Multiplex reverse transcription-PCR for simultaneous detection of beet necrotic yellow vein virus, Beet soilborne virus, and Beet virus Q and their vector Polymyxa betae KESKIN on sugar beet.

abstract：:A total of 113 strains of mesophilic strains lactic streptococci of the species Streptococcus lactis, S. lactis subsp. diacetilactis, and S. cremoris, chosen from 291 strains that had been previously classified into six groups on the basis of their sensitivity to 132 virulent phages, were subjected to induction with m...

journal_title：Applied and environmental microbiology

authors： Reyrolle J,Chopin MC,Letellier F,Novel G

更新日期：1982-02-01 00:00:00

abstract：:Significant amounts of ethylene were produced by Pseudomonas syringae pv. glycinea, pv. phaseolicola (which had been isolated from viny weed Pueraria lobata [Willd.] Ohwi [common name, kudzu]), and pv. pisi in synthetic medium. On the other hand, the bean strains of P. syringae pv. phaseolicola and strains of 17 other...

journal_title：Applied and environmental microbiology

authors： Weingart H,Volksch B

更新日期：1997-01-01 00:00:00

abstract：:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance. Therefore, benzene metabolism was investigated in Geobacter metallireducens, the only genetically tractable organism known to anaerobically degrade benzene. Trace amounts (<0.5 μM) of phenol accumulated in cult...

journal_title：Applied and environmental microbiology

authors： Zhang T,Tremblay PL,Chaurasia AK,Smith JA,Bain TS,Lovley DR

更新日期：2013-12-01 00:00:00

abstract：:Campylobacter infections are increasing and pose a serious public health problem in Denmark. Infections in humans and broiler flocks show similar seasonality, suggesting that climate may play a role in infection. We examined the effects of temperature, precipitation, relative humidity, and hours of sunlight on Campylo...

journal_title：Applied and environmental microbiology

authors： Patrick ME,Christiansen LE,Wainø M,Ethelberg S,Madsen H,Wegener HC

更新日期：2004-12-01 00:00:00

abstract：:Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Caputo RA,Graham GS

更新日期：1981-06-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. An inulinase-hyperproducing mutant, Aspergillus niger 817, was grown in a submerged culture at 30 degrees C for 5 days. The inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inu...

journal_title：Applied and environmental microbiology

authors： Ohta K,Hamada S,Nakamura T

更新日期：1993-03-01 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:A compact housing unit for low-power (X6 to X50) cinemicrographic studies of microbial specimens in the vertical plane is described. This unit was used successfully to record the development of a "halo" of cells around subsurface colonies of Escherichia coli. ...

journal_title：Applied and environmental microbiology

authors： Wilkins JR,Tynan CI Jr,Boykin EH

更新日期：1976-08-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleoti...

journal_title：Applied and environmental microbiology

authors： Andrew M,Kohn LM

更新日期：2009-09-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:Prior growth of seven enterohemorrhagic and one nonenterohemorrhagic strains of Escherichia coli in tryptic soy broth with (TSB+G) and without (TSB-G) 1% glucose was evaluated for its effect on acid tolerance. The final pHs of 18-h TSB+G and TSB-G cultures were 4.6 to 5.2 and 6.9 to 7.0, respectively. Cells were then ...

journal_title：Applied and environmental microbiology

authors： Buchanan RL,Edelson SG

更新日期：1996-11-01 00:00:00

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were...

journal_title：Applied and environmental microbiology

authors： Johnson AD,Eddy GA,Gangemi JD,Ramsburg HH,Metzger JF

更新日期：1978-02-01 00:00:00

abstract：:Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are highly motile Gram-negative predatory bacteria with the potential of being used as biocontrol agents or living antibiotics. It was suggested previously that sugar-binding proteins play a role in M. aeruginosavorus and B. bacteriovorus host specificity and p...

