A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase.

Abstract:

:The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the synthesis of full-length cDNAs is frequently inefficient, because the RT commonly used often produces truncated cDNAs. Synthesizing cDNA at higher temperatures, on the other hand, can provide a number of improvements. These include increasing the length of cDNA product, greater accuracy, and greater specificity during reverse transcription. Thus, an RT that remains stable and active at hot temperatures may produce better-quality cDNAs and improve the yield of full-length cDNAs. Described here is the discovery of a gene, designated trt, from the genome of the thermophilic bacterium Bacillus (Geobacillus) stearothermophilus strain 10. The gene codes for an open reading frame (ORF) similar to the ORFs encoded by group II introns found in bacteria. The gene was cloned and overexpressed in Escherichia coli, and its protein product was partially purified. Like the host organism, the Trt protein is a heat-stable protein with RT activity and can reverse transcribe RNA at temperatures as high as 75 degrees C.

journal_name

Appl Environ Microbiol

authors

Vellore J,Moretz SE,Lampson BC

doi

10.1128/AEM.70.12.7140-7147.2004

keywords:

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

7140-7

issue

12

eissn

0099-2240

issn

1098-5336

pii

70/12/7140

journal_volume

70

pub_type

杂志文章
  • Effect of tannic acid on the transcriptome of the soil bacterium Pseudomonas protegens Pf-5.

    abstract::Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03101-12

    authors: Lim CK,Penesyan A,Hassan KA,Loper JE,Paulsen IT

    更新日期:2013-05-01 00:00:00

  • Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds.

    abstract::A variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT), were reduced by hydrogen in the presence of enzyme preparations from Veillonella alkalescens. Consistent with the proposed reduction pathway, R-NO2 H2 leads to R-NO H2 leads to R-NHOH H2 leads to R-NH2, 3 mol of H2 was utilized per mol of nit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.6.949-958.1976

    authors: McCormick NG,Feeherry FE,Levinson HS

    更新日期:1976-06-01 00:00:00

  • Reduced sulfur in ashes and slags from the gasification of coals: availability for chemical and microbial oxidation.

    abstract::This study was initiated to determine if reduced sulfur contained in coal gasifier ash and slag was available for microbial and chemical oxidation because eventual large-quantity landfill disposal of these solid wastes is expected. Continuous application of distilled water to a column containing a high-sulfur-content ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.743-747.1983

    authors: Strayer RF,Davis EC

    更新日期:1983-03-01 00:00:00

  • Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium "Candidatus Pelagibacter ubique".

    abstract::SAR11 bacteria are small, heterotrophic, marine alphaproteobacteria found throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean conditions, although the adaptations required for life under those conditions are not well understood. To illuminate this issue, we used cryo-electron to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02807-16

    authors: Zhao X,Schwartz CL,Pierson J,Giovannoni SJ,McIntosh JR,Nicastro D

    更新日期:2017-01-17 00:00:00

  • Duodenal-mucosal bacteria associated with celiac disease in children.

    abstract::Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00869-13

    authors: Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

    更新日期:2013-09-01 00:00:00

  • Identification of glyA (encoding serine hydroxymethyltransferase) and its use together with the exporter ThrE to increase L-threonine accumulation by Corynebacterium glutamicum.

    abstract::L-threonine can be made by the amino acid-producing bacterium Corynebacterium glutamicum. However, in the course of this process, some of the L-threonine is degraded to glycine. We detected an aldole cleavage activity of L-threonine in crude extracts with an activity of 2.2 nmol min(-1) (mg of protein)(-1). In order t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.7.3321-3327.2002

    authors: Simic P,Willuhn J,Sahm H,Eggeling L

    更新日期:2002-07-01 00:00:00

  • Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions.

    abstract::While filamentous cyanobacteria play a crucial role in food web dynamics and biogeochemical cycling of many aquatic ecosystems around the globe, the knowledge regarding the phages infecting them is limited. Here, we describe the complete genome of the virulent cyanophage vB_AphaS-CL131 (here, CL 131), a Siphoviridae p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01311-18

    authors: Šulčius S,Šimoliūnas E,Alzbutas G,Gasiūnas G,Jauniškis V,Kuznecova J,Miettinen S,Nilsson E,Meškys R,Roine E,Paškauskas R,Holmfeldt K

