Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.


:Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. Percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis A virus, 77 for human rotavirus Wa, 87 for simian rotavirus SA11, and 68 for poliovirus. Percent recoveries of 97, 40, 97 and 105, respectively, for the same viruses were obtained from oyster eluates by the same procedure. Percent recoveries of 97 for hepatitis A virus and 78 for human rotavirus Wa were obtained from sediment eluates containing 2 M NaNO3 with a final concentration of 15% polyethylene glycol 6000. The polyethylene glycol method was shown to be more effective than the organic flocculation method for recovery of hepatitis A virus and rotaviruses Wa and SA11, but not of poliovirus 1 in laboratory studies. In field trials, hepatitis A virus or rotavirus or both were recovered from 12 of 18 eluates by polyethylene glycol, compared with recovery from 9 of 18 eluates by organic flocculation from fresh and estuarine waters subject to pollution.


Appl Environ Microbiol


Lewis GD,Metcalf TG




Has Abstract


1988-08-01 00:00:00












  • Improved transferase/hydrolase ratio through rational design of a family 1 β-glucosidase from Thermotoga neapolitana.

    abstract::Alkyl glycosides are attractive surfactants because of their high surface activity and good biodegradability and can be produced from renewable resources. Through enzymatic catalysis, one can obtain well-defined alkyl glycosides, something that is very difficult to do using conventional chemistry. However, there is a ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lundemo P,Adlercreutz P,Karlsson EN

    更新日期:2013-06-01 00:00:00

  • Identification and characterization of a novel CprA reductive dehalogenase specific to highly chlorinated phenols from Desulfitobacterium hafniense strain PCP-1.

    abstract::Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bisaillon A,Beaudet R,Lépine F,Déziel E,Villemur R

    更新日期:2010-11-01 00:00:00

  • Abundance and diversity of soybean-nodulating rhizobia in black soil are impacted by land use and crop management.

    abstract::To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

    更新日期:2014-09-01 00:00:00

  • Role of an essential acyl coenzyme A carboxylase in the primary and secondary metabolism of Streptomyces coelicolor A3(2).

    abstract::Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

    更新日期:2001-09-01 00:00:00

  • Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-19.

    abstract::Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

    更新日期:1999-11-01 00:00:00

  • Determination of the concentration of maltose- and starch-like compounds in drinking water by growth measurements with a well-defined strain of a Flavobacterium species.

    abstract::The growth kinetics of Flavobacterium sp. strain S12 specialized in the utilization of glycerol, and a number of oligo- and polysaccharides were determined in batch-culture experiments at 15 degrees C in pasteurized tap water supplied with very low amounts of substrates. Kss for the growth on maltotriose, maltotetraos...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: van der Kooij D,Hijnen WA

    更新日期:1985-04-01 00:00:00

  • Superoxide dismutase activity during recovery of thermally stressed Staphylococcus aureus MF-31.

    abstract::Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bucker ER,Martin SE

    更新日期:1981-03-01 00:00:00

  • Characterization of a latent virus-like infection of symbiotic zooxanthellae.

    abstract::A latent virus-like agent, which we designated zooxanthella filamentous virus 1 (ZFV1), was isolated from Symbiodinium sp. strain CCMP 2465 and characterized. Transmission electron microscopy and analytical flow cytometry revealed the presence of a new group of distinctive filamentous virus-like particles after exposu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lohr J,Munn CB,Wilson WH

    更新日期:2007-05-01 00:00:00

  • Glutathione protects Lactococcus lactis against oxidative stress.

    abstract::Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to approximately 60 mM glutathione when this compound was added to the medium. S...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Li Y,Hugenholtz J,Abee T,Molenaar D

    更新日期:2003-10-01 00:00:00

  • Bartonella chomelii is the most frequent species infecting cattle grazing in communal mountain pastures in Spain.

    abstract::The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n = 133) or horses (n = 91) were found not to be infecte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Antequera-Gómez ML,Lozano-Almendral L,Barandika JF,González-Martín-Niño RM,Rodríguez-Moreno I,García-Pérez AL,Gil H

    更新日期:2015-01-01 00:00:00

  • DNA amplification polymorphisms of the cultivated mushroom Agaricus bisporus.

    abstract::Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Khush RS,Becker E,Wach M

    更新日期:1992-09-01 00:00:00

  • Ethanol Inhibition Kinetics of Kluyveromyces marxianus Grown on Jerusalem Artichoke Juice.

    abstract::The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bajpai P,Margaritis A

    更新日期:1982-12-01 00:00:00

  • Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

    abstract::A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Delécluse A,Rosso ML,Ragni A

    更新日期:1995-12-01 00:00:00

  • Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp.