journal_title：Applied and environmental microbiology

authors： Dashiff A,Keeling TG,Kadouri DE

更新日期：2011-04-01 00:00:00

abstract：:The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

journal_title：Applied and environmental microbiology

authors： Kelso BHL,Smith RV,Laughlin RJ

更新日期：1999-01-01 00:00:00

abstract：:Washed cell suspensions of the facultative methylotroph strain IMB-1 grown on methyl bromide (MeBr) were able to consume methyl chloride (MeCl) and methyl iodide (MeI) as well as MeBr. Consumption of >100 microM MeBr by cells grown on glucose, acetate, or monomethylamine required induction. Induction was inhibited by ...

journal_title：Applied and environmental microbiology

authors： Schaefer JK,Oremland RS

更新日期：1999-11-01 00:00:00

abstract：:Recent isolation of Pseudomonas aeruginosa strains from the open ocean and subsequent pulsed-field gel electrophoresis analyses indicate that these strains have a unique genotype (N. H. Khan, Y. Ishii, N. Kimata-Kino, H. Esaki, T. Nishino, M. Nishimura, and K. Kogure, Microb. Ecol. 53:173-186, 2007). We hypothesized t...

journal_title：Applied and environmental microbiology

authors： Khan NH,Ahsan M,Yoshizawa S,Hosoya S,Yokota A,Kogure K

更新日期：2008-10-01 00:00:00

abstract：:Phage therapy is being reexamined as a strategy for bacterial control in medical and other environments. As microorganisms often live in mixed populations, we examined the effect of Escherichia coli bacteriophage λW60 and Pseudomonas aeruginosa bacteriophage PB-1 infection on the viability of monoculture and mixed-spe...

journal_title：Applied and environmental microbiology

authors： Kay MK,Erwin TC,McLean RJ,Aron GM

更新日期：2011-02-01 00:00:00

abstract：:The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

journal_title：Applied and environmental microbiology

authors： Sasaki T,Ohshima Y

更新日期：1987-07-01 00:00:00

abstract：:Twenty fungal genera, including 14 Fusarium species, were examined for ice nucleation activity at -5.0 degrees C, and this activity was found only in Fusarium acuminatum and Fusarium avenaceum. This characteristic is unique to these two species. Ice nucleation activity of F. avenaceum was compared with ice nucleation ...

journal_title：Applied and environmental microbiology

authors： Pouleur S,Richard C,Martin JG,Antoun H

更新日期：1992-09-01 00:00:00

abstract：:A bacterial biosensor for benzene, toluene, and similar compounds has been constructed, characterized, and field tested on contaminated water and soil. The biosensor is based on a plasmid incorporating the transcriptional activator xylR from the TOL plasmid of Pseudomonas putida mt-2. The XylR protein binds a subset o...

journal_title：Applied and environmental microbiology

authors： Willardson BM,Wilkins JF,Rand TA,Schupp JM,Hill KK,Keim P,Jackson PJ

更新日期：1998-03-01 00:00:00

abstract：:The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

journal_title：Applied and environmental microbiology

authors： McMeekin TA

更新日期：1977-06-01 00:00:00

abstract：:Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

journal_title：Applied and environmental microbiology

authors： Stupperich E,Konle R

更新日期：1993-09-01 00:00:00

abstract：:Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen Vibrio anguillarum. The aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a c...

journal_title：Applied and environmental microbiology

authors： Olsson JC,Westerdahl A,Conway PL,Kjelleberg S

更新日期：1992-02-01 00:00:00

abstract：:Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...

journal_title：Applied and environmental microbiology

authors： Kolkman MA,van der Ploeg R,Bertels M,van Dijk M,van der Laan J,van Dijl JM,Ferrari E

更新日期：2008-12-01 00:00:00

abstract：:Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

journal_title：Applied and environmental microbiology

authors： Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

更新日期：2005-12-01 00:00:00

abstract：:We have cloned and sequenced the Cercospora nicotianae gene for the carotenoid biosynthetic enzyme phytoene dehydrogenase. Analysis of the derived amino acid sequence revealed it has greater than 50% identity with its counterpart in Neurospora crassa and approximately 30% identity with prokaryotic phytoene dehydrogena...

journal_title：Applied and environmental microbiology

authors： Ehrenshaft M,Daub ME

更新日期：1994-08-01 00:00:00