Liposomes replace serum for cultivation of fermenting mycoplasmas.

abstract：:Most probable numbers (MPNs) of methanogens in various salt marsh and estuarine sediments were determined with an anaerobic, habitat-simulating culture medium with 80% H(2) plus 20% CO(2) as substrate. Average MPNs for the short Spartina (SS) marsh sediments of Sapelo Island, Ga., were maximal at the 5- to 7-cm depth ...

journal_title：Applied and environmental microbiology

authors： Jones WJ,Paynter MJ

更新日期：1980-04-01 00:00:00

abstract：:The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliab...

journal_title：Applied and environmental microbiology

authors： Jørgensen TR,Nitsche BM,Lamers GE,Arentshorst M,van den Hondel CA,Ram AF

更新日期：2010-08-01 00:00:00

abstract：:The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

journal_title：Applied and environmental microbiology

authors： Marciano-Cabral FM,Fulford DE

更新日期：1986-05-01 00:00:00

abstract：:The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid o...

journal_title：Applied and environmental microbiology

authors： Koyama Y,Okamoto S,Furukawa K

更新日期：1990-07-01 00:00:00

abstract：:The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme acti...

journal_title：Applied and environmental microbiology

authors： Daniel G,Volc J,Kubatova E

更新日期：1994-07-01 00:00:00

abstract：:Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

journal_title：Applied and environmental microbiology

authors： Geng M,Smith L

更新日期：2018-07-17 00:00:00

abstract：:Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

journal_title：Applied and environmental microbiology

authors： Davey HM

更新日期：2011-08-15 00:00:00

abstract：:Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here, we describe chains of interconnecting vesicles protruding from cells of strain Hel3_A1_48, affiliating with Formosa spp. within the Flavo...

journal_title：Applied and environmental microbiology

authors： Fischer T,Schorb M,Reintjes G,Kolovou A,Santarella-Mellwig R,Markert S,Rhiel E,Littmann S,Becher D,Schweder T,Harder J

更新日期：2019-09-17 00:00:00

abstract：:Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...

journal_title：Applied and environmental microbiology

authors： Knudsen GM,Ng Y,Gram L

更新日期：2013-12-01 00:00:00

abstract：:Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

journal_title：Applied and environmental microbiology

authors： Chrisope GL,Fox CW,Marshall RT

更新日期：1976-05-01 00:00:00

abstract：:Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the ...

journal_title：Applied and environmental microbiology

authors： Armstrong JL,Calomiris JJ,Seidler RJ

更新日期：1982-08-01 00:00:00

abstract：:The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in com...

journal_title：Applied and environmental microbiology

authors： Chourey K,Thompson MR,Morrell-Falvey J,Verberkmoes NC,Brown SD,Shah M,Zhou J,Doktycz M,Hettich RL,Thompson DK

更新日期：2006-09-01 00:00:00

abstract：:Synthesis of the ligninolytic system of the wood-degrading fungus Phanerochaete chrysosporium is induced during secondary metabolism, brought about by nitrogen, carbon, or sulfur starvation. We describe here a strategy for selection of mutants which are ligninolytic (lignin----CO2) and overproduce lignin-degrading enz...

journal_title：Applied and environmental microbiology

authors： Tien M,Myer SB

更新日期：1990-08-01 00:00:00

abstract：:Thirty-three standard mycotoxins were assayed by thin-layer chromatography and by cytotoxicity in HEp-2 and Chang cells. Various levels of detection were found. The cytotoxicity test was significantly more sensitive than thin-layer chromatography for the trichothecenes and should be useful for screening extracts from ...

journal_title：Applied and environmental microbiology

authors： Robb J,Norval M

更新日期：1983-10-01 00:00:00

abstract：:The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryp...

journal_title：Applied and environmental microbiology

authors： Marston CK,Hoffmaster AR,Wilson KE,Bragg SL,Plikaytis B,Brachman P,Johnson S,Kaufmann AF,Popovic T

更新日期：2005-12-01 00:00:00

abstract：:Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typi...

journal_title：Applied and environmental microbiology

authors： Varshney AK,Mediavilla JR,Robiou N,Guh A,Wang X,Gialanella P,Levi MH,Kreiswirth BN,Fries BC

更新日期：2009-11-01 00:00:00

abstract：:Chickens on a broiler farm in southern England were found to be colonized with Campylobacter jejuni of a single serotype, Lior 1 Penner 4. The farm was the sole supplier of a local slaughterhouse associated with a campylobacter outbreak in 1984 caused by this serotype. The serotype persisted on the farm for at least 1...

journal_title：Applied and environmental microbiology

authors： Pearson AD,Greenwood M,Healing TD,Rollins D,Shahamat M,Donaldson J,Colwell RR

更新日期：1993-04-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...

journal_title：Applied and environmental microbiology

authors： Cafaro V,Notomista E,Capasso P,Di Donato A

更新日期：2005-08-01 00:00:00

abstract：:SAR11 bacteria are small, heterotrophic, marine alphaproteobacteria found throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean conditions, although the adaptations required for life under those conditions are not well understood. To illuminate this issue, we used cryo-electron to...

journal_title：Applied and environmental microbiology

authors： Zhao X,Schwartz CL,Pierson J,Giovannoni SJ,McIntosh JR,Nicastro D

更新日期：2017-01-17 00:00:00

abstract：:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the ...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG

更新日期：1996-02-01 00:00:00

abstract：:The growing mycelia of Trichoderma reesei Rut-C30 are richly endowed with endoplasmic reticula and a variety of pleomorphic subcellular bodies. Mycelia of the culture growing in presence of avicel pH101 was fractionated in sucrose density gradients, and several morphologically and biochemically distinct fractions were...

journal_title：Applied and environmental microbiology

authors： Glenn M,Ghosh A,Ghosh BK

更新日期：1985-11-01 00:00:00

abstract：:Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA,Grifo AP Jr

更新日期：1989-11-01 00:00:00

abstract：:The natural abundance of stable carbon isotopes measured in bacterial nucleic acids extracted from estuarine bacterial concentrates was used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of Pseudomonas aeruginosa and nucleic acids extracted from cultures rese...

journal_title：Applied and environmental microbiology

authors： Coffin RB,Velinsky DJ,Devereux R,Price WA,Cifuentes LA

更新日期：1990-07-01 00:00:00

abstract：:Heat inactivation of mycobacteria from lesions and from culture was compared in meat products. In vivo-grown organisms were more easily heat inactivated than were in vitro-grown organisms. ...

journal_title：Applied and environmental microbiology

authors： Merkal RS,Lyle PS,Whipple DL

更新日期：1981-06-01 00:00:00

abstract：:Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca or Mg, allowing the release of intact vacuoles in high yield...

journal_title：Applied and environmental microbiology

authors： Keller U,Madry N,Kleinkauf H,Glund K

更新日期：1984-04-01 00:00:00

abstract：:Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

journal_title：Applied and environmental microbiology

authors： Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

更新日期：2002-09-01 00:00:00

abstract：:Ammonia oxidation is a major process in nitrogen cycling, and it plays a key role in nitrogen limited soil ecosystems such as those in the arctic. Although mm-scale spatial dependency of ammonia oxidizers has been investigated, little is known about the field-scale spatial dependency of aerobic ammonia oxidation proce...

journal_title：Applied and environmental microbiology

authors： Banerjee S,Siciliano SD

更新日期：2012-01-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

journal_title：Applied and environmental microbiology

authors： Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

更新日期：2003-06-01 00:00:00