Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC 2.3.1.9) gene.

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:This study investigated the efficiency of methane and ammonium for stimulating trichloroethylene (TCE) biodegradation in groundwater microcosms (flasks and batch exchange columns) at a psychrophilic temperature (12 degrees C) typical of shallow aquifers in the northern United States or a mesophilic temperature (24 deg...

journal_title：Applied and environmental microbiology

authors： Moran BN,Hickey WJ

更新日期：1997-10-01 00:00:00

abstract：:Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

journal_title：Applied and environmental microbiology

authors： Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

更新日期：2007-05-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00

abstract：:During the course of immunologic studies involving the gastrointestinal colonization of mice with Candida albicans, it became apparent that the animals were being exposed to large numbers of Aspergillus fumigatus spores which interfered with the C. albicans colonization. To determine the source of the A. fumigatus exp...

journal_title：Applied and environmental microbiology

authors： Mayeux P,Dupepe L,Dunn K,Balsamo J,Domer J

更新日期：1995-06-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-...

journal_title：Applied and environmental microbiology

authors： Bretschger O,Obraztsova A,Sturm CA,Chang IS,Gorby YA,Reed SB,Culley DE,Reardon CL,Barua S,Romine MF,Zhou J,Beliaev AS,Bouhenni R,Saffarini D,Mansfeld F,Kim BH,Fredrickson JK,Nealson KH

更新日期：2007-11-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:The actions of cyclomaltodextrin glucanotransferases (CGTase; EC 2.4.1.19) from alkalophilic Bacillus sp. strain A2-5a (A2-5a CGTase), Bacillus macerans (Bmac CGTase), and Bacillus stearothermophilus (Bste CGTase) on amylose were investigated. All three enzymes produced large cyclic alpha-1,4-glucans (cycloamyloses) a...

journal_title：Applied and environmental microbiology

authors： Terada Y,Sanbe H,Takaha T,Kitahata S,Koizumi K,Okada S

更新日期：2001-04-01 00:00:00

abstract：:Actinomycete isolates from indoor air and dust in water-damaged schools and children's day care centers were tested for toxicity by using boar spermatozoa as an indicator. Toxicity was detected in extracts of four strains which caused a loss of sperm motility, and the 50% effective concentrations (EC50) were 10 to 63 ...

journal_title：Applied and environmental microbiology

authors： Andersson MA,Mikkola R,Kroppenstedt RM,Rainey FA,Peltola J,Helin J,Sivonen K,Salkinoja-Salonen MS

更新日期：1998-12-01 00:00:00

abstract：:Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

journal_title：Applied and environmental microbiology

authors： Halpern M,Gancz H,Broza M,Kashi Y

更新日期：2003-07-01 00:00:00

abstract：:[This corrects the article on p. 522 in vol. 52.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1987-01-01 00:00:00

abstract：:DNA from over 300 Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis isolates was analyzed by fluorescent amplified fragment length polymorphism (AFLP). B. thuringiensis and B. cereus isolates were from diverse sources and locations, including soil, clinical isolates and food products causing diarrheal an...

journal_title：Applied and environmental microbiology

authors： Hill KK,Ticknor LO,Okinaka RT,Asay M,Blair H,Bliss KA,Laker M,Pardington PE,Richardson AP,Tonks M,Beecher DJ,Kemp JD,Kolstø AB,Wong AC,Keim P,Jackson PJ

更新日期：2004-02-01 00:00:00

abstract：:Gram-positive Streptomyces bacteria produce thousands of bioactive secondary metabolites, including antibiotics. To systematically investigate genes affecting secondary metabolism, we developed a hyperactive transposase-based Tn5 transposition system and employed it to mutagenize the model species Streptomyces coelico...

journal_title：Applied and environmental microbiology

authors： Xu Z,Wang Y,Chater KF,Ou HY,Xu HH,Deng Z,Tao M

更新日期：2017-03-02 00:00:00

abstract：:The form of a previously developed Bĕlehrádek type of growth rate model was used to develop a probability model for defining the growth/no growth interface as a function of temperature (10 to 37 degrees C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM), and water activity (0.955 to 0.999; NaCl was used a...

journal_title：Applied and environmental microbiology

authors： Presser KA,Ross T,Ratkowsky DA

更新日期：1998-05-01 00:00:00

abstract：:An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition ...

