Real-time PCR analysis of the intestinal microbiotas in peritoneal dialysis patients.

abstract：:"Fingerprints" of strains of methanol-oxidizing bacteria were obtained by exposing them to a set of chemicals which could stimulate or inhibit the growth. The chemicals gave quantitative results, which were used to calculate the similarities between the strains. The method has been used for establishing identity or no...

journal_title：Applied and environmental microbiology

authors： Kühn I

更新日期：1978-11-01 00:00:00

abstract：:A push-pull method, previously used in groundwater analyses, was successfully adapted for measuring sulfide turnover rates in situ at different depths in the meromictic Lake Cadagno. In the layer of phototrophic bacteria at about 12 m in depth net sulfide consumption was observed during the day, indicating active bact...

journal_title：Applied and environmental microbiology

authors： Lüthy L,Fritz M,Bachofen R

更新日期：2000-02-01 00:00:00

abstract：:A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forwar...

journal_title：Applied and environmental microbiology

authors： Reilly K,Attwood GT

更新日期：1998-03-01 00:00:00

abstract：:The sensitivity of two recently isolated antigenic variants of echovirus type 25 (Montpellier 76-1262 and Thionville 86-222) to glutaraldehyde (GTA) at low concentrations was compared with that of the JV-4 prototype strain. The purified viruses were treated under the same conditions with GTA at concentrations ranging ...

journal_title：Applied and environmental microbiology

authors： Chambon M,Bailly JL,Peigue-Lafeuille H

更新日期：1994-02-01 00:00:00

abstract：:Two different Cd(2+) uptake systems were identified in Lactobacillus plantarum. One is a high-affinity, high-velocity Mn(2+) uptake system which also takes up Cd(2+) and is induced by Mn(2+) starvation. The calculated K(m) and V(max) are 0.26 microM and 3.6 micromol g of dry cell(-1) min(-1), respectively. Unlike Mn(2...

journal_title：Applied and environmental microbiology

authors： Hao Z,Reiske HR,Wilson DB

更新日期：1999-11-01 00:00:00

abstract：:Salinity effects on microbial community structure and on potential rates of arsenate reduction, arsenite oxidation, sulfate reduction, denitrification, and methanogenesis were examined in sediment slurries from two California soda lakes. We conducted experiments with Mono Lake and Searles Lake sediments over a wide ra...

journal_title：Applied and environmental microbiology

authors： Kulp TR,Han S,Saltikov CW,Lanoil BD,Zargar K,Oremland RS

更新日期：2007-08-01 00:00:00

abstract：:A variant of Bacillus thuringiensis subsp. kurstaki containing a single, stable copy of a uniquely amplifiable DNA oligomer integrated into the genome for tracking the fate of biological agents in the environment was developed. The use of genetically tagged spores overcomes the ambiguity of discerning the test materia...

journal_title：Applied and environmental microbiology

authors： Emanuel PA,Buckley PE,Sutton TA,Edmonds JM,Bailey AM,Rivers BA,Kim MH,Ginley WJ,Keiser CC,Doherty RW,Kragl FJ,Narayanan FE,Katoski SE,Paikoff S,Leppert SP,Strawbridge JB,VanReenen DR,Biberos SS,Moore D,Phillips DW,

更新日期：2012-12-01 00:00:00

abstract：UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...

journal_title：Applied and environmental microbiology

authors： Wang S,Doona CJ,Setlow P,Li YQ

更新日期：2016-09-16 00:00:00

abstract：:Six monoclonal antibodies directed against enterobacteria were produced and characterized. The specificity of one of these antibodies (CX9/15; immunoglobulin G2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. All of the enterobacteria were specific...

journal_title：Applied and environmental microbiology

authors： Levasseur S,Husson MO,Leitz R,Merlin F,Laurent F,Peladan F,Drocourt JL,Leclerc H,Van Hoegaerden M

更新日期：1992-05-01 00:00:00

abstract：:Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

journal_title：Applied and environmental microbiology

authors： Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

更新日期：2015-09-01 00:00:00

abstract：:The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid o...

journal_title：Applied and environmental microbiology

authors： Koyama Y,Okamoto S,Furukawa K

更新日期：1990-07-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:Biobleaching of hardwood unbleached kraft pulp (UKP) by Phanerochaete chrysosporium and Trametes versicolor was studied in the solid-state fermentation system with different culture media. In this fermentation system with low-nitrogen and high-carbon culture medium, pulp brightness increased by 15 and 30 points after ...