    更新日期:2018-12-13 00:00:00

  • Anaerobic Degradation of Chloroaromatic Compounds in Aquatic Sediments under a Variety of Enrichment Conditions.

    abstract::Anaerobic degradation of monochlorophenols and monochlorobenzoates in a variety of aquatic sediments was compared under four enrichment conditions. A broader range of compounds was degraded in enrichments inoculated with sediment exposed to industrial effluents. Degradation of chloroaromatic compounds was observed mos...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.6.1466-1471.1989

    authors: Genthner BR,Price WA,Pritchard PH

    更新日期:1989-06-01 00:00:00

  • Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.

    abstract::Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03765-15

    authors: Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

    更新日期:2015-12-18 00:00:00

  • Impacts of hematite nanoparticle exposure on biomechanical, adhesive, and surface electrical properties of Escherichia coli cells.

    abstract::Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00193-12

    authors: Zhang W,Hughes J,Chen Y

    更新日期:2012-06-01 00:00:00

  • Oxygen requirements of the food spoilage yeast Zygosaccharomyces bailii in synthetic and complex media.

    abstract::Most yeast species can ferment sugars to ethanol, but only a few can grow in the complete absence of oxygen. Oxygen availability might, therefore, be a key parameter in spoilage of food caused by fermentative yeasts. In this study, the oxygen requirement and regulation of alcoholic fermentation were studied in batch c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2123-2128.2001

    authors: Rodrigues F,Côrte-Real M,Leão C,van Dijken JP,Pronk JT

    更新日期:2001-05-01 00:00:00

  • Effect of dietary carbohydrates on bacterial cholyltaurine hydrolase in poultry intestinal homogenates.

    abstract::The bile salt hydrolase activity in intestinal homogenates reflects composite activities of the gastrointestinal microbial consortia. We have proposed that specific transformations of conjugated bile acids by the intestinal microflora result in the production of metabolites which depress the growth of poultry. The inf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.337-342.1988

    authors: Feighner SD,Dashkevicz MP

    更新日期:1988-02-01 00:00:00

  • Stability of the delta-endotoxin gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis.

    abstract::Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.12.3522-3528.1991

    authors: Turner JT,Lampel JS,Stearman RS,Sundin GW,Gunyuzlu P,Anderson JJ

    更新日期:1991-12-01 00:00:00

  • Correlation of the capsular phenotype in Propionibacterium freudenreichii with the level of expression of gtf, a unique polysaccharide synthase-encoding gene.

    abstract::Many food-grade bacteria produce exopolysaccharides (EPS) that affect the texture of fermented food products and that may be involved in probiotic properties. Propionibacterium freudenreichii is a Gram-positive food-grade bacterium with reported probiotic capabilities that is widely used as starter in Swiss-type chees...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02591-09

    authors: Deutsch SM,Le Bivic P,Hervé C,Madec MN,LaPointe G,Jan G,Le Loir Y,Falentin H

    更新日期:2010-05-01 00:00:00

  • iTRAQ-Based Global Proteomic Analysis of Salmonella enterica Serovar Typhimurium in Response to Desiccation, Low Water Activity, and Thermal Treatment.

    abstract::In this study, the changes in the global proteome of Salmonella in response to desiccation and thermal treatment were investigated by using an iTRAQ multiplex technique. A Salmonella enterica serovar Typhimurium strain was dried, equilibrated at high (1.0) and low (0.11) water activity (aw), and thermally treated at 7...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00393-18

    authors: Maserati A,Lourenco A,Diez-Gonzalez F,Fink RC

    更新日期:2018-08-31 00:00:00

  • Discrimination of psychrotrophic and mesophilic strains of the Bacillus cereus group by PCR targeting of major cold shock protein genes.

    abstract::Detection of psychrotrophic strains (those able to grow at or below 7 degreesC) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted stra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.9.3525-3529.1998

    authors: Francis KP,Mayr R,von Stetten F,Stewart GS,Scherer S

    更新日期:1998-09-01 00:00:00

  • Streptococcus cremoris M12R transconjugants carrying the conjugal plasmid pTR2030 are insensitive to attack by lytic bacteriophages.

