Nisin dissipates the proton motive force of the obligate anaerobe Clostridium sporogenes PA 3679.

abstract：:Campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by Campylobacter coli. Most studies have concentrated on Campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examin...

journal_title：Applied and environmental microbiology

authors： Leatherbarrow AJ,Hart CA,Kemp R,Williams NJ,Ridley A,Sharma M,Diggle PJ,Wright EJ,Sutherst J,French NP

更新日期：2004-02-01 00:00:00

abstract：:The genus Listeria comprises food-borne pathogens associated with severe infections and a high mortality rate. Endolysins from bacteriophages infecting Listeria are promising tools for both their detection and control. These proteins feature a modular organization, consisting of an N-terminal enzymatically active doma...

journal_title：Applied and environmental microbiology

authors： Schmelcher M,Shabarova T,Eugster MR,Eichenseher F,Tchang VS,Banz M,Loessner MJ

更新日期：2010-09-01 00:00:00

abstract：:An efficient insertion mutagenesis strategy for bacterial genomes based on the phage Mu DNA transposition reaction was developed. Incubation of MuA transposase protein with artificial mini-Mu transposon DNA in the absence of divalent cations in vitro resulted in stable but inactive Mu DNA transposition complexes, or t...

journal_title：Applied and environmental microbiology

authors： Lamberg A,Nieminen S,Qiao M,Savilahti H

更新日期：2002-02-01 00:00:00

abstract：:Although it is widely believed that horizontal patchiness exists in microbial sediment communities, determining the extent of variability or the particular members of the bacterial community which account for the observed differences among sites at various scales has not been routinely demonstrated. In this study, hor...

journal_title：Applied and environmental microbiology

authors： Scala DJ,Kerkhof LJ

更新日期：2000-05-01 00:00:00

abstract：:Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the...

journal_title：Applied and environmental microbiology

authors： Schel AJ,Marsh PD,Bradshaw DJ,Finney M,Fulford MR,Frandsen E,Østergaard E,ten Cate JM,Moorer WR,Mavridou A,Kamma JJ,Mandilara G,Stösser L,Kneist S,Araujo R,Contreras N,Goroncy-Bermes P,O'Mullane D,Burke F,O'Reilly P

更新日期：2006-02-01 00:00:00

abstract：:Strains of Bifidobacterium animalis subsp. lactis are extensively exploited by the food industry as health-promoting bacteria, although the genetic variability of members belonging to this taxon has so far not received much scientific attention. In this article, we describe the complete genetic makeup of the B. animal...

journal_title：Applied and environmental microbiology

authors： Milani C,Duranti S,Lugli GA,Bottacini F,Strati F,Arioli S,Foroni E,Turroni F,van Sinderen D,Ventura M

更新日期：2013-07-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus can grow with short- to long-chain fatty acids as the sole carbon source (R. G. Kranz, K. K. Gabbert, T. A. Locke, and M. T. Madigan, Appl. Environ. Microbiol. 63:3003-3009, 1997). Concomitant with growth on fatty acids is the production to high levels of the polyest...

journal_title：Applied and environmental microbiology

authors： Kranz RG,Gabbert KK,Madigan MT

更新日期：1997-08-01 00:00:00

abstract：:Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (...

journal_title：Applied and environmental microbiology

authors： Délye C,Ronchi V,Laigret F,Corio-Costet MF

更新日期：1999-09-01 00:00:00

abstract：:In prokaryotic cells the ATP-dependent proteases Lon and ClpP (Clp proteolytic subunit) are involved in the turnover of misfolded proteins and the degradation of regulatory proteins, and depending on the organism, these proteases contribute variably to stress tolerance. We constructed mutants in the lon and clpP genes...

journal_title：Applied and environmental microbiology

authors： Cohn MT,Ingmer H,Mulholland F,Jørgensen K,Wells JM,Brøndsted L

更新日期：2007-12-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:Streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. Serotyping is an important tool for detection and epidemiological studies of S. suis Thirty-three reference serotypes and nine novel cps loci (NCLs) are recognized in S. suis To gain a better understanding of the prevalence an...

