Coherence among different microbial source tracking markers in a small agricultural stream with or without livestock exclusion practices.

abstract：:The gene encoding the beta-amylase of Bacillus cereus BQ10-S1 (SpoII) was cloned into Escherichia coli JM 109. A sequenced DNA fragment of 2,001 bp contains the beta-amylase gene. The N-terminal sequences (AVNGKG MNPDYKAYLMAPLKKI), the C-terminal sequences (SHTSSW), and the amino acid sequences of the five regions in ...

journal_title：Applied and environmental microbiology

authors： Nanmori T,Nagai M,Shimizu Y,Shinke R,Mikami B

更新日期：1993-02-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...

journal_title：Applied and environmental microbiology

authors： Limsawat S,Ohgaki S

更新日期：1997-07-01 00:00:00

abstract：:Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

journal_title：Applied and environmental microbiology

authors： Klinke S,Dauner M,Scott G,Kessler B,Witholt B

更新日期：2000-03-01 00:00:00

abstract：:Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

journal_title：Applied and environmental microbiology

authors： Millard CS,Chao YP,Liao JC,Donnelly MI

更新日期：1996-05-01 00:00:00

abstract：:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless bet...

journal_title：Applied and environmental microbiology

authors： Binishofer B,Moll I,Henrich B,Bläsi U

更新日期：2002-08-01 00:00:00

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

journal_title：Applied and environmental microbiology

authors： Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

更新日期：2018-08-17 00:00:00

abstract：:Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...

journal_title：Applied and environmental microbiology

authors： Knudsen GM,Ng Y,Gram L

更新日期：2013-12-01 00:00:00

abstract：:Copper resistance mechanisms provide an important adaptive advantage to plant pathogenic bacteria under exposure to copper treatments. Copper resistance determinants have been described in Pseudomonas syringae pv. syringae (Pss) strains isolated from mango intimately associated with 62 kb plasmids belonging to the pPT...

journal_title：Applied and environmental microbiology

authors： Aprile F,Heredia-Ponce Z,Cazorla FM,de Vicente A,Gutiérrez-Barranquero JA

更新日期：2020-12-23 00:00:00

abstract：:In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S ...

journal_title：Applied and environmental microbiology

authors： Duranti S,Turroni F,Milani C,Foroni E,Bottacini F,Dal Bello F,Ferrarini A,Delledonne M,van Sinderen D,Ventura M

更新日期：2013-01-01 00:00:00

abstract：:The role of light as an energy source for slightly aereated cultures of halobacteria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non-illuminated and differently illuminated cultures and with different oxygen transfer rat...

journal_title：Applied and environmental microbiology

authors： Rodriguez-Valera F,Nieto JJ,Ruiz-Berraquero F

更新日期：1983-03-01 00:00:00

abstract：:The Association of Official Analytical Chemists (AOAC) test for assessing the tuberculocidal activity of disinfectants has been shown to be variable. A modified AOAC test, which substituted Middlebrook 7H9 broth as the primary subculture medium and used neutralization by dilution, was compared with the standard AOAC m...

journal_title：Applied and environmental microbiology

authors： Cole EC,Rutala WA,Nessen L,Wannamaker NS,Weber DJ

更新日期：1990-06-01 00:00:00

abstract：:Crenarchaeol, a membrane-spanning glycerol dialkyl glycerol tetraether (GDGT) containing a cyclohexane moiety in addition to four cyclopentane moieties, was originally hypothesized to be synthesized exclusively by the mesophilic Crenarchaeota. Recent studies reporting the occurrence of crenarchaeol in hot springs and ...

journal_title：Applied and environmental microbiology

authors： Pitcher A,Schouten S,Sinninghe Damsté JS

更新日期：2009-07-01 00:00:00

abstract：:Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with...

journal_title：Applied and environmental microbiology

authors： Orrison LH,Cherry WB,Fliermans CB,Dees SB,McDougal LK,Dodd DJ

更新日期：1981-07-01 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...

journal_title：Applied and environmental microbiology

authors： Wang X,Yu P,Zeng C,Ding H,Li Y,Wang C,Lu A

更新日期：2015-08-15 00:00:00

abstract：:Crude extracts from 14 species of sulfate-reducing bacteria comprising the genera Desulfovibrio, Desulfotomaculum, Desulfobulbus, and Desulfosarcina and from three species of sulfide-oxidizing bacteria were tested in an enzyme-linked immunosorbent assay with polyclonal antisera to adenosine 5'-phosphosulfate reductase...

