Abstract:
:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to cause a robust polyclonal immunoglobulin G (IgG) response when used to immunize rabbits. Antibodies against these recombinant proteins bound to the native forms of each protein as harvested from in vitro grown biofilms of MRSA, as determined both via Western blot analysis and immunofluorescence confocal microscopy. These IgGs could be utilized as imaging tools that localize to areas of specific protein production within a biofilm. This work illustrates that immunogenic, cell wall-associated, biofilm-upregulated proteins are promising for in vitro visualization of biofilm growth, architecture, and space-function relationships.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff MEdoi
10.1128/AEM.00855-07subject
Has Abstractpub_date
2007-10-01 00:00:00pages
6612-9issue
20eissn
0099-2240issn
1098-5336pii
AEM.00855-07journal_volume
73pub_type
杂志文章abstract::Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by c...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00354-11
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pub_type: 杂志文章
doi:10.1128/AEM.62.8.2783-2788.1996
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.5.994-1000.1984
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doi:10.1128/AEM.36.2.213-216.1978
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.31.6.949-958.1976
更新日期:1976-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.55.2.520-522.1989
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pub_type: 杂志文章
doi:10.1128/AEM.59.7.2286-2292.1993
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doi:10.1128/AEM.72.3.2185-2190.2006
更新日期:2006-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.3.694-700.1991
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pub_type: 杂志文章
doi:10.1128/AEM.34.1.80-87.1977
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.8.1901-1906.1989
更新日期:1989-08-01 00:00:00
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doi:10.1128/AEM.39.3.635-641.1980
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doi:10.1128/AEM.59.2.628-630.1993
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.4.539-543.1978
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.3.633-641.1979
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abstract::The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW expo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.5.1206-1208.1986
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.5.1105-1111.1977
更新日期:1977-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.64.9.3202-3208.1998
更新日期:1998-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00322-20
更新日期:2020-06-17 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.2.555-560.1988
更新日期:1988-02-01 00:00:00
abstract::Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.9.5613-5620.2004
更新日期:2004-09-01 00:00:00