Monoclonal antibodies that detect live salmonellae.

Abstract:

:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.

journal_name

Appl Environ Microbiol

authors

Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

doi

10.1128/AEM.58.12.3868-3872.1992

keywords:

subject

Has Abstract

pub_date

1992-12-01 00:00:00

pages

3868-72

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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