journal_title：Applied and environmental microbiology

authors： Odom JM,Jessie K,Knodel E,Emptage M

更新日期：1991-03-01 00:00:00

abstract：:This study reports on an emerging fungal disease of the edible crab, Cancer pagurus. Juvenile (prerecruit) crabs were found to be subject to this disease condition during the months of May to September at two intertidal sites in South Wales, United Kingdom. Histopathology revealed that the fungi overwhelm the host res...

journal_title：Applied and environmental microbiology

authors： Smith AL,Hamilton KM,Hirschle L,Wootton EC,Vogan CL,Pope EC,Eastwood DC,Rowley AF

更新日期：2013-02-01 00:00:00

abstract：:The 3-phenoxybenzoate (3-PBA) 1',2'-dioxygenase gene cluster (pbaA1A2B cluster), which is responsible for catalyzing 3-phenoxybenzoate to 3-hydroxybenzoate and catechol, is inducibly expressed in Sphingobium wenxiniae strain JZ-1(T) by its substrate 3-PBA. In this study, we identified a transcriptional activator of th...

journal_title：Applied and environmental microbiology

authors： Cheng M,Chen K,Guo S,Huang X,He J,Li S,Jiang J

更新日期：2015-12-01 00:00:00

abstract：:Factors affecting the production of [14C]patulin from [1-14C]acetate by replacement cultures of Penicillium patulum have been investigated. Incorporation of [1-14C]acetate into patulin reached a maximum with 6- to 8-day-old cultures incubated at 28 degrees C for 8 h in a replacement medium containing 0.1 M glucose, in...

journal_title：Applied and environmental microbiology

authors： Nip WK,Chu FS

更新日期：1977-04-01 00:00:00

abstract：:Simkania negevensis is a recently discovered chlamydia-like intracellular microorganism which has been associated with bronchiolitis in infants and with community-acquired pneumonia in adults; a high seroprevalence of antibodies to the microorganism has been found in various population groups. S. negevensis can be gro...

journal_title：Applied and environmental microbiology

authors： Kahane S,Platzner N,Dvoskin B,Itzhaki A,Friedman MG

更新日期：2004-06-01 00:00:00

abstract：:Rhizobium fredii USDA 206 harbors four large plasmids, one of which carries nodulation and nitrogen fixation genes. Previously isolated groups of plasmid-cured derivatives of strain USDA 206 were compared with each other to determine possible plasmid functions. Mutant strain 206CANS was isolated as a nonmucoid (Muc) d...

journal_title：Applied and environmental microbiology

authors： Barbour WM,Elkan GH

更新日期：1989-04-01 00:00:00

abstract：:Viruses are now recognized as a key component in pelagic systems, but their role in marine sediment has yet to be assessed. In this study bacterial and viral densities were determined at nine deep-sea stations selected from three main sites (i.e., the Sporades Basin, the Cretan Sea, and the Ierapetra Trench at depths ...

journal_title：Applied and environmental microbiology

authors： Danovaro R,Serresi M

更新日期：2000-05-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:A total of 85 isolates of mesophilic, aerobic, heterotrophic bacteria were isolated from the gut, peristomial membrane, and coelomic fluid from specimens of the sea urchin Echinus esculentus from the Clyde Sea area of Scotland. These isolates were compared with 26 isolates from sand and seawater in the same locality. ...

journal_title：Applied and environmental microbiology

authors： Unkles SE

更新日期：1977-10-01 00:00:00

abstract：:Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...

journal_title：Applied and environmental microbiology

authors： Amner W,McCarthy AJ,Edwards C

更新日期：1988-12-01 00:00:00

abstract：:A toxic substance contained in the blue-green alga Microcystis aeruginosa was purified and partially characterized. Toxic algal cells were collected from a highly eutrophic lake in Japan, and the toxin was purified by homogenization, ultrafiltration, gel filtration, and ion-exchange chromatography. The final preparati...

journal_title：Applied and environmental microbiology

authors： Watanabe MF,Oishi S

更新日期：1982-04-01 00:00:00

abstract：:Inhibition of Aspergillus flavus growth from spores was used as a simple bioassay for toxicity of Al and Mn mobilized by simulated acid precipitation. Al was identified as being toxic in soil leachates resulting from acid inputs of pH less than 2.7. Inhibition by Mn was not detectable. The addition of fluoride signifi...

journal_title：Applied and environmental microbiology

authors： Firestone MK,Killham K,McColl JG

更新日期：1983-09-01 00:00:00