journal_title：Applied and environmental microbiology

authors： Katagiri N,Tsutsumi Y,Nishida T

更新日期：1995-02-01 00:00:00

abstract：:Several wild-type isolates of marine bdellovibrios formed stable bdelloplasts when they infected gram-negative bacterial prey under certain culture conditions. Synchronous predator-prey cultures and low nutrient concentrations increased the yield of stable bdelloplasts. The bdellovibrio cells retained in the stable bd...

journal_title：Applied and environmental microbiology

authors： Sánchez-Amat A,Torrella F

更新日期：1990-09-01 00:00:00

abstract：:Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...

journal_title：Applied and environmental microbiology

authors： Leathers TD

更新日期：1986-11-01 00:00:00

abstract：:Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

journal_title：Applied and environmental microbiology

authors： Chrisope GL,Fox CW,Marshall RT

更新日期：1976-05-01 00:00:00

abstract：:Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

journal_title：Applied and environmental microbiology

authors： Hewson I,Fuhrman JA

更新日期：2004-06-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U.S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin ge...

journal_title：Applied and environmental microbiology

authors： Xia X,Meng J,McDermott PF,Ayers S,Blickenstaff K,Tran TT,Abbott J,Zheng J,Zhao S

更新日期：2010-03-01 00:00:00

abstract：:The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

journal_title：Applied and environmental microbiology

authors： Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

更新日期：1996-06-01 00:00:00

abstract：:We investigated whether the yield of the B vitamin folic acid could be elevated in Bacillus subtilis. Strategies for increasing the folic acid yield were investigated by employing computer-aided flux analysis and mutation. Controlling the activity of the enzyme pyruvate kinase by placing it under inducible control was...

journal_title：Applied and environmental microbiology

authors： Zhu T,Pan Z,Domagalski N,Koepsel R,Ataai MM,Domach MM

更新日期：2005-11-01 00:00:00

abstract：:To longitudinally assess fecal shedding and animal-to-animal transmission of Clostridium difficile among finishing feedlot cattle as a risk for beef carcass contamination, we tested 186 ± 12 steers (mean ± standard deviation; 1,369 samples) in an experimental feedlot facility during the finishing period and at harvest...

journal_title：Applied and environmental microbiology

authors： Rodriguez-Palacios A,Pickworth C,Loerch S,LeJeune JT

更新日期：2011-05-01 00:00:00

abstract：:The purpose of this study was to examine host distribution patterns among fecal bacteria in the order Bacteroidales, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. We analyzed Bacteroidales 16S rRNA gene sequences from the feces of eight hosts: human, bovin...

journal_title：Applied and environmental microbiology

authors： Dick LK,Bernhard AE,Brodeur TJ,Santo Domingo JW,Simpson JM,Walters SP,Field KG

更新日期：2005-06-01 00:00:00

abstract：:We examined physiological adaptations which allow the psychrotroph Rhodococcus sp. strain Q15 to assimilate alkanes at a low temperature (alkanes are contaminants which are generally insoluble and/or solid at low temperatures). During growth at 5 degrees C on hexadecane or diesel fuel, strain Q15 produced a cell surfa...

journal_title：Applied and environmental microbiology

authors： Whyte LG,Slagman SJ,Pietrantonio F,Bourbonnière L,Koval SF,Lawrence JR,Inniss WE,Greer CW

更新日期：1999-07-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...

journal_title：Applied and environmental microbiology

authors： Robinson IM,Whipp SC,Bucklin JA,Allison MJ

更新日期：1984-11-01 00:00:00

abstract：:Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

journal_title：Applied and environmental microbiology

authors： Tompkin RB,Christiansen LN,Shaparis AB

更新日期：1978-01-01 00:00:00

abstract：:A Comamonas testosteroni strain was isolated from activated sludge on the basis of its ability to coaggregate with yeast cells. On agar plates the following two types of colonies were formed: colonies with a mucoid appearance and colonies with a nonmucoid appearance. On plates this strain alternated between the two fo...

journal_title：Applied and environmental microbiology

authors： Bossier P,Verstraete W

更新日期：1996-08-01 00:00:00

abstract：:This paper explores the relationship between the genetic diversity of rhizobia and the morphological diversity of their plant hosts. Rhizobium galegae strains were isolated from nodules of wild Galega orientalis and Galega officinalis in the Caucasus, the center of origin for G. orientalis. All 101 isolates were chara...

journal_title：Applied and environmental microbiology

authors： Andronov EE,Terefework Z,Roumiantseva ML,Dzyubenko NI,Onichtchouk OP,Kurchak ON,Dresler-Nurmi A,Young JP,Simarov BV,Lindström K

更新日期：2003-02-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00