Specimen housing unit for cinemicrographic studies in the vertical plane.

abstract：:The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

journal_title：Applied and environmental microbiology

authors： Abe J,Ushijima C,Hizukuri S

更新日期：1999-09-01 00:00:00

abstract：:Both the wild type and an isogenic hydrogenase-negative mutant of Azorhizobium caulinodans growing ex planta on N(2) as the N source were studied in succinate-limited steady-state chemostat cultures under 0.2 to 3.0% dissolved O(2) tension. Production or consumption of O(2), H(2), and CO(2) was measured with an on-lin...

journal_title：Applied and environmental microbiology

authors： Boogerd FC,Ferdinandy-van Vlerken MM,Mawadza C,Pronk AF,Stouthamer AH,van Verseveld HW

更新日期：1994-06-01 00:00:00

abstract：:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch an...

journal_title：Applied and environmental microbiology

authors： Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H

更新日期：2003-06-01 00:00:00

abstract：:Leuconostoc mesenteroides strains that are resistant to high levels of nisin (up to 25,000 IU/ml in broth) were isolated. These nisin-resistant mutants were evaluated to determine their potential use as starter culture strains for cabbage fermentations. We found that some L. mesenteroides strains could be adapted to h...

journal_title：Applied and environmental microbiology

authors： Breidt F,Crowley KA,Fleming HP

更新日期：1993-11-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

journal_title：Applied and environmental microbiology

authors： Jürgens K,Pernthaler J,Schalla S,Amann R

更新日期：1999-03-01 00:00:00

abstract：:In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly c...

journal_title：Applied and environmental microbiology

authors： Picard C,Ponsonnet C,Paget E,Nesme X,Simonet P

更新日期：1992-09-01 00:00:00

abstract：:Vibrio fischeri colonizes the squid Euprymna scolopes in a mutualistic symbiosis. Hatchling squid lack these bacterial symbionts, and V. fischeri strains must compete to occupy this privileged niche. We cloned a V. fischeri gene, designated pilA, that contributes to colonization competitiveness and encodes a protein s...

journal_title：Applied and environmental microbiology

authors： Stabb EV,Ruby EG

更新日期：2003-02-01 00:00:00

abstract：:In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

journal_title：Applied and environmental microbiology

authors： Abrahamsen LH,Jerkofsky M

更新日期：1981-03-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00

abstract：:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

journal_title：Applied and environmental microbiology

authors： Clough SJ,Flavier AB,Schell MA,Denny TP

更新日期：1997-03-01 00:00:00

abstract：:This study measured the environmental variability which exists in a commercial aerobic composting process. The specific process studied is carried out in six decomposition cells which present six different phases of the process. Temperature, pH, and moisture content were determined in several randomly chosen sample si...

journal_title：Applied and environmental microbiology

authors： Atchley SH,Clark JB

更新日期：1979-12-01 00:00:00

abstract：:Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

journal_title：Applied and environmental microbiology

authors： Stupperich E,Konle R

更新日期：1993-09-01 00:00:00

abstract：:By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tryptophan residues i...

journal_title：Applied and environmental microbiology

authors： Vaiphei ST,Mao L,Shimazu T,Park JH,Inouye M

更新日期：2010-09-01 00:00:00

abstract：:Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...

journal_title：Applied and environmental microbiology

authors： Oladeinde A,Bohrmann T,Wong K,Purucker ST,Bradshaw K,Brown R,Snyder B,Molina M

更新日期：2014-01-01 00:00:00

abstract：:A mechanistic understanding of how new phenotypes develop to overcome the loss of a gene product provides valuable insight on both the metabolic and regulatory functions of the lost gene. The pgi gene, whose product catalyzes the second step in glycolysis, was deleted in a growth-optimized Escherichia coli K-12 MG1655...

journal_title：Applied and environmental microbiology

authors： McCloskey D,Xu S,Sandberg TE,Brunk E,Hefner Y,Szubin R,Feist AM,Palsson BO

更新日期：2018-09-17 00:00:00

abstract：:We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lacto...

journal_title：Applied and environmental microbiology

authors： Waché Y,Laroche C,Bergmark K,Møller-Andersen C,Aguedo M,Le Dall MT,Wang H,Nicaud JM,Belin JM

更新日期：2000-03-01 00:00:00

abstract：:Corynebacterium glutamicum is able to grow in media containing up to 12 mM arsenite and 500 mM arsenate and is one of the most arsenic-resistant microorganisms described to date. Two operons (ars1 and ars2) involved in arsenate and arsenite resistance have been identified in the complete genome sequence of Corynebacte...

journal_title：Applied and environmental microbiology

authors： Ordóñez E,Letek M,Valbuena N,Gil JA,Mateos LM

更新日期：2005-10-01 00:00:00

abstract：:Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

journal_title：Applied and environmental microbiology

authors： Schmidt T,Stoppel RD,Schlegel HG

更新日期：1991-11-01 00:00:00

abstract：:Bacteroides fragilis subsp. fragilis was maintained in a chemostat modified for anaerobic conditions to test the effects of dissolved oxygen and Eh on growth. Using a defined medium containing glucose and a dilution rate of 0.16 h -1, a stable population of 3 X 10(9) colony-forming units/ml was present. At this steady...

journal_title：Applied and environmental microbiology

authors： Onderdonk AB,Johnston J,Mayhew JW,Gorbach SL

更新日期：1976-02-01 00:00:00

abstract：:Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis s...

journal_title：Applied and environmental microbiology

authors： Aronson JN,Arvidson HC

更新日期：1987-02-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Laboratory conditions have been identified that cause the rapid death of cultures of cyanobacteria producing urease. Once the death phase had initiated in the stationary growth phase, cells were rapidly bleached of all pigmentation. Null mutations in the ureC gene, encoding the alpha subunit of urease, were constructe...

journal_title：Applied and environmental microbiology

authors： Sakamoto T,Delgaizo VB,Bryant DA

更新日期：1998-07-01 00:00:00

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:The influence of nisin on the proton motive force (delta p) generated by glucose-energized cells of the obligate putrefactive anaerobe Clostridium sporogenes PA 3679 was determined. The components of delta p, the transmembrane potential (delta psi) and the pH gradient (delta pH), were determined from the distributions...

journal_title：Applied and environmental microbiology

authors： Okereke A,Montville TJ

更新日期：1992-08-01 00:00:00

abstract：:An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This ra...

journal_title：Applied and environmental microbiology

authors： Mah RA,Smith MR,Baresi L

更新日期：1978-06-01 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1995-12-01 00:00:00

abstract：:Bacterioplankton numbers, biomasses, and productivities, as well as chlorophyll a concentrations and phytoplankton productivities, were assayed from 1 March 1984 to 12 August 1985 through a 250-m-deep seawater column in Howe Sound, a temperate fjord-sound on the southern coast of British Columbia, Canada. Primary prod...

journal_title：Applied and environmental microbiology

authors： Albright LJ,McCrae SK

更新日期：1987-06-01 00:00:00