Abstract:
:A toxic substance contained in the blue-green alga Microcystis aeruginosa was purified and partially characterized. Toxic algal cells were collected from a highly eutrophic lake in Japan, and the toxin was purified by homogenization, ultrafiltration, gel filtration, and ion-exchange chromatography. The final preparation gave a single peak on high-performance liquid chromatography. The toxicity was somewhat less than that reported for other toxins from this alga. The water extract of 6.7 mg (dry weight) of cells and 72 microgram of the purified protein was required to kill a mouse (1 mouse unit). The main amino acids of the toxin were glutamic acid, asparatic acid, alanine, glycine, arginine, and leucine. The molecular weight of the toxin was 2,950 as determined by high-performance liquid chromatography.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Watanabe MF,Oishi Sdoi
10.1128/AEM.43.4.819-822.1982subject
Has Abstractpub_date
1982-04-01 00:00:00pages
819-22issue
4eissn
0099-2240issn
1098-5336journal_volume
43pub_type
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