journal_title：Applied and environmental microbiology

authors： Qiu X,Bai X,Lan R,Zheng H,Xu J

更新日期：2016-11-21 00:00:00

abstract：:Over 1,400 water samples were collected biweekly over 6 years from an intermittent stream protected and unprotected from pasturing cattle. The samples were monitored for host-specific Bacteroidales markers, Cryptosporidium species/genotypes, viruses and coliphages associated with humans or animals, and bacterial zoono...

journal_title：Applied and environmental microbiology

authors： Wilkes G,Brassard J,Edge TA,Gannon V,Jokinen CC,Jones TH,Marti R,Neumann NF,Ruecker NJ,Sunohara M,Topp E,Lapen DR

更新日期：2013-10-01 00:00:00

abstract：:Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. s...

journal_title：Applied and environmental microbiology

authors： Asaka O,Shoda M

更新日期：1996-11-01 00:00:00

abstract：UNLABELLED:We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrat...

journal_title：Applied and environmental microbiology

authors： Wilding M,Peat TS,Newman J,Scott C

更新日期：2016-06-13 00:00:00

abstract：:An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...

journal_title：Applied and environmental microbiology

authors： Schneider J,Fricke C,Overwin H,Hofmann B,Hofer B

更新日期：2009-12-01 00:00:00

abstract：:Prophages make up 12% of the enterohemorrhagic Escherichia coli genome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among ...

journal_title：Applied and environmental microbiology

authors： Chen C,Lewis CR,Goswami K,Roberts EL,DebRoy C,Dudley EG

更新日期：2013-03-01 00:00:00

abstract：:The recalcitrance of xenobiotics may be caused by an absence of transforming enzymes or by their inability to enter microbial cells. A nondestructive method for differentiating between these two possibilities is described. The solid n-alkanes octadecane (C(18)) and hexatriacontane (C(36)) were encapsulated into phosph...

journal_title：Applied and environmental microbiology

authors： Miller RM,Bartha R

更新日期：1989-02-01 00:00:00

abstract：:Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

journal_title：Applied and environmental microbiology

authors： Diemert S,Yan T

更新日期：2019-05-02 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:Systematic environmental surveillance for poliovirus circulation has been conducted in Egypt since 2000. The surveillance has revealed three independent importations of wild-type poliovirus. In addition, several vaccine-derived polioviruses have been detected in various locations in Egypt. In addition to acute flaccid...

journal_title：Applied and environmental microbiology

authors： Blomqvist S,El Bassioni L,El Maamoon Nasr EM,Paananen A,Kaijalainen S,Asghar H,de Gourville E,Roivainen M

更新日期：2012-08-01 00:00:00

abstract：:Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

journal_title：Applied and environmental microbiology

authors： Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

更新日期：2012-12-01 00:00:00

abstract：:Diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. In this study, a long-term placebo-controlled cro...

journal_title：Applied and environmental microbiology

authors： Vanhoutte T,De Preter V,De Brandt E,Verbeke K,Swings J,Huys G

更新日期：2006-09-01 00:00:00

abstract：:Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil...

journal_title：Applied and environmental microbiology

authors： Pontiroli A,Rizzi A,Simonet P,Daffonchio D,Vogel TM,Monier JM

更新日期：2009-05-01 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00

abstract：UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

journal_title：Applied and environmental microbiology

authors： Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

更新日期：2016-07-29 00:00:00

abstract：:A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glyci...

journal_title：Applied and environmental microbiology

authors： Juni E,Heym GA,Avery M

更新日期：1986-09-01 00:00:00

abstract：:Flavonoids are 15-carbon plant secondary metabolites exuded in the rhizosphere that hosts several flavonoid-degrading bacteria. We studied flavonoid catabolism in a plant growth-promoting rhizobacterial strain of Pseudomonas by using a combination of biochemical and genetic approaches. Transposants carrying mini-Tn5gf...

journal_title：Applied and environmental microbiology

authors： Pillai BV,Swarup S

更新日期：2002-01-01 00:00:00

abstract：:The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

journal_title：Applied and environmental microbiology

authors： Wilkinson TL,Koga R,Fukatsu T

更新日期：2007-02-01 00:00:00