and Thiobacillus neapolitanus produced cell carbon that was 24.6 to 25.1 ppt (24.6 to 25.1 mg/g) lower in C isotope abundance than the ambient source of carbon dioxide and bicarbonate. This degree of C isotope depletion was comparable to that ...

journal_title：Applied and environmental microbiology

authors： Ruby EG,Jannasch HW,Deuser WG

更新日期：1987-08-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...

journal_title：Applied and environmental microbiology

authors： Naranjo CA,Jivan AD,Vo MN,de Sa Campos KH,Deyarmin JS,Hekman RM,Uribe C,Hang A,Her K,Fong MM,Choi JJ,Chou C,Rabara TR,Myers G,Moua P,Thor,Risser DD,Vierra CA,Franz AH,Lin-Cereghino J,Lin-Cereghino GP

更新日期：2019-11-27 00:00:00

abstract：:An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

journal_title：Applied and environmental microbiology

authors： Coleman NV,Spain JC

更新日期：2003-10-01 00:00:00

abstract：:Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

journal_title：Applied and environmental microbiology

authors： Atlas RM,Williams JF,Huntington MK

更新日期：1996-04-01 00:00:00

abstract：:Thiobacillus ferrooxidans is an acidophilic organism important to metal leaching of low-grade ores. The aforementioned importance is related to the ability of the bacterium to oxidize reduced iron and sulfur, principally found in nature as pyrite (FeS2). The present study dealt with sulfide oxidation at low pH values ...

journal_title：Applied and environmental microbiology

authors： Tano T,Lundgren D

更新日期：1978-06-01 00:00:00

abstract：:Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

journal_title：Applied and environmental microbiology

authors： Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

更新日期：1987-07-01 00:00:00

abstract：:The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity to several well-chara...

journal_title：Applied and environmental microbiology

authors： Cai G,Zhu S,Yang S,Zhao G,Jiang W

更新日期：2004-05-01 00:00:00

abstract：:The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microco...

journal_title：Applied and environmental microbiology

authors： Heitkamp MA,Freeman JP,Cerniglia CE

更新日期：1986-02-01 00:00:00

abstract：:Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(...

journal_title：Applied and environmental microbiology

authors： Vernozy-Rozand C,Montet MP,Bertin Y,Trably F,Girardeau JP,Martin C,Livrelli V,Beutin L

更新日期：2004-04-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:Candida parapsilosis, C. tropicalis, and Torulopsis glabrata were the human-associated yeasts most frequently isolated from quahogs, oysters, and mussels collected from four estuarine areas along the northern shore of Long Island Sound. Some inconsistency and seasonal variation in the occurrence of these and other yea...

journal_title：Applied and environmental microbiology

authors： Buck JD,Bubucis PM,Combs TJ

更新日期：1977-02-01 00:00:00

abstract：:We investigated associations of species of the Bacteroides fragilis group with Japanese cedar pollinosis (JCPsis). Cell numbers of Bacteroides fragilis and Bacteroides intestinalis were significantly higher in JCPsis subjects than in non-JCPsis subjects before the pollen season. They correlated positively with both sy...

journal_title：Applied and environmental microbiology

authors： Odamaki T,Xiao JZ,Sakamoto M,Kondo S,Yaeshima T,Iwatsuki K,Togashi H,Enomoto T,Benno Y

更新日期：2008-11-01 00:00:00

abstract：:Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of...

journal_title：Applied and environmental microbiology

authors： Powell JE,Martinson VG,Urban-Mead K,Moran NA

更新日期：2014-12-01 00:00:00

abstract：:Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize tr...

journal_title：Applied and environmental microbiology

authors： Carvalho AL,Cardoso FS,Bohn A,Neves AR,Santos H

更新日期：2011-06-01 00:00:00

abstract：:The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...

journal_title：Applied and environmental microbiology

authors： Dombek PE,Johnson LK,Zimmerley ST,Sadowsky MJ

更新日期：2000-06-01 00:00:00

abstract：:Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid culture...

journal_title：Applied and environmental microbiology

authors： Fang W,Leng B,Xiao Y,Jin K,Ma J,Fan Y,Feng J,Yang X,Zhang Y,Pei Y

更新日期：2005-01-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

journal_title：Applied and environmental microbiology

authors： Haines JR,Covert TC,Rankin CC

更新日期：1993-08-01 00:00:00

abstract：:Simulation of a heat process used in the terminal dry-heat decontamination of the Viking spacecraft is reported. Naturally occurring airborne bacterial spores were collected on Teflon ribbons in selected spacecraft assembly areas and subsequently subjected to dry heat. Thermal inactivation experiments were conducted a...

journal_title：Applied and environmental microbiology

authors： Puleo JR,Bergstrom SL,Peeler JT,Oxborrow GS

更新日期：1978-09-01 00:00:00