    abstract::Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Berk SG,Ting RS,Turner GW,Ashburn RJ

    更新日期:1998-01-01 00:00:00

  • AidH, an alpha/beta-hydrolase fold family member from an Ochrobactrum sp. strain, is a novel N-acylhomoserine lactonase.

    abstract::N-acylhomoserine lactones (AHLs) are signaling molecules in many quorum-sensing (QS) systems that regulate interactions between various pathogenic bacteria and their hosts. Quorum quenching by the enzymatic inactivation of AHLs holds great promise in preventing and treating infections, and several such enzymes have be...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mei GY,Yan XX,Turak A,Luo ZQ,Zhang LQ

    更新日期:2010-08-01 00:00:00

  • Isolation of tellurite- and selenite-resistant bacteria from hydrothermal vents of the Juan de Fuca Ridge in the Pacific Ocean.

    abstract::Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

    更新日期:2002-09-01 00:00:00

  • Evidence of the internalization of animal caliciviruses via the roots of growing strawberry plants and dissemination to the fruit.

    abstract::Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: DiCaprio E,Culbertson D,Li J

    更新日期:2015-04-01 00:00:00

  • Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response.

    abstract::Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

    更新日期:2007-10-01 00:00:00

  • Rapid spectrophotometric differentiation between glutathione-dependent and glutathione-independent gentisate and homogentisate pathways.

    abstract::A total of four pathways are known for the catabolism by microorganisms of gentisate (2,5-dihydroxybenzoate) and homogentisate (2,5-dihydroxyphenylacetate). Both of these dihydric phenols can be degraded by either a glutathione-dependent or a glutathione-independent reaction sequence. We found that it is not always po...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Crawford RL,Frick TD

    更新日期:1977-08-01 00:00:00

  • The gene yggE functions in restoring physiological defects of Escherichia coli cultivated under oxidative stress conditions.

    abstract::DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 ve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya M

    更新日期:2005-05-01 00:00:00

  • Types and distribution of anaerobic bacteria in the large intestine of pigs.

    abstract::An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Russell EG

    更新日期:1979-02-01 00:00:00

  • Liposomes replace serum for cultivation of fermenting mycoplasmas.

    abstract::Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Cluss RG,Johnson JK,Somerson NL

    更新日期:1983-08-01 00:00:00

  • Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments.

    abstract::A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations of these bacteria (designated MAR 1) are persistent and widespread, spanning at least three distinct ocean systems. In this study, 212 actinomycete isolates possessing MAR 1 morphologies wer...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mincer TJ,Jensen PR,Kauffman CA,Fenical W

    更新日期:2002-10-01 00:00:00

  • Genomic investigation of lysogen formation and host lysis systems of the Salmonella temperate bacteriophage SPN9CC.

    abstract::To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Shin H,Lee JH,Yoon H,Kang DH,Ryu S

    更新日期:2014-01-01 00:00:00

  • Ammonia-oxidizing bacteria in a chloraminated distribution system: seasonal occurrence, distribution and disinfection resistance.

    abstract::Nitrification in chloraminated drinking water can have a number of adverse effects on water quality, including a loss of total chlorine and ammonia-N and an increase in the concentration of heterotrophic plate count bacteria and nitrite. To understand how nitrification develops, a study was conducted to examine the fa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wolfe RL,Lieu NI,Izaguirre G,Means EG

    更新日期:1990-02-01 00:00:00

  • Plate diffusion assay as a rapid method for dosimetry of mutagens.

    abstract::This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Awerbuch TE,Stark AA

    更新日期:1979-12-01 00:00:00

  • Construction and behavior of biologically contained bacteria for environmental applications in bioremediation.

    abstract::The survival of microorganisms can be predicted through the use of active biological containment systems. We have constructed contained Pseudomonas putida strains that degrade alkylbenzoates. The modified strain carries a fusion of the Plac promoter to the gef gene, which encodes a killing protein. Expression from Pla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ronchel MC,Ramos C,Jensen LB,Molin S,Ramos JL

    更新日期:1995-08-01 00:00:00

  • Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.

    abstract::The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sandu C,Chiribau CB,Sachelaru P,Brandsch R

    更新日期:2005-12-01 00:00:00

  • Efficient production of polymyxin in the surrogate host Bacillus subtilis by introducing a foreign ectB gene and disrupting the abrB gene.

    abstract::In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Park SY,Choi SK,Kim J,Oh TK,Park SH

    更新日期:2012-06-01 00:00:00

  • Empirical testing of 16S rRNA gene PCR primer pairs reveals variance in target specificity and efficacy not suggested by in silico analysis.

    abstract::Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Morales SE,Holben WE

    更新日期:2009-05-01 00:00:00