Statistical approaches for estimating actinobacterial diversity in marine sediments.

abstract：:Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...

journal_title：Applied and environmental microbiology

authors： Luque-Almagro VM,Huertas MJ,Sáez LP,Luque-Romero MM,Moreno-Vivián C,Castillo F,Roldán MD,Blasco R

更新日期：2008-10-01 00:00:00

abstract：:Agromonas oligotrophica (Bradyrhizobium oligotrophicum) S58(T) is a nitrogen-fixing oligotrophic bacterium isolated from paddy field soil that is able to grow in extra-low-nutrient environments. Here, the complete genome sequence of S58 was determined. The S58 genome was found to comprise a circular chromosome of 8,26...

journal_title：Applied and environmental microbiology

authors： Okubo T,Fukushima S,Itakura M,Oshima K,Longtonglang A,Teaumroong N,Mitsui H,Hattori M,Hattori R,Hattori T,Minamisawa K

更新日期：2013-04-01 00:00:00

abstract：:Salivaricin A (SalA), the first Streptococcus salivarius lantibiotic to be characterized, appears to be inhibitory to most Streptococcus pyogenes strains. A variant of the SalA structural gene (salA1) is present in more than 90% of S. pyogenes strains, but only strains of M serotype 4 and T pattern 4 produce the biolo...

journal_title：Applied and environmental microbiology

authors： Wescombe PA,Upton M,Dierksen KP,Ragland NL,Sivabalan S,Wirawan RE,Inglis MA,Moore CJ,Walker GV,Chilcott CN,Jenkinson HF,Tagg JR

更新日期：2006-02-01 00:00:00

abstract：:The S85 type strain of Fibrobacter succinogenes, a major ruminal fibrolytic species, was isolated 49 years ago from a bovine rumen and has been used since then as a model for extensive studies. To assess the validity of this model, we compared the cellulase- and xylanase-degrading activities of several other F. succin...

journal_title：Applied and environmental microbiology

authors： Béra-Maillet C,Ribot Y,Forano E

更新日期：2004-04-01 00:00:00

abstract：:The maximum growth temperature, the optimal growth temperature, and the estimated normal physiological range for growth of Shewanella gelidimarina are functions of water activity (a(w)), which can be manipulated by changing the concentration of sodium chloride. The growth temperatures at the boundaries of the normal p...

journal_title：Applied and environmental microbiology

authors： Nichols DS,Olley J,Garda H,Brenner RR,McMeekin TA

更新日期：2000-06-01 00:00:00

abstract：:We examined the influence of elevated CO2 concentration on denitrifier enzyme activity in wheat rhizoplanes by using controlled environments and solution culture techniques. Potential denitrification activity was from 3 to 24 times higher on roots that were grown under an elevated CO2 concentration of 1,000 micromoles...

journal_title：Applied and environmental microbiology

authors： Smart DR,Ritchie K,Stark JM,Bugbee B

更新日期：1997-11-01 00:00:00

abstract：:Previous studies have demonstrated that Staphylococcus epidermidis isolates colonizing the skin of healthy humans do not typically encode icaADBC, the genes responsible for the production of polysaccharide intercellular adhesin or biofilms. It was therefore hypothesized that the presence of icaADBC was deleterious to ...

journal_title：Applied and environmental microbiology

authors： Rogers KL,Rupp ME,Fey PD

更新日期：2008-10-01 00:00:00

abstract：:Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

journal_title：Applied and environmental microbiology

authors： Kimura H,Sugihara M,Kato K,Hanada S

更新日期：2006-01-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

journal_title：Applied and environmental microbiology

authors： Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

更新日期：2013-04-01 00:00:00

abstract：:Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

journal_title：Applied and environmental microbiology

authors： Brown JC,Huang G,Haley-Zitlin V,Jiang X

更新日期：2009-02-01 00:00:00

abstract：:3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...

journal_title：Applied and environmental microbiology

authors： Zhang JY,Zou J,Bao Q,Chen WL,Wang L,Yang H,Zhang CC

更新日期：2006-01-01 00:00:00

abstract：:High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...

journal_title：Applied and environmental microbiology

authors： Beffa T,Blanc M,Lyon PF,Vogt G,Marchiani M,Fischer JL,Aragno M

更新日期：1996-05-01 00:00:00

abstract：:Despite the importance of human noroviruses (NoVs) in public health, little information concerning the effectiveness of ozone against NoVs is available. We determined the efficacy of ozone disinfection using murine norovirus (MNV) as a surrogate of human NoV. MNV in ozone demand-free buffer was exposed to a predetermi...

