Improvement of the thermostability and activity of a pectate lyase by single amino acid substitutions, using a strategy based on melting-temperature-guided sequence alignment.

abstract：:Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, wh...

journal_title：Applied and environmental microbiology

authors： Coletta-Filho HD,Takita MA,de Souza AA,Aguilar-Vildoso CI,Machado MA

更新日期：2001-09-01 00:00:00

abstract：:In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes. ...

journal_title：Applied and environmental microbiology

authors： Cao M,Bitar AP,Marquis H

更新日期：2007-04-01 00:00:00

abstract：:Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...

journal_title：Applied and environmental microbiology

authors： Kästner M,Breuer-Jammali M,Mahro B

更新日期：1998-01-01 00:00:00

abstract：:In Europe, ixodid ticks are important arthropod vectors of human and animal pathogens, but comprehensive studies of the prevalence of all relevant pathogens in Central Europe are scarce. As a result of ecological changes, the incidences of tick-borne infections are expected to increase. In this study, 1,394 nymphal an...

journal_title：Applied and environmental microbiology

authors： Reye AL,Hübschen JM,Sausy A,Muller CP

更新日期：2010-05-01 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

journal_title：Applied and environmental microbiology

authors： Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

更新日期：2017-02-15 00:00:00

abstract：:A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

journal_title：Applied and environmental microbiology

authors： Gorski TW,Ritzert RW

更新日期：1977-08-01 00:00:00

abstract：:Bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute to the defense of the host plant against colonization of fouling organisms. However, the number of bacterial cells necessary to defend against fouling on the plant surface is not known. Pseudoalteromonas tunicata and Ph...

journal_title：Applied and environmental microbiology

authors： Rao D,Webb JS,Holmström C,Case R,Low A,Steinberg P,Kjelleberg S

更新日期：2007-12-01 00:00:00

abstract：UNLABELLED:Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in a healthy population. We conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of Bifidobacterium bifidum strain Bb on the IME of adult hea...

journal_title：Applied and environmental microbiology

authors： Gargari G,Taverniti V,Balzaretti S,Ferrario C,Gardana C,Simonetti P,Guglielmetti S

更新日期：2016-09-16 00:00:00

abstract：:[This corrects the article on p. 483 in vol. 37.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1979-09-01 00:00:00

abstract：:The protein content of once-washed pellets of Dunaliella primalecta was 35 to 48%. Protein quality was good. Electron microscopy confirmed the absence of a cell wall. ...

journal_title：Applied and environmental microbiology

authors： Gibbs N,Duffus CM

更新日期：1976-04-01 00:00:00

abstract：:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

journal_title：Applied and environmental microbiology

authors： Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

更新日期：1998-10-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

journal_title：Applied and environmental microbiology

authors： Wells JM,Cole RJ,Cutler HC,Spalding DH

更新日期：1981-04-01 00:00:00

abstract：:Aerobic activated sludge granules are dense, spherical biofilms which can strongly improve purification efficiency and sludge settling in wastewater treatment processes. In this study, the structure and development of different granule types were analyzed. Biofilm samples originated from lab-scale sequencing batch rea...

journal_title：Applied and environmental microbiology

authors： Weber SD,Ludwig W,Schleifer KH,Fried J

更新日期：2007-10-01 00:00:00

abstract：:Monitoring cell growth and measuring physical features of food-borne pathogenic bacteria are important for better understanding the conditions under which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead Escherichia coli O157:H7 and Listeria innocua were measured usin...

journal_title：Applied and environmental microbiology

authors： Lewis CL,Craig CC,Senecal AG

更新日期：2014-06-01 00:00:00

abstract：:In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, wh...

journal_title：Applied and environmental microbiology

authors： Cocolin L,Urso R,Rantsiou K,Cantoni C,Comi G

更新日期：2006-01-01 00:00:00

abstract：:A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was ...

journal_title：Applied and environmental microbiology

authors： Biavati B,Vasta M,Ferry JG

更新日期：1988-03-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:The 3-phenoxybenzoate (3-PBA) 1',2'-dioxygenase gene cluster (pbaA1A2B cluster), which is responsible for catalyzing 3-phenoxybenzoate to 3-hydroxybenzoate and catechol, is inducibly expressed in Sphingobium wenxiniae strain JZ-1(T) by its substrate 3-PBA. In this study, we identified a transcriptional activator of th...

journal_title：Applied and environmental microbiology

authors： Cheng M,Chen K,Guo S,Huang X,He J,Li S,Jiang J

更新日期：2015-12-01 00:00:00

abstract：:The effect of the glutathione reductase (GshR) activity of Lactobacillus sanfranciscensis DSM20451(T) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. The gshR gene coding for a putative GshR was sequenced and inactivated by single-crossover integr...

journal_title：Applied and environmental microbiology

authors： Jänsch A,Korakli M,Vogel RF,Gänzle MG

更新日期：2007-07-01 00:00:00

abstract：:Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

journal_title：Applied and environmental microbiology

authors： Tompkin RB,Christiansen LN,Shaparis AB

更新日期：1978-01-01 00:00:00

abstract：:Iron(III)-reducing bacteria have been demonstrated to rapidly catalyze the reduction and immobilization of uranium(VI) from contaminated subsurface sediments. Thus, these organisms may aid in the development of bioremediation strategies for uranium contamination, which is prevalent in acidic subsurface sediments at U....

journal_title：Applied and environmental microbiology

authors： Petrie L,North NN,Dollhopf SL,Balkwill DL,Kostka JE

更新日期：2003-12-01 00:00:00

abstract：:Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely in nutrient-rich, fresh, and brackish waters, and its toxic blooms cause the death of domestic animals. The administration of a crude toxic cell extract of M. aeruginosa K-139 to mice can produce tumor necrosis factor (TNF) and prompt...

journal_title：Applied and environmental microbiology

authors： Nakano Y,Shirai M,Mori N,Nakano M

更新日期：1991-01-01 00:00:00

abstract：:In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the...

journal_title：Applied and environmental microbiology

authors： Pélandakis M,De Jonckheere JF,Pernin P

更新日期：1998-08-01 00:00:00

abstract：:O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Te(r)), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were con...

journal_title：Applied and environmental microbiology

authors： Yin X,Wheatcroft R,Chambers JR,Liu B,Zhu J,Gyles CL

更新日期：2009-09-01 00:00:00

abstract：:A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

journal_title：Applied and environmental microbiology

authors： Kennedy MJ,Lawless JG

更新日期：1985-01-01 00:00:00

abstract：:Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

journal_title：Applied and environmental microbiology

authors： Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

更新日期：2018-08-17 00:00:00

abstract：:Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabo...

journal_title：Applied and environmental microbiology

authors： Morrow KM,Bromhall K,Motti CA,Munn CB,Bourne DG

更新日期：2016-12-15 00:00:00

abstract：:Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

journal_title：Applied and environmental microbiology

authors： Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

更新日期：1986-03-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00