Distribution of cultivated and uncultivated cyanobacteria and Chloroflexus-like bacteria in hot spring microbial mats.

abstract：:A new thermostable dipeptidase gene was cloned from the thermophile Brevibacillus borstelensis BCS-1 by genetic complementation of the D-Glu auxotroph Escherichia coli WM335 on a plate containing D-Ala-D-Glu. Nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid...

journal_title：Applied and environmental microbiology

authors： Baek DH,Song JJ,Kwon SJ,Park C,Jung CM,Sung MH

更新日期：2004-03-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:The genus Bradyrhizobium has been considered to be a taxonomically difficult group. In this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among Bradyrhizobium strains nodulating soybeans in China. Eleven genospecies were identified by sequence analysis of three phyl...

journal_title：Applied and environmental microbiology

authors： Zhang XX,Guo HJ,Wang R,Sui XH,Zhang YM,Wang ET,Tian CF,Chen WX

更新日期：2014-05-01 00:00:00

abstract：:Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the ac...

journal_title：Applied and environmental microbiology

authors： Yoshida N,Takahashi N,Hiraishi A

更新日期：2005-08-01 00:00:00

abstract：:[This corrects the article on p. 711 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-11-01 00:00:00

abstract：:By targeted deletion of the polyglutamate operon (pga) in Bacillus licheniformis F11, a derivative form, F11.1 (Δpga), was obtained that, along with lacking polyglutamate (PGA) formation, displayed enhanced proteolytic activities. The phenotypic properties were maintained in a strain in which the chiBA operon was addi...

journal_title：Applied and environmental microbiology

authors： Hoffmann K,Daum G,Köster M,Kulicke WM,Meyer-Rammes H,Bisping B,Meinhardt F

更新日期：2010-12-01 00:00:00

abstract：:Anaerobic ammonium oxidation is a recent addition to the microbial nitrogen cycle, and its metabolic pathway, including the production and conversion of its intermediate hydrazine, is not well understood. Therefore, the effect of hydroxylamine addition on the hydrazine metabolism of anaerobic ammonium-oxidizing (anamm...

journal_title：Applied and environmental microbiology

authors： van der Star WR,van de Graaf MJ,Kartal B,Picioreanu C,Jetten MS,van Loosdrecht MC

更新日期：2008-07-01 00:00:00

abstract：:The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 ...

journal_title：Applied and environmental microbiology

authors： Thorgersen MP,Lancaster WA,Vaccaro BJ,Poole FL,Rocha AM,Mehlhorn T,Pettenato A,Ray J,Waters RJ,Melnyk RA,Chakraborty R,Hazen TC,Deutschbauer AM,Arkin AP,Adams MW

更新日期：2015-08-01 00:00:00

abstract：:Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

journal_title：Applied and environmental microbiology

authors： Szabó I,Benedek A,Barabás G

更新日期：1985-08-01 00:00:00

abstract：:Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed throughout strata that included fine-grained lacustrine (lake) sediments, a paleosol (buried soil) sequence, and coarse-grai...

journal_title：Applied and environmental microbiology

authors： Kieft TL,Fredrickson JK,McKinley JP,Bjornstad BN,Rawson SA,Phelps TJ,Brockman FJ,Pfiffner SM

更新日期：1995-02-01 00:00:00

abstract：:The expression of xenobiotic-degradative genes in indigenous bacteria or in bacteria introduced into an ecosystem is essential for the successful bioremediation of contaminated environments. The maintenance of naphthalene utilization activity is studied in Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. (strain...

journal_title：Applied and environmental microbiology

authors： Guerin WF,Boyd SA

更新日期：1995-11-01 00:00:00

abstract：:We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an ...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Lewis DA,Ocampo-Sosa AA,Fogarty U,Makrai L,Navas J,Scortti M,Hernández M,Vázquez-Boland JA

更新日期：2006-06-01 00:00:00

abstract：:The existence of anaerobic biodegradation of lignin was examined in mixed microflora. Egyptian soil samples, in which rapid mineralization of organic matter takes place in the presence of an important anaerobic microflora, were used to obtain the anaerobic enrichment cultures for this study. Specifically, 14CO2 or [14...

journal_title：Applied and environmental microbiology

authors： Odier E,Monties B

更新日期：1983-09-01 00:00:00

abstract：:We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of ap...

journal_title：Applied and environmental microbiology

authors： Stepkowski T,Moulin L,Krzyzańska A,McInnes A,Law IJ,Howieson J

更新日期：2005-11-01 00:00:00

abstract：:Conditioned medium was obtained from suspension cultures of soybean (Glycine max L. Merrit) cells after incubating them for 4 to 8 days with rhizobia which were separated from the soybean cells by two dialysis bags, one within another. This conditioned medium from the plant cell side (PCM) of the two membranes was use...

