Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis.

Abstract:

:Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria.

journal_name

Appl Environ Microbiol

authors

Xiaoqiang W,Vennison SJ,Huirong L,Ben-Dov E,Zaritsky A,Boussiba S

doi

10.1128/AEM.63.12.4971-4974.1997

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

4971-4

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

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