Comparison of single- and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis.

abstract：:Mold-damaged building materials may contain biologically active agents, such as (1-->3)-beta-D-glucan, allergens, and mycotoxins, which have been associated with adverse health effects. The release of these components from contaminated surfaces into the air is not well understood. The purpose of this study was to char...

journal_title：Applied and environmental microbiology

authors： Seo SC,Reponen T,Levin L,Borchelt T,Grinshpun SA

更新日期：2008-02-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...

journal_title：Applied and environmental microbiology

authors： Chesson A,Stewart CS,Wallace RJ

更新日期：1982-09-01 00:00:00

abstract：:The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled wi...

journal_title：Applied and environmental microbiology

authors： Mullane NR,Ryan M,Iversen C,Murphy M,O'Gaora P,Quinn T,Whyte P,Wall PG,Fanning S

更新日期：2008-02-01 00:00:00

abstract：:Primers were designed to target 16S rRNA and nodD genes of Rhizobium leguminosarum from DNA extracted from two different soil types contaminated with Zn applied in sewage sludge. Numbers of rhizobia estimated using 16S rRNA gene copy number showed higher abundance than those estimated by both nodD and the most-probabl...

journal_title：Applied and environmental microbiology

authors： Macdonald CA,Clark IM,Hirsch PR,Zhao FJ,McGrath SP

更新日期：2011-07-01 00:00:00

abstract：:A rhizosphere Gram-positive bacterial isolate, Paenibacillus sp. NAIST15-1, exhibits intriguing motility behavior on hard agar medium. Paenibacillus sp. shows increased transcription of flagellar genes and hyperflagellation when transferred from liquid to solid medium. Hyperflagellated cells form wandering colonies th...

journal_title：Applied and environmental microbiology

authors： Kobayashi K,Kanesaki Y,Yoshikawa H

更新日期：2017-07-17 00:00:00

abstract：:Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...

journal_title：Applied and environmental microbiology

authors： Auman AJ,Stolyar S,Costello AM,Lidstrom ME

更新日期：2000-12-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

journal_title：Applied and environmental microbiology

authors： Abrahamsen LH,Jerkofsky M

更新日期：1981-03-01 00:00:00

abstract：:Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...

journal_title：Applied and environmental microbiology

authors： Amner W,McCarthy AJ,Edwards C

更新日期：1988-12-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

journal_title：Applied and environmental microbiology

authors： Atlas RM,Williams JF,Huntington MK

更新日期：1996-04-01 00:00:00

abstract：:A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for me...

journal_title：Applied and environmental microbiology

authors： Gallardo O,Fernández-Fernández M,Valls C,Valenzuela SV,Roncero MB,Vidal T,Díaz P,Pastor FI

更新日期：2010-09-01 00:00:00

abstract：:Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...

journal_title：Applied and environmental microbiology

authors： Tempelaars MH,Rodrigues S,Abee T

更新日期：2011-04-01 00:00:00

abstract：:Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

journal_title：Applied and environmental microbiology

authors： Cluss RG,Johnson JK,Somerson NL

更新日期：1983-08-01 00:00:00

abstract：:A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatogra...

journal_title：Applied and environmental microbiology

authors： Gendloff EH,Pestka JJ,Swanson SP,Hart LP

更新日期：1984-05-01 00:00:00

abstract：:The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabo...

journal_title：Applied and environmental microbiology

authors： Boopathy R

更新日期：1996-09-01 00:00:00

abstract：:Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and ...

journal_title：Applied and environmental microbiology

authors： Meunier A,Schmit JF,Stas A,Kutluk N,Bragard C

更新日期：2003-04-01 00:00:00

abstract：:Auxotrophic microorganisms are often used for genetic engineering, because their biosynthetic deficiency can be complemented by the transforming DNA and allows selection for transformants that have become prototrophic. However, when complementation is obtained by ectopic expression this may lead to unpredictable side ...

journal_title：Applied and environmental microbiology

authors： Nielsen ML,de Jongh WA,Meijer SL,Nielsen J,Mortensen UH

更新日期：2007-11-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00

abstract：:Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, "Natronoanaerobium," and Clostridium. Two novel therm...

journal_title：Applied and environmental microbiology

authors： Kaksonen AH,Plumb JJ,Robertson WJ,Spring S,Schumann P,Franzmann PD,Puhakka JA

更新日期：2006-05-01 00:00:00

abstract：:DNA of 10 lines of rice yellow dwarf (RYD) mycoplasmalike organisms (MLOs) from Japan, the Phillippines, and Thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal DNA of a Tochigi (Japan) line of RYD MLO. One chromosomal probe (RYD9) and all six extrachromosomal probes ...

journal_title：Applied and environmental microbiology

authors： Nakashima K,Kato S,Iwanami S,Murata N

更新日期：1993-04-01 00:00:00

abstract：:Enumeration of lactic acid streptococci from dairy starter cultures on Elliker agar was improved by the addition of 0.4% (wt/vol) diammonium phosphate. Agar plate counts were up to 7.75 times greater due to improved buffering by the phosphate salt. ...

journal_title：Applied and environmental microbiology

authors： Barach JT

更新日期：1979-07-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:The size frequency distribution of planktonic cells of purple sulfur phototrophic bacteria was measured at several depths in a bacterial layer of Lake Cisó (Spain). The bacterioplankton was dominated by Chromatium minus (87 to 94% of the total biomass). The largest cells of C. minus were found in the top part of the b...

journal_title：Applied and environmental microbiology

authors： Montesinos E

更新日期：1987-04-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:To overcome the limited availability of antibiotic resistance markers in filamentous fungi, we adapted the FLP/FRT recombination system from the yeast Saccharomyces cerevisiae for marker recycling. We tested this system in the penicillin producer Penicillium chrysogenum using different experimental approaches. In a tw...

journal_title：Applied and environmental microbiology

authors： Kopke K,Hoff B,Kück U

更新日期：2010-07-01 00:00:00