Abstract:
:Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Xiaoqiang W,Vennison SJ,Huirong L,Ben-Dov E,Zaritsky A,Boussiba Sdoi
10.1128/AEM.63.12.4971-4974.1997subject
Has Abstractpub_date
1997-12-01 00:00:00pages
4971-4issue
12eissn
0099-2240issn
1098-5336journal_volume
63pub_type
杂志文章abstract::Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are l-rhamnose and d-glucose. C. beijerinckii DSM 6423 ut...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02656-18
更新日期:2019-02-20 00:00:00
abstract::Air drying of raw sludge caused inactivation of indigenous viruses. A gradual loss of infectivity occurred with the loss of water until the solids content reached about 80%. A more rapid decline of viral infectivity occurred with further dewatering. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.6.1943-1945.1983
更新日期:1983-06-01 00:00:00
abstract::A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was al...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07501-11
更新日期:2012-04-01 00:00:00
abstract::Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.12.3868-3872.1992
更新日期:1992-12-01 00:00:00
abstract::Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.9.4457-4464.2002
更新日期:2002-09-01 00:00:00
abstract::The biogeography of the purple nonsulfur bacterium Rhodopseudomonas palustris on a local scale was investigated. Thirty clones of phototrophic bacteria were isolated from each of five unevenly spaced sampling locations in freshwater marsh sediments along a linear 10-m transect, and a total of 150 clones were character...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.9.5186-5191.2003
更新日期:2003-09-01 00:00:00
abstract::Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.2.492-503.1981
更新日期:1981-02-01 00:00:00
abstract::Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.6.2206-2212.1997
更新日期:1997-06-01 00:00:00
abstract::In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02166-10
更新日期:2011-03-01 00:00:00
abstract::The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4440-4447.2001
更新日期:2001-10-01 00:00:00
abstract::Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01336-19
更新日期:2019-10-16 00:00:00
abstract::A microscopically pure enrichment culture of a gram-negative anaerobic bacterium, in the present article referred to as PER-K23, was isolated from an anaerobic packed-bed column in which tetrachloroethene (PCE) was reductively transformed to ethane via trichloroethene (TCE), cis-1,2-dichloroethene (cis-1,2-DCE), chlor...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.9.2991-2997.1993
更新日期:1993-09-01 00:00:00
abstract::Although the biological conversion of nitrite to nitrate is a well-known process, studies of Nitrobacter populations are hindered by their physiological characteristics. This report describes a new method for detecting and counting Nitrobacter populations in situ with the PCR. Two primers from the 16S rRNA gene were u...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.6.2093-2098.1995
更新日期:1995-06-01 00:00:00
abstract::Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.4.1913-1922.2004
更新日期:2004-04-01 00:00:00
abstract::UV light only penetrates liquid food surfaces to a very short depth, thereby limiting its industrial application in food pasteurization. One promising alternative is the combination of UV light with mild heat (UV-H), which has been demonstrated to produce a synergistic bactericidal effect. The aim of this article is t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00623-13
更新日期:2013-07-01 00:00:00
abstract::The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00624-08
更新日期:2008-11-01 00:00:00
abstract::Freshwater, sewage, and fecal samples from various sources were examined for Rhodococcus coprophilus, associated actinomycetes, Escherichia coli, and fecal streptococci. Rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.6.1037-1042.1981
更新日期:1981-12-01 00:00:00
abstract::A computer program was developed to identify anaerobic bacteria by using simultaneous pattern recognition via a Bayesian probabilistic model. The system is intended for use as a rapid, precise, and reproducible aid in the identification of unknown isolates. The program operates on a data base of 28 genera comprising 2...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.3.507-511.1978
更新日期:1978-03-01 00:00:00
abstract::Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purif...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01362-10
更新日期:2010-11-01 00:00:00
abstract::Prototrophic and growth factor-requiring strains of Alcaligenes spp. were used to study the effect of a protozoan, Tetrahymena pyriformis, on the degradation of p-aminobenzoate. The protozoan inhibited activity of the prototrophic bacterium by reducing its population size. For the growth factor-requiring strain of Alc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.1.229-232.1981
更新日期:1981-01-01 00:00:00
abstract::In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.7.2331-2337.1996
更新日期:1996-07-01 00:00:00
abstract::The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01529-07
更新日期:2008-02-01 00:00:00
abstract::Presently, the only effective treatment for celiac disease is a life-long gluten-free diet. In this work, we used a new mixture of selected sourdough lactobacilli and fungal proteases to eliminate the toxicity of wheat flour during long-time fermentation. Immunological (R5 antibody-based sandwich and competitive enzym...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00260-07
更新日期:2007-07-01 00:00:00
abstract::The Pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. Their diversity influences the phylogenetic diversity and heterogeneity of these communities. On the basis of average amino acid iden...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02612-15
更新日期:2015-10-30 00:00:00
abstract::Plasmodium falciparum, in addition to scavenging essential fatty acids from its intra- and intercellular environments, possesses a functional complement of type II fatty acid synthase (FAS) enzymes targeted to the apicoplast organelle. Recent evidence suggests that products of the plasmodial FAS II system may be criti...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00170-10
更新日期:2010-06-01 00:00:00
abstract::The microbial degradation of tensile test pieces made of poly(3-hydroxybutyrate) [P(3HB)] or a copolymer of 90% 3-hydroxybutyric acid and 10% 3-hydroxyvaleric acid was studied in soils incubated at a constant temperature of 15, 28, or 40 degrees C for up to 200 days. In addition, hydrolytic degradation in sterile buff...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.10.3233-3238.1993
更新日期:1993-10-01 00:00:00
abstract::The formation of DNA photoproducts in organisms exposed to ambient levels of UV-B radiation can lead to death and/or reduced population growth in aquatic systems. Dependence on photoenzymatic repair to reverse DNA damage caused by UV-B radiation is demonstrated for Paraphysomonas sp., a member of a widely distributed ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02803-08
更新日期:2009-07-01 00:00:00
abstract::The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.7.3314-3318.2001
更新日期:2001-07-01 00:00:00
abstract:UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00710-16
更新日期:2016-06-30 00:00:00
abstract::Escherichia coli offers unparalleled engineering capacity in the context of heterologous natural product biosynthesis. However, as with other heterologous hosts, cellular metabolism must be designed or redesigned to support final compound formation. This task is at once complicated and aided by the fact that the cell ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
doi:10.1128/AEM.07391-11
更新日期:2012-04-01 00:00:00