Use of the Escherichia coli beta-Glucuronidase (gusA) Gene as a Reporter Gene for Analyzing Promoters in Lactic Acid Bacteria.

abstract：:The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to c...

journal_title：Applied and environmental microbiology

authors： Clarkson SM,Giannone RJ,Kridelbaugh DM,Elkins JG,Guss AM,Michener JK

更新日期：2017-08-31 00:00:00

abstract：:The role of light as an energy source for slightly aereated cultures of halobacteria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non-illuminated and differently illuminated cultures and with different oxygen transfer rat...

journal_title：Applied and environmental microbiology

authors： Rodriguez-Valera F,Nieto JJ,Ruiz-Berraquero F

更新日期：1983-03-01 00:00:00

abstract：:Ribosomal gene sequences were obtained from bryozoans in the genus Bugula and their bacterial symbionts; analyses of host and symbiont phylogenetic trees did not support a history of strict cospeciation. Symbiont-derived compounds known to defend host larvae from predation were only detected in two out of four symbiot...

journal_title：Applied and environmental microbiology

authors： Lim-Fong GE,Regali LA,Haygood MG

更新日期：2008-06-01 00:00:00

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00

abstract：:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as lon...

journal_title：Applied and environmental microbiology

authors： Whitby GE,Murray RG

更新日期：1980-01-01 00:00:00

abstract：:Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...

journal_title：Applied and environmental microbiology

authors： Asgeirsdóttir SA,Scholtmeijer K,Wessels JG

更新日期：1999-05-01 00:00:00

abstract：:Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

journal_title：Applied and environmental microbiology

authors： Macalady JL,Mack EE,Nelson DC,Scow KM

更新日期：2000-04-01 00:00:00

abstract：:Seventeen Bradyrhizobium sp. strains and one Azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16S rRNA gene (rDNA), (ii) randomly amplified DNA polymorphisms (RAPD) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv)...

journal_title：Applied and environmental microbiology

authors： Van Rossum D,Schuurmans FP,Gillis M,Muyotcha A,Van Verseveld HW,Stouthamer AH,Boogerd FC

更新日期：1995-04-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:The development of high-throughput methods, such as the construction of 18S rRNA gene clone or pyrosequencing libraries, has allowed evaluation of ciliate community composition in hundreds of samples from the rumen and other intestinal habitats. However, several genera of mammalian intestinal ciliates have been descri...

journal_title：Applied and environmental microbiology

authors： Kittelmann S,Devente SR,Kirk MR,Seedorf H,Dehority BA,Janssen PH

更新日期：2015-04-01 00:00:00

abstract：:Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

journal_title：Applied and environmental microbiology

authors： Buhr TL,Dickman MB

更新日期：1997-06-01 00:00:00

abstract：:Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carr...

journal_title：Applied and environmental microbiology

authors： Deleo PC,Baveye P

更新日期：1996-12-01 00:00:00

abstract：:A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

journal_title：Applied and environmental microbiology

authors： Lee C,Langlois BE,Dawson KA

更新日期：1993-05-01 00:00:00

abstract：:Corynebacterium glutamicum is able to grow in media containing up to 12 mM arsenite and 500 mM arsenate and is one of the most arsenic-resistant microorganisms described to date. Two operons (ars1 and ars2) involved in arsenate and arsenite resistance have been identified in the complete genome sequence of Corynebacte...

journal_title：Applied and environmental microbiology

authors： Ordóñez E,Letek M,Valbuena N,Gil JA,Mateos LM

更新日期：2005-10-01 00:00:00

abstract：:Experimental infection of animals with aerosols containing pathogenic agents is essential for an understanding of the natural history and pathogenesis of infectious disease as well as evaluation of potential treatments. We evaluated whether the Aeroneb nebulizer, a vibrating mesh nebulizer, would serve as an alternati...

journal_title：Applied and environmental microbiology

authors： Bowling JD,O'Malley KJ,Klimstra WB,Hartman AL,Reed DS

更新日期：2019-08-14 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed...

journal_title：Applied and environmental microbiology

authors： Faure D,Bouillant ML,Bally R

更新日期：1994-09-01 00:00:00

abstract：:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to...

journal_title：Applied and environmental microbiology

authors： Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff ME

更新日期：2007-10-01 00:00:00

abstract：:Metabolic engineering and multisubunit protein production necessitate the expression of multiple genes at coordinated levels. In bacteria, genes for multisubunit proteins or metabolic pathways are often expressed in operons under the control of a single promoter; expression of the genes is coordinated by varying trans...

journal_title：Applied and environmental microbiology

authors： Smolke CD,Carrier TA,Keasling JD

更新日期：2000-12-01 00:00:00

abstract：:Veratryl alcohol, added as a supplement to cultures of Phanerochaete chrysosporium, enhanced ligninase activity through protection of the ligninase against inactivation by hydrogen peroxide produced by this fungus in cultures. In the presence of veratryl alcohol, the loss of ligninase activity observed in non-protein-...

journal_title：Applied and environmental microbiology

authors： Tonon F,Odier E

更新日期：1988-02-01 00:00:00

abstract：:Piriformospora indica (Hymenomycetes, Basidiomycota) is a newly described cultivable endophyte that colonizes roots. Inoculation with the fungus and application of fungal culture filtrate promotes plant growth and biomass production. Due to its ease of culture, this fungus provides a model organism for the study of be...

journal_title：Applied and environmental microbiology

authors： Varma A,Savita Verma,Sudha,Sahay N,Butehorn B,Franken P

更新日期：1999-06-01 00:00:00

abstract：:The pH and the nature an concentration of simple electrolytes influenced the interaction of poliovirus type 2 with three soils, a sand, and a clay mineral. In electrolytes above pH 9 the virus was not adsorbed extensively to the substrates, but below pH 7 almost all virus was bound. For each adsorbent there was a char...

journal_title：Applied and environmental microbiology

authors： Taylor DH,Moore RS,Sturman LS

更新日期：1981-12-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:The ability of the facultative photoheterotroph Rhodobacter sphaeroides to tolerate and reduce high levels of tellurite in addition to at least 10 other rare earth metal oxides and oxyanions has considerable potential for detoxification and bioremediation of contaminated environments. We report the identification and ...

journal_title：Applied and environmental microbiology

authors： O'Gara JP,Gomelsky M,Kaplan S

更新日期：1997-12-01 00:00:00

abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried i...

journal_title：Applied and environmental microbiology

authors： Van Horn DJ,Okie JG,Buelow HN,Gooseff MN,Barrett JE,Takacs-Vesbach CD

更新日期：2014-05-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...

journal_title：Applied and environmental microbiology

authors： Johnson CN,Bowers JC,Griffitt KJ,Molina V,Clostio RW,Pei S,Laws E,Paranjpye RN,Strom MS,Chen A,Hasan NA,Huq A,Noriea NF 3rd,Grimes DJ,Colwell RR

更新日期：2012-10-01 00:00:00

abstract：:A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was al...

journal_title：Applied and environmental microbiology

authors： Heylen K,Ettwig K,Hu Z,Jetten M,Kartal B

更新日期：2012-04-01 00:00:00