    abstract::Conjugal transfer of lactose-fermenting ability (Lac+), nisin resistance (Nisr), and phage resistance (Hsp+) was demonstrated in matings between Streptococcus lactis ME2 (donor) and Streptococcus cremoris M43a (recipient), a derivative of M12R. Transconjugants were detected by transfer of Lac+ and were found to exhibi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.851-858.1985

    authors: Steenson LR,Klaenhammer TR

    更新日期:1985-10-01 00:00:00

  • Phenotypic diversity caused by differential RpoS activity among environmental Escherichia coli isolates.

    abstract::Enteric bacteria deposited into the environment by animal hosts are subject to diverse selective pressures. These pressures may act on phenotypic differences in bacterial populations and select adaptive mutations for survival in stress. As a model to study phenotypic diversity in environmental bacteria, we examined mu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05274-11

    authors: Chiang SM,Dong T,Edge TA,Schellhorn HE

    更新日期:2011-11-01 00:00:00

  • Visual evidence of horizontal gene transfer between plants and bacteria in the phytosphere of transplastomic tobacco.

    abstract::Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02632-08

    authors: Pontiroli A,Rizzi A,Simonet P,Daffonchio D,Vogel TM,Monier JM

    更新日期:2009-05-01 00:00:00

  • Agar overlay method to measure adherence of Staphylococcus epidermidis to four plastic surfaces.

    abstract::The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.5.1322-1324.1985

    authors: Mackenzie AM,Rivera-Calderon RL

    更新日期:1985-11-01 00:00:00

  • Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.

    abstract::A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4888-4892.2005

    authors: Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

    更新日期:2005-08-01 00:00:00

  • Sucrose synthesis in the nitrogen-fixing Cyanobacterium Anabaena sp. strain PCC 7120 is controlled by the two-component response regulator OrrA.

    abstract::The filamentous, nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 accumulates sucrose as a compatible solute against salt stress. Sucrose-phosphate synthase activity, which is responsible for the sucrose synthesis, is increased by salt stress, but the mechanism underlying the regulation of sucrose synthesis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01501-14

    authors: Ehira S,Kimura S,Miyazaki S,Ohmori M

    更新日期:2014-09-01 00:00:00

  • Detection of Vibrio cholerae O1 in the aquatic environment by fluorescent-monoclonal antibody and culture methods.

    abstract::Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.8.2370-2373.1990

    authors: Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

    更新日期:1990-08-01 00:00:00

  • Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk.

    abstract::Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.8.3177-3179.1995

    authors: Proctor ME,Brosch R,Mellen JW,Garrett LA,Kaspar CW,Luchansky JB

    更新日期:1995-08-01 00:00:00

  • The explosive-degrading cytochrome P450 system is highly conserved among strains of Rhodococcus spp.

    abstract::Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a widely used explosive and a serious environmental pollutant. Nineteen strains of Rhodococcus spp. capable of utilizing RDX as the sole nitrogen source have been isolated. The cytochrome P450 system XplA-XplB, which is responsible for RDX breakdown, is present in 18 of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00391-08

    authors: Seth-Smith HM,Edwards J,Rosser SJ,Rathbone DA,Bruce NC

    更新日期:2008-07-01 00:00:00

  • Occurrence of beta-Aminoglutaric Acid in Marine Bacteria.

    abstract::beta-Aminoglutaric acid, a nonprotein amino acid isomer of glutamic acid, was found in the free amino acid pool of a marine bacterium, Alteromonas luteoviolacea. It was also found in a mixed culture of fermenting bacteria enriched from an anoxic marine sediment. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.2.543-545.1985

    authors: Henrichs SM,Cuhel R

    更新日期:1985-08-01 00:00:00

  • Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestors and aerobic composters.

    abstract::A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.8.4618-4627.2003

    authors: Burtscher C,Wuertz S

    更新日期:2003-08-01 00:00:00

  • Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts.

    abstract::Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01196-07

    authors: Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

    更新日期:2007-10-01 00:00:00

  • Novel Bacillus thuringiensis insecticidal crystal protein with a silent activity against coleopteran larvae.

    abstract::A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.8.2536-2542.1992

    authors: Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

    更新日期:1992-08-01 00:00:00

  • Role of filamentous phage SW1 in regulating the lateral flagella of Shewanella piezotolerans strain WP3 at low temperatures.

    abstract::Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01675-13

    authors: Jian H,Xiao X,Wang F

    更新日期:2013-11-01 00:00:00