journal_title：Applied and environmental microbiology

authors： Shin K,Oh I,Kim C

更新日期：1997-05-01 00:00:00

abstract：:The interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative (')alpha-zearalenol ((')alpha-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 microg ml(-1)) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A consider...

journal_title：Applied and environmental microbiology

authors： El-Nezami H,Polychronaki N,Salminen S,Mykkänen H

更新日期：2002-07-01 00:00:00

abstract：:In the presence of added sulfate, 2,4-dichlorophenol and 4-chlorophenol were transformed stoichiometrically to 4-chlorophenol and phenol, respectively, in anaerobic freshwater lake sediments between 18 and 40 degrees C. The concomitantly occurring sulfate reduction reduced the initial sulfate concentration from 25 mM ...

journal_title：Applied and environmental microbiology

authors： Kohring GW,Zhang XM,Wiegel J

更新日期：1989-10-01 00:00:00

abstract：:The rumen anaerobic fungus Neocallimastix frontalis was grown on cellulosic substrates, and the cellular distribution and types of glycosidases produced by the organism were studied. Fungal cultures were fractionated into extracellular, insoluble (membrane), and intracellular fractions and assayed for glycosidase acti...

journal_title：Applied and environmental microbiology

authors： Pearce PD,Bauchop T

更新日期：1985-05-01 00:00:00

abstract：:Fenton reactions are believed to play important roles in wood degradation by brown rot fungi. In this context, the effect of tropolone (2-hydroxycyclohepta-2,4,6-trienone), a metal chelator, on wood degradation by Poria placenta was investigated. Tropolone (50 micro M) strongly inhibits fungal growth on malt agar, but...

journal_title：Applied and environmental microbiology

authors： Diouf PN,Delbarre N,Perrin D,Gérardin P,Rapin C,Jacquot JP,Gelhaye E

更新日期：2002-09-01 00:00:00

abstract：:Laboratory-grown strains of chemoautotrophic Thiomicrospira sp. strain L-12 and Thiobacillus neapolitanus produced cell carbon that was 24.6 to 25.1 ppt (24.6 to 25.1 mg/g) lower in C isotope abundance than the ambient source of carbon dioxide and bicarbonate. This degree of C isotope depletion was comparable to that ...

journal_title：Applied and environmental microbiology

authors： Ruby EG,Jannasch HW,Deuser WG

更新日期：1987-08-01 00:00:00

abstract：:Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontaminat...

journal_title：Applied and environmental microbiology

authors： Schmithausen RM,Kellner SR,Schulze-Geisthoevel SV,Hack S,Engelhart S,Bodenstein I,Al-Sabti N,Reif M,Fimmers R,Körber-Irrgang B,Harlizius J,Hoerauf A,Exner M,Bierbaum G,Petersen B,Bekeredjian-Ding I

更新日期：2015-11-01 00:00:00

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

journal_title：Applied and environmental microbiology

authors： Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

更新日期：2008-04-01 00:00:00

abstract：:Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detectable amounts of CH...

journal_title：Applied and environmental microbiology

authors： Harper DB,Buswell JA,Kennedy JT,Hamilton JT

更新日期：1990-11-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

journal_title：Applied and environmental microbiology

authors： Macalady JL,Mack EE,Nelson DC,Scow KM

更新日期：2000-04-01 00:00:00

abstract：:The growth response of Acacia mangium Willd. to inoculation with selected Bradyrhizobium strains was investigated in two field trials in the Ivory Coast (West Africa). In the first trial (Anguededou), four provenances (i.e., trees originating from seeds harvested in different geographical areas) of A. mangium were ino...

journal_title：Applied and environmental microbiology

authors： Galiana A,Prin Y,Mallet B,Gnahoua GM,Poitel M,Diem HG

更新日期：1994-11-01 00:00:00

abstract：:A beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalGlyNAcP) homolog gene was cloned from Vibrio vulnificus CMCP6. In synthetic reactions, the recombinant enzyme acted only with GlcNAc and GalNAc as acceptors in the presence of alpha-d-galactose-1-phosphate as a donor to form lacto-N-biose I (LNB) (Galbeta1 -->...

journal_title：Applied and environmental microbiology

authors： Nakajima M,Kitaoka M

更新日期：2008-10-01 00:00:00