Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol.

abstract：:Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

journal_title：Applied and environmental microbiology

authors： Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

更新日期：1987-05-01 00:00:00

abstract：:A new method is described in which the Salkowski reaction is used for the rapid selection of alkaloid-producing mutants of the ergot fungus. This method was used to investigate the influence of a second mutation with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) on various mutants selected by a preliminary NTG mutation o...

journal_title：Applied and environmental microbiology

authors： Srikrai S,Robbers JE

更新日期：1983-04-01 00:00:00

abstract：:Thiolase (acetyl coenzyme A acetyltransferase; EC 2.3.1.9) from Clostridium acetobutylicum is a key enzyme in the production of acids and solvents in this organism. The purification and properties of the enzyme have already been described (D. P. Wiesenborn, F. B. Rudolph, and E.T. Papoutsakis, Appl. Environ. Microbiol...

journal_title：Applied and environmental microbiology

authors： Petersen DJ,Bennett GN

更新日期：1991-09-01 00:00:00

abstract：:A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains ...

journal_title：Applied and environmental microbiology

authors： Wozny MA,Bryant MP,Holdeman LV,Moore WE

更新日期：1977-05-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N(2) as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production, photooxygen evolution, and resp...

journal_title：Applied and environmental microbiology

authors： Kumazawa S,Mitsui A

更新日期：1985-08-01 00:00:00

abstract：:Biofilm-associated bacteria are less sensitive to antibiotics than free-living (planktonic) cells. Furthermore, with variations in the concentration of antibiotics throughout a biofilm, microbial cells are often exposed to levels below inhibitory concentrations and may develop resistance. This, as well as the irrespon...

journal_title：Applied and environmental microbiology

authors： Algburi A,Comito N,Kashtanov D,Dicks LMT,Chikindas ML

更新日期：2017-01-17 00:00:00

abstract：:Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance ...

journal_title：Applied and environmental microbiology

authors： Robey M,Benito A,Hutson RH,Pascual C,Park SF,Mackey BM

更新日期：2001-10-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit the application of t...

journal_title：Applied and environmental microbiology

authors： Suzuki MT,Taylor LT,DeLong EF

更新日期：2000-11-01 00:00:00

abstract：:Six strains of a new species, Legionella sainthelensi, were isolated from freshwater in areas affected by the volcanic eruptions of Mt. St. Helens in the state of Washington. Strains of L. sainthelensi are culturally and biochemically similar to other legionellae. They grow on buffered charcoal yeast agar but not on m...

journal_title：Applied and environmental microbiology

authors： Campbell J,Bibb WF,Lambert MA,Eng S,Steigerwalt AG,Allard J,Moss CW,Brenner DJ

更新日期：1984-02-01 00:00:00

abstract：:Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...

journal_title：Applied and environmental microbiology

authors： Miller AJ,Bayles DO,Eblen BS

更新日期：2000-10-01 00:00:00

abstract：:The bacterial communities associated with the development of necroses in injured agria cactus tissue were examined in the field by using both human-induced injuries and cactus tissue inoculated with cactophilic bacteria. Whole-cell bacterial fatty acids were used to determine when and where each of 23 detected species...

journal_title：Applied and environmental microbiology

authors： Foster JL,Fogleman JC

更新日期：1994-02-01 00:00:00

abstract：:Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

journal_title：Applied and environmental microbiology

authors： Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

更新日期：2012-06-01 00:00:00

abstract：:Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

journal_title：Applied and environmental microbiology

authors： Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

更新日期：2002-02-01 00:00:00

abstract：:The protein content of once-washed pellets of Dunaliella primalecta was 35 to 48%. Protein quality was good. Electron microscopy confirmed the absence of a cell wall. ...

journal_title：Applied and environmental microbiology

authors： Gibbs N,Duffus CM

更新日期：1976-04-01 00:00:00

abstract：:The maximum growth temperature, the optimal growth temperature, and the estimated normal physiological range for growth of Shewanella gelidimarina are functions of water activity (a(w)), which can be manipulated by changing the concentration of sodium chloride. The growth temperatures at the boundaries of the normal p...

journal_title：Applied and environmental microbiology

authors： Nichols DS,Olley J,Garda H,Brenner RR,McMeekin TA

更新日期：2000-06-01 00:00:00

abstract：:Duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. A panel of group-specific fluorescent in situ hybridization probes targeting 16S rRNA sequences revealed that the fermentors supported growth of a greater propo...

journal_title：Applied and environmental microbiology

authors： Duncan SH,Scott KP,Ramsay AG,Harmsen HJ,Welling GW,Stewart CS,Flint HJ

更新日期：2003-02-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...

journal_title：Applied and environmental microbiology

authors： Warth AD

更新日期：1988-08-01 00:00:00

abstract：:The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or 37 degrees C), pH (5.5 or 7.4), the presence of salt and organic acids (375 mM NaCl, 8.45 mM sodium diacetate [SD], 275 mM...

journal_title：Applied and environmental microbiology

authors： Garner MR,James KE,Callahan MC,Wiedmann M,Boor KJ

更新日期：2006-08-01 00:00:00

abstract：:Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

journal_title：Applied and environmental microbiology

authors： Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

更新日期：2007-01-01 00:00:00

abstract：:We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) usi...

journal_title：Applied and environmental microbiology

authors： Lee J,Jurgenson JE,Leslie JF,Bowden RL

更新日期：2008-04-01 00:00:00

abstract：:The persistence of hepatitis A virus (HAV) was determined both in mixtures of septic tank effluent (STE) with dairy cattle manure slurry (DCMS) and in mixtures of STE with swine manure slurry (SMS). HAV was consistently inactivated more rapidly in the two types of mixed wastes than in STE alone or in the control Dulbe...

journal_title：Applied and environmental microbiology

authors： Deng MY,Cliver DO

更新日期：1995-01-01 00:00:00

abstract：:A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon. ...

journal_title：Applied and environmental microbiology

authors： Bernasconi C,Volponi G,Picozzi C,Foschino R

更新日期：2007-10-01 00:00:00

abstract：:Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

journal_title：Applied and environmental microbiology

authors： Mallison SM 3rd,Cannon RE

更新日期：1984-05-01 00:00:00

abstract：:The fungus Cunninghamella elegans ATCC 36112 metabolized approximately 80% of the 3-14C-labeled fluoranthene (FA) added within 72 h of incubation. C. elegans metabolized FA to trans-2,3-dihydroxy-2,3-dihydrofluoranthene (trans-2,3-dihydrodiol), 8- and 9-hydroxyfluoranthene trans-2,3-dihydrodiol, 3-fluoranthene-beta-gl...

journal_title：Applied and environmental microbiology

authors： Pothuluri JV,Freeman JP,Evans FE,Cerniglia CE

更新日期：1990-10-01 00:00:00

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00