journal_title：Applied and environmental microbiology

authors： Lim MY,Kim JM,Lee JE,Ko G

更新日期：2010-02-01 00:00:00

abstract：:Different amounts of enterotoxin A-, B-, and C1-producing staphylococci were added to dry sausage prepared by normal processes, either alone or in conjunction with a starter culture (micrococci and lactobacilli). The sausage was examined after 0, 3, 7, 14, and 30 days for staphylococci, micrococci, and lactobacilli, a...

journal_title：Applied and environmental microbiology

authors： Niskanen A,Nurmi E

更新日期：1976-01-01 00:00:00

abstract：:The acetylene block technique was used to assay denitrification in undisturbed sediment cores of an intertidal mud flat. Nitrogen loss due to this process was estimated at 1 to 41 kg of N/hectare (ha) per year. Anaerobic nitrate-saturated slurry of the same sediment had a denitrification capacity of 1,026 kg of N/ha p...

journal_title：Applied and environmental microbiology

authors： Kaspar HF

更新日期：1982-03-01 00:00:00

abstract：:Growth, survival, and pathogenicity of Klebsiella growing in and on environmental foci were examined. Total coliforms present in raw wastes from pulp mills were in excess of 10(5)/ml, and 60 to 80% were Klebsiella. Fecal coliform counts ranged from 10(1) to 10(5)/ml. Klebsiella isolates from industrial effluents and a...

journal_title：Applied and environmental microbiology

authors： Knittel MD,Seidler RJ,Eby C,Cabe LM

更新日期：1977-11-01 00:00:00

abstract：:The undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of Listeria spp. In some cases, these microbial consortia inhibit Listeria almost completely. From such undefined industrial cheese-ripening floras, ...

journal_title：Applied and environmental microbiology

authors： Eppert I,Valdés-Stauber N,Götz H,Busse M,Scherer S

更新日期：1997-12-01 00:00:00

abstract：:The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...

journal_title：Applied and environmental microbiology

authors： Widmer G,Lee Y

更新日期：2010-10-01 00:00:00

abstract：:The current project was developed to examine inactivation of biofilm bacteria and to characterize the interaction of biocides with pipe surfaces. Unattached bacteria were quite susceptible to the variety of disinfectants tested. Viable bacterial counts were reduced 99% by exposure to 0.08 mg of hypochlorous acid (pH 7...

journal_title：Applied and environmental microbiology

authors： LeChevallier MW,Cawthon CD,Lee RG

更新日期：1988-10-01 00:00:00

abstract：:In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50...

journal_title：Applied and environmental microbiology

authors： Tan Y,Miller KJ

更新日期：1992-04-01 00:00:00

abstract：:Microbial transformation of the antimelanoma agent betulinic acid was studied. The main objective of this study was to utilize microorganisms as in vitro models to predict and prepare potential mammalian metabolites of this compound. Preparative-scale biotransformation with resting-cell suspensions of Bacillus megater...

journal_title：Applied and environmental microbiology

authors： Chatterjee P,Kouzi SA,Pezzuto JM,Hamann MT

更新日期：2000-09-01 00:00:00

abstract：:NisI confers immunity against nisin, with high substrate specificity to prevent a suicidal effect in nisin-producing Lactococcus lactis strains. However, the NisI maturation process as well as its influence on nisin resistance has not been characterized. Here, we report the roles of lipoprotein signal peptidase II (Ls...

journal_title：Applied and environmental microbiology

authors： Liu J,Xiong H,Du Y,Wiwatanaratanabutr I,Wu X,Zhao G,Zhu H,Caiyin Q,Qiao J

更新日期：2020-09-17 00:00:00

abstract：:Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. (sup13)C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and to probe citrate or pyruvate utilization, noninvasively, in living cell suspensions. The utilization of isotopical...

journal_title：Applied and environmental microbiology

authors： Ramos A,Lolkema JS,Konings WN,Santos H

更新日期：1995-04-01 00:00:00

abstract：:Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated...

journal_title：Applied and environmental microbiology

authors： Paddock CD,Fournier PE,Sumner JW,Goddard J,Elshenawy Y,Metcalfe MG,Loftis AD,Varela-Stokes A

更新日期：2010-05-01 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:Three common food pathogenic microorganisms were exposed to treatments simulating those used in food processing. Treated cell suspensions were then analyzed for reduction in growth by plate counting. Flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) were carried out on treated cells stained for membr...

journal_title：Applied and environmental microbiology

authors： Kennedy D,Cronin UP,Wilkinson MG

更新日期：2011-07-01 00:00:00

abstract：:A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

journal_title：Applied and environmental microbiology

authors： Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

更新日期：1986-09-01 00:00:00

abstract：:By using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the Aspergillus ochraceus group. A correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to...

journal_title：Applied and environmental microbiology

authors： Robbers JE,Hong S,Tuite J,Carlton WW

更新日期：1978-12-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00