journal_title：Applied and environmental microbiology

authors： Bednarski MA,Reporter M

更新日期：1978-07-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

journal_title：Applied and environmental microbiology

authors： Scholz BK,Jakobek JL,Lindgren PB

更新日期：1994-04-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:Identifying and overcoming the limitations preventing efficient high-yield production of chemicals remain important tasks in metabolic engineering. In an attempt to rewire Corynebacterium glutamicum to produce ethanol, we attained a low yield (63% of the theoretical) when using resting cells on glucose, and large amou...

journal_title：Applied and environmental microbiology

authors： Shen J,Chen J,Solem C,Jensen PR,Liu JM

更新日期：2020-11-24 00:00:00

abstract：:Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of th...

journal_title：Applied and environmental microbiology

authors： Fassel TA,Buchholz LA,Collins ML,Remsen CC

更新日期：1992-07-01 00:00:00

abstract：:Vibrio fischeri colonizes the squid Euprymna scolopes in a mutualistic symbiosis. Hatchling squid lack these bacterial symbionts, and V. fischeri strains must compete to occupy this privileged niche. We cloned a V. fischeri gene, designated pilA, that contributes to colonization competitiveness and encodes a protein s...

journal_title：Applied and environmental microbiology

authors： Stabb EV,Ruby EG

更新日期：2003-02-01 00:00:00

abstract：:Seven Azospirillum strains induced more deformation of root hairs of wheat than did strains of Rhizobium leguminosarum, Azotobacter chroococcum, or Escherichia coli. Azospirillum sp. strain Sp245 caused the most deformation. Strain Sp245 (isolated from surface sterile roots of wheat) and strain Sp7 (isolated from the ...

journal_title：Applied and environmental microbiology

authors： Jain DK,Patriquin DG

更新日期：1984-12-01 00:00:00

abstract：:The objective of this study was to determine the effect of high pressure (HP) on the inactivation of microbial contaminants in Cheddar cheese (Escherichia coli K-12, Staphylococcus aureus ATCC 6538, and Penicillium roqueforti IMI 297987). Initially, cheese slurries inoculated with E. coli, S. aureus, and P. roqueforti...

journal_title：Applied and environmental microbiology

authors： O'Reilly CE,O'Connor PM,Kelly AL,Beresford TP,Murphy PM

更新日期：2000-11-01 00:00:00

abstract：:The exopolysaccharides (EPSs) produced by some bacteria are potential growth substrates for other bacteria in soil. We used stable-isotope probing (SIP) to identify aerobic soil bacteria that assimilated the cellulose produced by Gluconacetobacter xylinus or the EPS produced by Beijerinckia indica. The latter is a het...

journal_title：Applied and environmental microbiology

authors： Wang X,Sharp CE,Jones GM,Grasby SE,Brady AL,Dunfield PF

更新日期：2015-07-01 00:00:00

abstract：:The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

journal_title：Applied and environmental microbiology

authors： Phan HC,Wade SA,Blackall LL

更新日期：2020-06-17 00:00:00

abstract：:Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

journal_title：Applied and environmental microbiology

authors： McGowan C,Fulthorpe R,Wright A,Tiedje JM

更新日期：1998-10-01 00:00:00

abstract：:A metabolic pathway for the catabolism of propionate is proposed. This pathway incorporates a transcarboxylation reaction involving propionyl coenzyme A and oxaloacetate and a carboxylation of pyruvate to regenerate oxaloacetate. Data indicated that the proposed pathway is reversible. The proposed pathway and its appa...

journal_title：Applied and environmental microbiology

authors： Robbins JE

更新日期：1988-05-01 00:00:00

abstract：:Data from analyses of continuous culture fermentation of insoluble cellulose by Ruminococcus albus 7 were used to derive constants for the rate of cellulose hydrolysis and fermentation, growth yield, and maintenance. Cellulose concentration was 1% in the nutrient reservoir, and hydraulic retention times of 0.5, 1.0, 1...

journal_title：Applied and environmental microbiology

authors： Pavlostathis SG,Miller TL,Wolin MJ

更新日期：1988-11-01 00:00:00

abstract：:Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...

journal_title：Applied and environmental microbiology

authors： Woegerbauer M,Jenni B,Thalhammer F,Graninger W,Burgmann H

更新日期：2002-01-01 00:00:00

abstract：:Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

journal_title：Applied and environmental microbiology

authors： Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

更新日期：2018-06-18 00:00:00

abstract：:A colorimetric assay for NADPH-dependent, mercuric ion-specific oxidoreductase activity was developed to facilitate the investigation of mercuric reductase gene expression in polluted aquatic ecosystems. Protein molecules extracted directly from unseeded freshwater and samples seeded with Pseudomonas aeruginosa PU21 (...

journal_title：Applied and environmental microbiology

authors： Ogunseitan OA

更新日期：1998-02-01 00:00:00