Use of phospholipid fatty acids to detect previous self-heating events in stored peat.

abstract：:A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation t...

journal_title：Applied and environmental microbiology

authors： Jung SC,Smith CL,Lee KS,Hong ME,Kweon DH,Stephanopoulos G,Jin YS

更新日期：2010-09-01 00:00:00

abstract：:Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen ...

journal_title：Applied and environmental microbiology

authors： Gibbons RJ,Dankers I

更新日期：1981-04-01 00:00:00

abstract：:Methyl fluoride is frequently used to specifically inhibit acetoclastic methanogenesis, thus allowing determination of the relative contribution of acetate versus H2/CO2 to total CH4 production in natural environments. However, the effect of the inhibitor on growth of the target archaeal population has not yet been st...

journal_title：Applied and environmental microbiology

authors： Penning H,Conrad R

更新日期：2006-01-01 00:00:00

abstract：:Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

journal_title：Applied and environmental microbiology

authors： Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

更新日期：1993-06-01 00:00:00

abstract：UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...

journal_title：Applied and environmental microbiology

authors： Peterz M,Butot S,Jagadeesan B,Bakker D,Donaghy J

更新日期：2016-04-18 00:00:00

abstract：:Population sizes of algae, bacteria, heterotrophic flagellates, and viruses were observed through the 1989 spring diatom bloom in Raunefjorden in western Norway. The culmination of the diatom bloom was followed by a peak in the concentration of bacteria and an increase in the concentration of heterotrophic flagellates...

journal_title：Applied and environmental microbiology

authors： Bratbak G,Heldal M,Norland S,Thingstad TF

更新日期：1990-05-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 microM). De...

journal_title：Applied and environmental microbiology

authors： Massieux B,Boivin ME,Van Den Ende FP,Langenskiöld J,Marvan P,Barranguet C,Admiraal W,Laanbroek HJ,Zwart G

更新日期：2004-08-01 00:00:00

abstract：:Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem stro...

journal_title：Applied and environmental microbiology

authors： Xiaoqiang W,Vennison SJ,Huirong L,Ben-Dov E,Zaritsky A,Boussiba S

更新日期：1997-12-01 00:00:00

abstract：:Hexamita sp. is an amitochondriate free-living diplomonad which inhabits O(2)-limited environments, such as the deep waters and sediments of lakes and marine basins. C nuclear magnetic resonance spectroscopy reveals ethanol, lactate, acetate, and alanine as products of glucose fermentation under microaerobic condition...

journal_title：Applied and environmental microbiology

authors： Biagini GA,McIntyre PS,Finlay BJ,Lloyd D

更新日期：1998-01-01 00:00:00

abstract：:The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remain...

journal_title：Applied and environmental microbiology

authors： Highfield AC,El Nagar A,Mackinder LC,Noël LM,Hall MJ,Martin SJ,Schroeder DC

更新日期：2009-11-01 00:00:00

abstract：:Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests th...

journal_title：Applied and environmental microbiology

authors： Zozaya-Hinchliffe M,Martin DH,Ferris MJ

更新日期：2008-03-01 00:00:00

abstract：:The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

journal_title：Applied and environmental microbiology

authors： Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

更新日期：1993-05-01 00:00:00

abstract：:Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

journal_title：Applied and environmental microbiology

authors： Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

更新日期：2003-06-01 00:00:00

abstract：:A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and is...

journal_title：Applied and environmental microbiology

authors： Brauer KL,Robbers JE

更新日期：1987-01-01 00:00:00

abstract：:The following two factors significantly influence estimates of the maximum specific growth rate ( micro (max)) and the lag-phase duration (lambda): (i) the technique used to monitor bacterial growth and (ii) the model fitted to estimate parameters. In this study, nine strains of Listeria monocytogenes were monitored s...

journal_title：Applied and environmental microbiology

authors： Baty F,Flandrois JP,Delignette-Muller ML

更新日期：2002-12-01 00:00:00

abstract：:The anaerobic bacterium Eubacterium lentum, a common constituent of the intestinal microflora, inactivates digoxin by reducing the unsaturated lactone ring. Reduction of the cardiac glycoside by growing cultures of E. lentum ATCC 25559 proceeded in a stereospecific manner, with the 20R-dihydrodigoxin constituting more...

journal_title：Applied and environmental microbiology

authors： Robertson LW,Chandrasekaran A,Reuning RH,Hui J,Rawal BD

更新日期：1986-06-01 00:00:00

abstract：:Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isot...

journal_title：Applied and environmental microbiology

authors： Sun W,Sun X,Cupples AM

更新日期：2012-04-01 00:00:00

abstract：:Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

journal_title：Applied and environmental microbiology

authors： Ziołecki A,Wojciechowicz M

更新日期：1980-04-01 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabo...

journal_title：Applied and environmental microbiology

authors： Boopathy R

更新日期：1996-09-01 00:00:00

abstract：:Compost amendment and inoculations with specific microorganisms are fundamentally different soil treatment methods, commonly used in agriculture for the improvement of plant growth and health. Although distinct, both methods affect the rhizosphere and the plant roots. In the present study we used a 16S rRNA gene appro...

journal_title：Applied and environmental microbiology

authors： Ofek M,Hadar Y,Minz D

更新日期：2009-10-01 00:00:00

abstract：:Under anoxic conditions, most methoxylated mononuclear aromatic compounds are degraded by bacteria, with catechol being formed as an important intermediate. On the basis of our experiments with the sulfate-reducing bacterium Desulfobacterium sp. strain Cat2, we describe for the first time the enzymatic activities invo...

journal_title：Applied and environmental microbiology

authors： Gorny N,Schink B

更新日期：1994-09-01 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:Thirty-three standard mycotoxins were assayed by thin-layer chromatography and by cytotoxicity in HEp-2 and Chang cells. Various levels of detection were found. The cytotoxicity test was significantly more sensitive than thin-layer chromatography for the trichothecenes and should be useful for screening extracts from ...

journal_title：Applied and environmental microbiology

authors： Robb J,Norval M

更新日期：1983-10-01 00:00:00

abstract：:We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Johnson SB,Vrijenhoek RC

更新日期：2007-04-01 00:00:00

abstract：:In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...

journal_title：Applied and environmental microbiology

authors： Xiao Z,Bergeron H,Grosse S,Beauchemin M,Garron ML,Shaya D,Sulea T,Cygler M,Lau PC

更新日期：2008-02-01 00:00:00

abstract：:1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli express...

journal_title：Applied and environmental microbiology

authors： Altaras NE,Cameron DC

更新日期：1999-03-01 00:00:00

abstract：:Oligodeoxynucleotide hybridization probes were developed to complement specific regions of the small subunit (SSU) rRNA sequences of cultivated and uncultivated cyanobacteria and Chloroflexus-like bacteria, which inhabit hot spring microbial mats. The probes were used to investigate the natural distribution of SSU rRN...

journal_title：Applied and environmental microbiology

authors： Ruff-Roberts AL,Kuenen JG,Ward DM

更新日期：1994-02-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00